Research Article

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10/04/2012
Alternative splicing; Cancer-testis gene; Cloning; ODF3; ODF4

The outer dense fiber (ODF) genes encode proteins that co-assemble along the axoneme of the sperm tail. Recently, it was demonstrated that some ODF genes are aberrantly expressed in tumors, including prostate adenocarcinoma, basal cell carcinoma, and chronic myeloid lymphoma. We cloned ODF3 and ODF4 cDNA from the testis of a patient suffering from ... more

S. Ghafouri-Fard; S. Ghafouri-Fard; M.H. Modarressi
08/29/2012
BAC library; Bubalus bubalis; Cloning; Pulse-field gel electrophoresis

River buffalo genome analyses have advanced significantly in the last decade, and the genome sequence of Bubalus bubalis will be available shortly. Nonetheless, large-insert DNA library resources such as bacterial artificial chromosomes (BAC) are still required for validation and accurate assembly of the genome sequence. We constructed a river buffalo BAC library containing 52, ... more

N.B. Stafuzza; C.A. Abbey; C.A. Gill; J.E. Womack; M.E.J. Amaral
08/29/2012
Capsicum annuum L.; Cloning; Cytoplasmic male sterility; NADP-isocitrate dehydrogenase; Sequence characterization

NADP-dependent isocitrate dehydrogenase (NADP-ICDH) is an important enzyme involved in energy metabolism. The complete coding sequence of the pepper (Capsicum annuum) NADP-ICDH gene was amplified using a reverse transcriptase PCR based on the conserved sequence information of the tomato and other Solanaceae plants and known highly homologous pepper ESTs. ... more

M.H. Deng; J.F. Wen; J.L. Huo; H.S. Zhu; X.Z. Dai; Z.Q. Zhang; H. Zhou; X.X. Zou
06/15/2012
C/EBPα gene; Cloning; Expression profile; Protein structure prediction; Qinchuan cattle

CCAAT/enhancer-binding protein alpha (C/EBPα) is an essential transcription factor, regulating the differentiation of adipocytes. We cloned the complete open reading frame of C/EBPα gene of Qinchuan cattle and analyzed its protein structures and expression profile in 15 tissues via DNA cloning, sequencing and RT-PCR. Analysis of the putative protein sequences revealed that C/EBPα ... more

H. Wang; L.S. Zan; H.B. Wang; C. Gong; C.Z. Fu
12/18/2014
Bioinformatic analysis; Calcium-dependent protein kinases; Cloning; TgCDPK6; Toxoplasma gondii

Toxoplasma gondii is recognized as an opportunistic human pathogen with a worldwide distribution. Development of effective vaccines is considered the only ideal way to control T. gondii infection. However, only one live vaccine is commercially available for use in sheep and goats. Therefore, the identification of more effective antigenic proteins is very ... more

N.Z. Zhang; S.Y. Huang; D.H. Zhou; Y. Xu; J.J. He; X.Q. Zhu
12/04/2014
Cloning; Date palm; Maturase K (matK); Ribulose bisphosphate carboxylase (rbcL); Sequencing; Taxonomy

DNA barcoding is a promising tool for species identification at the molecular level. The barcoding system is well established for species differentiation in animals, while it is less common in plants. We evaluated 2 barcoding regions, maturase K (matK) and ribulose bisphosphate carboxylase (rbcL), to compare species of Palmae according to amplification success, ... more

A. Naeem; A.A. Khan; H.M.N. Cheema; I.A. Khan; A. Buerkert
12/04/2014
Anthocyanin; Cloning; Coloration; Gene expression; Peach flower

To elucidate the connection between flower coloration and the expression of genes associated with anthocyanin biosynthesis, a gene encoding UDP-glucose: flavonoid 3-O-glucosyltransferase (UFGT) was isolated, and the expression of the last four genes in the anthocyanin biosynthetic pathway during peach flower development was determined. The nucleotide sequence of the peach ... more

X.C. Wen; J. Han; X.P. Leng; R.J. Ma; W.B. Jiang; J.G. Fang
07/04/2014
Actin gene; Aloe (Aloe spp); Cloning; RNA extraction

Aloe (Aloe spp), containing abundant polysaccharides and numerous bioactive ingredients, has remarkable medical, ornamental, calleidic, and edible values. In the present study, the total RNA was extracted from aloe leaf tissue. The isolated high-quality RNA was further used to clone actin gene by using reverse transcription-polymerase chain reaction (RT-PCR). The result of ... more

S.S. Wen; D.W. He; C.M. Liao; J. Li; G.Q. Wen; X.H. Liu
05/23/2014
Cloning; Distribution; Isoforms; Macrobrachium nipponense; Retinoid X receptor

Retinoid X receptors (RXR) are members of the nuclear receptor family that are conserved from invertebrates to vertebrates, and they play an essential role in regulating reproductive maturation, molting, and embryo development. In this study, five RXR isoforms, named RXRL2 (L, long form), RXRL3, RXRS1 (S, short form), RXRS2, and RXRS3, containing six domains from A to F, were cloned from ... more

Z. Li; W.Q. Wang; E.F. Zhang; G.F. Qiu
05/30/2014
Cloning; RAPD; Rose; SCAR; Sequencing

Roses (Rosa indica) belong to one of the most crucial groups of plants in the floriculture industry. Rosa species have special fragrances of interest to the perfume and pharmaceutical industries. The genetic diversity of plants based on morphological characteristics is difficult to measure under natural conditions due to the influence of environmental factors, which is ... more

K.M.I. Bashir; F.S. Awan; I.A. Khan; A.I. Khan; M. Usman

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