Research Article

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Recent attention in pig breeding programs has focused on the improvement of pork quality in response to increasing consumer demands. Compared to the fatty-type Northeastern Indigenous (Chinese) breed of pigs, the lean-type Large White has lower intramuscular fat and inferior eating quality from the perspective of the Chinese consumer. In order to investigate the molecular basis of ... more

Y. Gao; Y.H. Zhang; H. Jiang; S.Q. Xiao; S. Wang; Q. Ma; G.J. Sun; F.J. Li; Q. Deng; L.S. Dai; Z.H. Zhao; X.S. Cui; S.M. Zhang; D.F. Liu; J.B. Zhang
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Evodiamine is the main active alkali of Wu Zhuyu, a widely distributed Chinese herb. It plays an important role in the regulation of body fat deposition. In this study, we aimed to investigate the effect of evodiamine administration on the expression of genes involved in lipid metabolism in the liver and adipose tissue. Fasted mice were subcutaneously injected with evodiamine (37°C, 20 ... more

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The aim of this study was to investigate the effects of partial hepatic ischemia/reperfusion (I/R) on postoperative cognitive function in mice. One hundred Kunming mice were randomized into control group (N = 20), sham group (N = 20) and I/R group (N = 60), which was equally divided into 3 subgroups according to the ischemia time (20, 30 and 40 min). Half of the mice in each group ... more

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Cattle; Effective population size; Inbreeding; Pigs; Sheep; Software

Genetic variation provides a basis upon which populations can be genetically improved. Management of animal genetic resources in order to minimize loss of genetic diversity both within and across breeds has recently received attention at different levels, e.g., breed, national and international levels. A major need for sustainable improvement and conservation programs is accurate ... more

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Pigs; RNA purification; Slaughterhouse

Since RNA extraction is a crucial step in many molecular techniques, the protocols for sample collection and RNA purification need to be adapted to optimize their performance when samples are collected from animals at commercial facilities. Here we provide an RNA purification protocol for animal tissues collected from slaughterhouses. This protocol, modified from other techniques, uses ... more

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Free amino acids; Gene expression; performance; Pigs; Protein-bound

Free amino acids (AA) appear to be absorbed faster than protein-bound AA (PB-AA). We conducted an experiment to assess the effect of feeding pigs with a partially free (F-AA) or totally PB-AA diet on expression of selected genes and performance of pigs. The expression of cationic AA transporters b0,+ and CAT-1 in intestinal mucosa, liver, and longissimus (LM) and ... more

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ANGPTL4; Association analysis; Differential expression; Intramuscular fat; Pigs

In a previous study, we investigated differences in gene expression in backfat between Meishan and Large White pigs and their F1 hybrids, Large White x Meishan, and Meishan x Large White pigs. One potential differentially expressed sequence tag from the mRNA differential display was a homolog of the human angiopoietin-like 4 (ANGPTL4) gene, which encodes a protein that ... more

Z.Q. Ren; W.J. Wu; W.H. Liu; R. Zheng; J.L. Li; B. Zuo; D.Q. Xu; F.E. Li; M.G. Lei; D.B. Ni; Y.Z. Xiong
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AS-PCR (allele-specific PCR); IGFBP-5 gene; Pigs; SNP

The genetic resources and the mechanism of miniaturization in the Tibet Mini-pig have not been comprehensively studied. Polymorphisms in genes related to the insulin-like growth factor (IGF) axis have been investigated for years, but few on the polymorphism of IGF-binding protein-5 (IGFBP-5) in the Tibetan pig. In this study, allele-specific polymerase chain reaction (AS-PCR) was used to ... more

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Gene expression; Leu:Lys ratio; Pigs; Serum amino acids

We examined 96 pigs (28.1 ± 0.83 kg) to analyze the effect of Leu:Lys ratios on expression of the cationic amino acid transporters b0,+ and CAT-1 in the jejunum and liver as well as myosin expression in 2 muscles to estimate the optimum standardized ileal digestible (SID) Leu:Lys ratio for growth rate and efficiency. A wheat-and wheat bran-based diets were formulated to meet ... more

H. García; A. Morales; A. Araiza; J.K. Htoo; M. Cervantes
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Euglena gracilis; Reverse transcription-polymerase chain reaction; RNA isolation; Sodium dodecyl sulfate-Tris

RNA isolation is essential to the study of gene expression at the molecular level. However, it is difficult to isolate RNA from organisms that contain large amounts of polysaccharides or other compounds that bind or coprecipitate with RNA, such as the unicellular protist Euglena gracilis. Currently, there is no commercial kit available that is specific for the isolation of high- ... more

D. González-Mendoza; A. Morales-Trejo; H. Brito-Vera

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