Research Article

Related GMR Articles

Broad-complex; Gene expression; In situ hybridization; RACE-PCR; Real-time PCR

Broad-Complex (BR-C) is an early ecdysone-responsive gene encoding a family of zinc-finger transcription factors that function during metamorphosis in insects. We identified two full-length cDNAs of BR-C Z6 in the giant tiger shrimp (Penaeus monodon). They were 2422 and 2060 bp in length, containing open reading frames of 1440 and 1443 bp, corresponding to ... more

A. Buaklin; S. Klinbunga; P. Mensveta
cDNA array; Demineralized bone matrix; Gene expression; Human periosteal cells; Osteogenesis

Demineralized bone matrix (DBM) has been widely investigated as a biomaterial to promote new bone formation and is utilized clinically for bone repair and regeneration. We investigated gene expression patterns of osteogenic differentiation in human periosteal (HPO) cells cultured with demineralized bone matrix, using cDNA array technology. Osteogenic differentiation of HPO cells was ... more

S. Honsawek; P. Bumrungpanichthaworn; T. Thitiset; L. Wolfinbarger
Adipocyte fatty acid-binding protein; Gene expression; Longissimus dorsi; meat quality; Pigs; Polymorphism

Recent attention in pig breeding programs has focused on the improvement of pork quality in response to increasing consumer demands. Compared to the fatty-type Northeastern Indigenous (Chinese) breed of pigs, the lean-type Large White has lower intramuscular fat and inferior eating quality from the perspective of the Chinese consumer. In order to investigate the molecular basis of ... more

Y. Gao; Y.H. Zhang; H. Jiang; S.Q. Xiao; S. Wang; Q. Ma; G.J. Sun; F.J. Li; Q. Deng; L.S. Dai; Z.H. Zhao; X.S. Cui; S.M. Zhang; D.F. Liu; J.B. Zhang
Cassava; Gene expression; Gene walking; promoters

Cassava can be cultivated on impoverished soils with minimum inputs, and its storage roots are a staple food for millions in Africa. However, these roots are low in bioavailable nutrients and in protein content, contain cyanogenic glycosides, and suffer from a very short post-harvest shelf-life, and the plant is susceptible to viral and bacterial diseases prevalent in Africa. The demand ... more

M.A. Gbadegesin; J.R. Beeching
Gene expression; Genetic variability; Isoenzymes; Ontogenetic stages

Sib-seedlings of 95 strains of the strictly autogamous grass Hordeum euclaston were analyzed by horizontal polyacrylamide gel electrophoresis for four isoenzyme systems at a specific ontogenetic stage. We found differences in the activity of some genes among individuals of this species. Hence, an ontogenetic analysis was carried out to investigate 12 strains at five ontogenetic ... more

J.E. Georg-Kraemer; C.A.S. Ferreira; S.S. Cavalli
Gene expression; Partial hepatectomy; Pit cells; Rat liver regeneration

Hepatic pit cells are a population of large granular lymphocytes that substantially contribute to hepatic immunity. Studies have proven that pit cells have a role in liver regeneration, but the details of the relationship between pit cells and liver regeneration is not clear at present. We subjected rats to a two-third hepatectomy; pit cells with high purity were obtained with Percoll ... more

C.S. Xu; X.G. Chen; C.F. Chang; G.P. Wang; W.B. Wang; L.X. Zhang; Q.S. Zhu; L. Wang
Gene expression; In situ hybridization; Planarian; Regeneration; STAG

We examined the spatial and temporal expression of the planarian Dugesia japonica STAG-related gene (DjStag), in both intact and regenerating planarians, by whole-mount in situ hybridization and relative quantitative real-time PCR. The first localized transcripts of DjStag were detected in the blastemas three days after amputation, in all regenerates including those ... more

Z.Q. Yuan; B.S. Zhao; J.Y. Zhang
Cacao; Gene expression; Genetic marker; Lethal factor

The lethal gene ‘Luteus-Pa’ is found in cacao genotypes (Theobroma cacao) of the Parinari (Pa) series, from Peru. Seedlings affected by this gene have yellowing leaves and subsequently die. We mapped this gene based on microsatellite markers and RAPDs, in order to elucidate the inheritance of ‘Luteus-Pa’ and investigate possible lethal mechanisms. DNA samples of ... more

B.C. Rehem; A.A.F. Almeida; R.X. Corrêa; A.S. Gesteira; M.M. Yamada; R.R. Valle
Dreb gene; Drought; Gene expression; Glycine max; Quantitative PCR; Water deficit

We determined the expression levels of DREB transcription factor (Gmdreb1) and of the genes Gmgols, Gmpip1b, Gmereb, and Gmdefensin in drought-tolerant (MG/BR46-Conquista) and drought-sensitive (BR16) genotypes of soybean, during drought. The trial was carried out in a controlled-environment chamber, set up to provide drought conditions. ... more

R. Stolf-Moreira; M.E. Medri; N. Neumaier; N.G. Lemos; R.L. Brogin; F.C. Marcelino; M.C.N. de Oliveira; J.R.B. Farias; R.V. Abdelnoor; A.L. Nepomuceno
Bovine; Calcium; Gene expression; Kidney

In order to better understand vitamin D3 in cattle metabolism, we quantified 1alpha-HYD and 24-HYD gene expression. In the kidneys of 35 male Nellore cattle, these were divided into a control group and two treatment groups (2 x 106 international units of vitamin D3 administered for 2 or 8 consecutive days pre-slaughter). Vitamin D3 ... more

L.R. Rezende; E.F. Delgado; A.R.L. Júnior; G. Gasparin; E.C. Jorge; G.B. Mourão; L.L. Coutinho