Research Article

Related GMR Articles

Aneuploidy; Prenatal diagnosis; QF-PCR

Quantitative fluorescent polymerase chain reaction (QF-PCR) is an accurate and reliable method for rapid detection of aneuploidy; however, it is not routinely used in China. We aimed to validate QF-PCR as a means for prenatal common aneuploidy screening and to analyze the heterozygosities of short tandem repeat (STR) markers in the Chinese population. The sequences of 19 STR markers in ... more

A.Q. Xu; M. Xia; J.T. Liu; F.X. Yao; W.M. Zhang; N. Hao; J. Zhou; X.M. Bian
Adventitious embryony; Aneuploidy; Apospory; Hybridization; Polyploidy; Sterility

Cassava is the most important staple crop in the Tropics and Subtropics. Apomixis may revolutionize its production due to various attributes. These potential advantages include production by true seed, maintaining cultivar superiority over generations without segregation, and avoiding contamination by bacteria and viruses. Historically, apomixis was initially observed by International ... more

D.Y.H. Freitas; N.M.A. Nassar
Aneuploidy; Apomixis; interspecific hybrid; Wild species

An interspecific hybrid between cassava and Manihot glaziovii acquired an apomixis gene from the parent M. glaziovii. This hybrid was exposed to open pollination during three subsequent generations. Seven sibs and the maternal progenitor of the fourth generation were genotyped using six microsatellite loci previously developed for cassava. All sibs were identical with ... more

N.M.A. Nassar; P.T.C. Gomes; A.M. Chaib; N.N. Bomfim; R.C.D. Batista; R.G. Collevatti
Bladder cancer; Gene polymorphism; Meta-analysis; p53 codon 72

Although there have been many studies investigating a possible association between p53 codon 72 polymorphism and risk of bladder cancer, the results have been inconsistent. We conducted a meta-analysis of six epidemiological studies, which included 597 bladder cancer cases and 731 controls. Patients with bladder cancer had a significantly lower frequency of Pro/Arg [odds ratio (OR) = 0.80 ... more

D.B. Li; X. Wei; L.H. Jiang; Y. Wang; F. Xu
Bladder cancer; CCR2-V64I; Chemokines; MCP-1 A-2518G; Polymorphism

Chemokines are potent proinflammatory cytokines that are implicated in numerous inflammatory diseases. Proinflammatory gene polymorphisms lead to variations in the production and concentration of inflammatory proteins. We investigated a possible association between polymorphisms in chemokine and chemokine receptor genes (MCP-1 A-2518G and CCR2-V64I) and bladder cancer risk. Genotypes were ... more

K.F. Narter; B. Agachan; S. Sozen; Z.B. Cincin; T. Isbir
Bladder cancer; KEGG pathway; T1-T2; Ta-T1; Tumor grades

Bladder cancer is a highly heterogeneous neoplasm. We examined the gene expression profile in 3 bladder cancer stages (Ta, T1, T2) using expression microarray analysis of 40 bladder tumors. Differentially expressed genes were found by the t-test, with <0.005 as the significance threshold. KEGG pathway-enrichment analysis was used to study the signaling pathways of the genes. ... more

Z.Q. Fang; W.D. Zang; R. Chen; B.W. Ye; X.W. Wang; S.H. Yi; W. Chen; F. He; G. Ye
Bladder cancer; Recurrence; Survivin; Vascular endothelial growth factor

Survivin and vascular endothelial growth factor (VEGF) are newly discovered tumor markers closely correlated with bladder cancer. We analyzed the expression of survivin and VEGF in paraffin-embedded tumor tissues from 78 patients with bladder transitional cell carcinoma (BTCC) using an immunohistochemistry method. Normal bladder mucosae from 10 non-BTCC cases were also included as a ... more

Y.W. Sun; Q. Xuan; Q.A. Shu; S.S. Wu; H. Chen; J. Xiao; P. Xiang; Y.P. Zhu; F.L. Wang; S.T. Zhao
Apoptosis; Bladder cancer; Gene expression; RT-PCR

We examined the expression of anti-apoptotic genes (XIAP and Bcl-2) and apoptotic genes (cytochrome c, caspase-9, Apaf-1) in tissue samples of patients with superficial bladder cancer. Thirty-two bladder cancer tissue samples (8 papillary urothelial neoplasm of low malignant potential, 10 low-grade, and 14 high-grade) and 8 normal bladder ... more

D.F. Barione; F.S.N. Lizarte; P.C. Novais; C.A.M. de Carvalho; F.C.B. Valeri; F.M. Peria; H.F. de Oliveira; D.L. Zanette; W.A. Silva; A.J. Cologna; R.B. Reis; S. Tucci; A.C.P. Martins; D.P.C. Tirapelli; L.F. Tirapelli
Bladder cancer; Genetic susceptibility; hOGG1; Meta-analysis; Polymorphism

The functional polymorphism Ser326Cys (rs1052133) in the human 8-oxoguanine DNA glycosylase (hOGG1) gene has been implicated in bladder cancer risk. However, reports of this association between the Ser326Cys polymorphism and bladder cancer risk are conflicting. In order to help clarify this relationship, we made a meta-analysis of seven case-control studies, summing 2521 cases ... more

D.Y. Zhong; H.Y. Chu; M.L. Wang; L. Ma; D.N. Shi; Z.D. Zhang
Apoptosis; Biological behavior; Bladder cancer; Enhancer of zeste homolog 2; siRNA

We investigated the effect of siRNA targeting enhancer of EZH2 on cell proliferation, invasion, migration, and apoptosis of human bladder cancer T24 cells. An siRNA-expressing plasmid targeting the EZH2 gene was transfected into T24 cells. Quantitative polymerase chain reaction and Western blot analysis were used to detect EZH2 expression at the mRNA and protein levels, ... more

H.F. Wang; H. Yang; L.B. Hu; Y.H. Lei; Y. Qin; J. Li; C.W. Bi; J.S. Wang; Q. Huo