Research Article

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07/19/2011
5' Anchored PCR; Larimichthys polyactis; Microsatellite markers; Polymorphism; Redlip croaker

Larimichthys polyactis is a commercially important marine fish species in southeast Asia. The population crashed due to overfishing in the 1970s, but has since recovered. We developed 13 novel polymorphic microsatellite markers in L. polyactis using 5' anchored PCR. The characteristics of these loci were estimated by analyzing a sample of 30 individuals. A total of 74 ... more

C.Y. Ma; H.Y. Ma; L.B. Ma
03/15/2011
Beetle; Brontispa longissima; Microsatellite markers; Polymorphism

Brontispa longissima is one of the most serious insect pests of coconut in Southeast Asia; it was first discovered on Hainan Island in June 2002. Despite the economic risk associated with this pest, genetic aspects of the invasion process have remained relatively unexplored. Using microsatellite markers, we investigated the population structure, genetic variability and pattern of ... more

C.L. Ma; H.L. Wu; H.Y. Hu; X. Wu; G.C. Ma; Y.G. Fu; Z.Q. Peng
05/29/2015
Cross-amplification; Microsatellite markers; Paramisgurnus dabryanus; Polymorphism

The large-scale loach, Paramisgurnus dabryanus, is a small freshwater fish of major economic importance in many Asian countries, particularly China and South Korea. Fifteen polymorphic microsatellite (simple sequence repeat) markers were obtained through cross-species amplification between this loach and a related species, Misgurnus anguillicaudatus (GenBank accession numbers: KC117456 to ... more

G. Chen; S. Huang; J. Gao; X. Bai; W. Wang; X. Cao
03/06/2015
Date palm; Genetic diversity; Microsatellite markers; Phoenix dactylifera L.; Polymorphism

The genetic diversity in the date palm germplasm of 59 female accessions representing 12 cultivars from different locations in Qatar was investigated using 14 loci of simple-sequence repeat (SSR) primers. A total of 94 alleles, with a mean of 6.7 alleles per locus, were scored. The number of alleles per locus varied from 3 (primer mPdCIR090) to 11 (primers mPdCIR010 and mPdCIR015). The ... more

K. Elmeer; I. Mattat
11/18/2013
American cattle breeds; Brazilian cattle; Genetic diversity; Microsatellite markers; Population structure

The Brazilian cattle population is mainly composed of breeds of zebuine origin and their American derivatives. Comprehensive knowledge about the genetic diversity of these populations is fundamental for animal breeding programs and the conservation of genetic resources. This study aimed to assess the phylogenetic relationships, levels of genetic diversity, and patterns of taurine/zebuine ... more

B.S.A.F. Brasil; E.G.A. Coelho; M.G. Drummond; D.A.A. Oliveira
04/10/2013
Genetic diversity; Microsatellite markers; Population genetic structure

We analyzed population structure and genetic diversity in Blanco Orejinegro Creole cattle with 12 microsatellite markers, genotyping 138 individuals belonging to 7 commercial and 3 conservation herds. These markers showed a high level of polymorphism; 171 alleles were identified. The mean number of alleles per locus was 5.63 (3.82-6.58). The total number of alleles per marker was 14.2 and ... more

R. Martínez; A.P. Llinás; J.F. M.-Rocha
12/18/2014
Breeding; Conservation; Guava; Microsatellite markers; Morphological descriptors

The family Myrtaceae is widespread in the Atlantic Forest and is well-represented in the Espírito Santo State in Brazil. In the genus Psidium of this family, guava (Psidium guajava L.) is the most economically important species. Guava is widely cultivated in tropical and subtropical countries; however, the widespread cultivation of only a small number of guava tree ... more

A.M. Nogueira; M.F.S. Ferreira; J.H.S. Guilhen; A. Ferreira
10/27/2014
Genetic diversity; Malaria vector; Microsatellite markers; Population genetics; Speciation

Anopheles nuneztovari sensu lato consists of cryptic species and genetic lineages, one of which is an important human malaria vector in the northern part of South America. Population structure and evolutionary genetics studies may help in the definition and delimitation of the species and lineages within this species complex, which is relevant information for organizations ... more

A.S. Cunha-Machado; V.M. Scarpassa
04/03/2014
Genetic diversity; Microsatellite markers; Population genetic structure; Setipinna taty

Microsatellite markers for the half-fin anchovy Setipinna taty were developed from the enriched (CA)15 genomic library, and they were used for the population genetic studies of the half-fin anchovy from Chinese coastal areas. Samples were collected from five localities of the East China Sea and the Yellow Sea. Eleven simple sequence repeat markers were used to assess ... more

Y.N. Sun; Y. Qin; Z.H. Zhu; D.Q. Sun
01/22/2014
Cross-amplification; Microsatellite markers; Population genetics; Siniperca chuatsi

The mandarin fish is a popular fresh water food fish in China. Fifty-three polymorphic microsatellite markers were isolated through construction of an enriched library of genomic DNA of Siniperca chuatsi (Percichthyidae). We found 2 to 7 alleles per locus. The observed and expected heterozygosity values varied from 0.059 to 1.000 and from 0.305 to 0.818, respectively. The ... more

C.X. Tian; X.F. Liang; M. Yang; Y.Q. Dou; H.Z. Zheng; L. Cao; Y.C. Yuan; C. Zhao

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