Research Article

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10/18/2013
Anticancer activity; cDNA; Cloning; Giant panda; Overexpression; Purification; Ribosomal protein L24

The ribosomal protein L24 (RPL24) belongs to the L24E family of ribosomal proteins and is located in the cytoplasm. The purpose of this study was to investigate the structure and anti-cancer function of RPL24 of the giant panda (Ailuropoda melanoleuca). The complementary DNA of RPL24 was cloned successfully using reverse transcription-polymerase chain reaction technology ... more

Y.L. Hou; X. Ding; W. Hou; B. Song; T. Wang; F. Wang; J. Li; J. Zhong; T. Xu; B.X. Ma; H.Q. Zhu; J.H. Li; J.C. Zhong
04/10/2013
Anthocephalus chinensis; Gene cloning; Phylogenetic tree; α-expansin

Expansins are cell wall-associated proteins that induce wall extension and relax stress by disrupting noncovalent bonds between cellulose microfibrils and cross-linking glycan chains, thereby promoting wall creep. Anthocephalus chinensis is a very fast-growing economically important tree found mainly in South Asia. Sixteen cDNAs, designated AcEXPA1 to AcEXPA16 ( ... more

K.X. Ouyang; M.Q. Liu; R.Q. Pian; S.S. Liu; X.Y. Chen
01/09/2012
Calmodulin; Gene cloning; Hyriopsis schlegelii; Nacre; Real-time polymerase chain reaction

Calmodulin (CaM) is a multifunctional intracellular calcium ion receptor protein that participates in a range of cellular processes, including calcium metabolism in mussels. To investigate the role of CaM in freshwater mollusk shell calcium metabolism, the full-length CaM cDNA was isolated from the freshwater pearl mussel, Hyriopsis schlegelii (referred to as hsCaM) ... more

L.G. Zeng; J.H. Wang; Y.J. Li; J.Q. Sheng; Q. Gu; Y.J. Hong
02/13/2014
DDRT; Gene cloning; Glycine max; Heterosis; Soybean

Here, we compare the molecular mechanism of soybean heterosis through the differential expression of basic cloning. Specifically, we cloned 22 differentially expressed cDNA fragments from hybrid combinations of Jilin 38 x EXP (which had obvious yield advantages) and their parents. In addition, we compared the homology of these fragments and predicted their functions. Cloning ... more

J. Zhang; D. Yao; P. Wang; S.Y. Guan; J. Ma; Y.P. Fu
07/04/2014
3-glucan binding protein; Gene cloning; Hard clam; Lipopolysaccharide and β-1; Meretrix meretrix; mRNA expression

Pattern recognition molecules play an important role in innate immunity by recognizing conserved molecular patterns that are present on the surface of invading microorganisms. In this study, a lipopolysaccharide and β-1,3-glucan binding protein (LGBP) gene was cloned from the hard clam Meretrix meretrix (designated as Mm-LGBP) by the expressed sequence tags and ... more

S.X. Liu; Z.H. Qi; J.J. Zhang; C.B. He; X.G. Gao; H.J. Li
03/17/2014
Capsicum annuum L.; Gene cloning; Gene expression; HsfA2

The heat-shock transcription factor (Hsf) gene CaHsfA2 (GenBank accession No. JX402923) was cloned from the Capsicum annuum thermotolerant line R9 by combining the techniques electron cloning and rapid amplification of cDNA ends. The gene, which is 1436 bp in length, had an open reading frame of 1089 bp that encoded 362 amino acids. There was an 831-bp intron between ... more

M. Guo; Y.X. Yin; J.J. Ji; B.P. Ma; M.H. Lu; Z.H. Gong
05/29/2015
Cold shock; Embryonic development; Gene cloning; mRNA expression; Sepiella maindroni (SmYB); Y-box

Y-box proteins are a family of highly conserved nucleic acid binding proteins that interact with genome and transcription product to modulate the transcriptional and translational processes. In the present study, a complete mRNA of Y-box binding protein (designated SmYB) was obtained from Sepiella maindroni by amplification of flanking sequences. The full size of SmYB cDNA was ... more

H.P. Si; C. .L.Wang; Y.Y. Zhang; C.K. Mu; P.P. Zhan; R.H. Li; W.W. Song
04/30/2015
Female sterile homeotic (fsh); Gene cloning; Gene expression; Macrobrachium nipponense

The gene female sterile homeotic (fsh) plays crucial roles in molecular function, including protein kinase activity and DNA binding, which are involved in biological processes such as terminal region determination and negative regulation of DNA-dependent transcription. Although fsh has been found in Drosophila melanogaster, little is known regarding its ... more

W.Y. Zhang; S.F. Jiang; Y.W. Xiong; H.T. Fu; H. Qiao; S.M. Sun; Y.S. Gong; S.B. Jin
09/30/2007
genetic relationships; Groundnut; Microsatellites; peanut; Simple sequence repeat

The Kayabi Indians who inhabit the Xingu Indigenous Park, located in West Central Brazil, have grown and managed peanuts for a long time. A great number of landraces are being maintained by these tribes and some of this germplasm has morphological traits that exceed the variation described in the taxonomic literature. Here, we analyzed the genetic variability of these landraces using a ... more

F.O. Freitas; M.C. Moretzsohn; J.F.M. Valls
03/17/2014
3-glucanase gene; Arachis hypogaea L.; Disease resistance; Genetic transformation; peanut; Vector construction; β-1

Plant β-1,3-glucanases are commonly involved in disease resistance. This report describes the cloning and genetic transformation of a β-1,3-glucanase gene from peanut. The gene was isolated from both the genomic DNA and cDNA of peanut variety Huayu20 by polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR), respectively. The DNA sequence contained 1471 bp including two ... more

L.X. Qiao; X. Ding; H.C. Wang; J.M. Sui; J.S. Wang

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