Research Article

Related GMR Articles

07/25/2014
Crocodiles; Cross-species amplification; Microsatellites; Neotropical crocodiles

We tested microsatellites that were developed for the saltwater crocodile (Crocodylus porosus) for cross-species amplification and to provide an estimate of inter- and intraspecific variation among four species of Neotropical crocodiles (C. rhombifer, C. intermedius, C. acutus, and C. moreletii). Our results indicated that with the exception of ... more

A. Bashyal; B.A. Gross; M. Venegas-Anaya; F. Lowrance; L.D. Densmore
01/28/2014
Atlantic Forest; Cariniana legalis; Jequitibá rosa; Microsatellites; Spatial genetic structure

The fragments of the Atlantic Forest of southern Bahia have a long history of intense logging and selective cutting. Some tree species, such as jequitibá rosa (Cariniana legalis), have experienced a reduction in their populations with respect to both area and density. To evaluate the possible effects of selective logging on genetic diversity, gene flow, and spatial genetic ... more

J.B. Leal; R.P. Santos; F.A. Gaiotto
05/22/2015
Demographic changes; Genetic heterogeneity; Microsatellites; Mitochondrial sequences; Tayassu pecari

We sequenced the mitochondrial DNA (mtDNA) control region of 59 peccaries (44 white-lipped peccaries, Tayassu pecari, and 15 collared peccaries, Pecari tajacu). We also genotyped 3 DNA microsatellites from 78 white-lipped peccaries representing the 4 putative morphological subspecies (i.e., spiradens, aequatoris, pecari, and albirostris ... more

M. Ruiz-García; M. Pinedo-Castro; K. Luengas-Villamil; C. Vergara; J.A. Rodriguez; J.M. Shostell
10/06/2008
meat quality; Pig; RH mapping

The suppressive subtractive hybridization technique was previously used by the authors to identify candidate genes for meat quality in pig. A set of ESTs homologous (>95%) to genes involved in muscle metabolism is reported in the present paper. Four ESTs homologous to MYH1, KALRN, MLC2V, and SNX13 genes plus two genes (AK1, PPIA) used as housekeeping for muscle tissue were assigned to ... more

C. Gorni; S. Iacuaniello; B. Castiglioni; G. Pagnacco; P. Mariani
12/08/2011
Alternative splicing variant; DRD2 gene; Expression; Pig; Polymorphism

The dopamine D2 receptor (DRD2) is a crucial mediator for normal physiological processes. We cloned the pig DRD2 gene, investigated its distribution in tissues and identified polymorphisms by RT-PCR, quantitative real-time PCR and direct sequencing. Two Yorkshire pigs from Guangdong Academy of Agricultural Sciences (Guangzhou, China) were selected to clone the gene and ... more

H.P. Xu; X.M. He; M.X. Fang; Y.S. Hu; X.Z. Jia; Q.H. Nie; X.Q. Zhang
09/04/2013
Glycogenin-1; Glycolytic potential; Muscle-fiber type; Myosin heavy chains; Pig

This study analyzed the effect of muscle-fiber type composition on glycogenin-1 (GYG) gene expression and its impact on pH. The longissimus dorsi (LD) muscle contains more type IIB fibers (75.10%) than does the psoas major (PM) muscle (41.58%), while the PM has more type I (3.65 vs 0.94%), type IIA (34.15 vs 10.63%), and type IIX (20.62 vs 13.33%) fibers. ... more

S.H. Zhang; L. Zhu; Z.H. Wu; Y. Zhang; G.Q. Tang; Y.Z. Jiang; M.Z. Li; L. Bai; X.W. Li
07/30/2013
Escherichia coli F18; Pig; Real-time PCR; TLR4 gene

TLR4 is the main recognition receptor of bacterial lipopolysaccharides, which play an important role in innate and adaptive immunity. We used real-time PCR to analyze the tissue expression profile and differential expression of TLR4 in 4 pig populations (Escherichia coli F18-resistant Sutai, E. coli F18-sensitive Sutai, Large White, Meishan), in order ... more

J. Wang; Z.Y. Pan; X.R. Zheng; Z.C. Wu; X.M. Su; G.Q. Zhu; X.G. Huang; S.L. Wu; W.B. Bao
06/28/2013
BPI gene; Gene expression; Pig; Real-time PCR

We compared and analyzed the expression of the BPI gene of Sutai piglets ranging from newborn to post-weaning days 8, 18, 30, and 35 by the real-time PCR method, in order to determine if it is involved in protection against disease caused by ETEC F18. There was a significant difference between 18 and 35-day expres­sion in the jejunum. There were also significant differences ... more

J. Zhu; C. Zi; Z.C. Wu; L. Liu; X.R. Zheng; X.M. Su; G.Q. Zhu; X.G. Huang; S.L. Wu; W.B. Bao
09/26/2012
DNA methylation; Fat; MSAP; Muscle; Pig

DNA methylation may be involved in regulating the expression of protein-coding genes, resulting in different fat and muscle phenotypes. Using a methylation-sensitive amplified polymorphism approach, we obtained 7423 bands by selective amplification of genomic DNA from six different fat depots and two heterogeneous muscle types from Duroc/Landrace/Yorkshire cross-bred pigs. The degrees of ... more

J.D. Ma; M.Z. Li; S.L. Zhou; C.W. Zhou; X.W. Li
08/16/2012
Milk; miRNA; Normalization; Pig; Quantitative PCR

Determination of an optimal set/number of internal control microRNA (miRNA) genes is a critical, but often undervalued, detail of quantitative gene expression analysis. No validated internal genes for miRNA quantitative PCR (q-PCR) in pig milk were available. We compared the expression stability of six porcine internal control miRNA genes in pig milk from different lactation periods (1 h ... more

Y.R. Gu; Y. Liang; J.J. Gong; K. Zeng; Z.Q. Li; Y.F. Lei; Z.P. He; X.B. Lv

Pages