Research Article

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DNA; Indels; Insertion-deletion polymorphisms; Paternity testing; Single nucleotide polymorphisms

We developed a panel of 40 multiplexed short insertion-deletion (indel) polymorphic loci with widespread chromosomal locations and allele frequencies close to 0.50 in the European population. We genotyped these markers in 360 unrelated self-classified White Brazilians and 50 mother-child-probable father trios with proven paternity. The average heterozygosity (gene diversity) per locus was ... more

J.R. Pimenta; S.D.J. Pena
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DNA; Minisequencing; Multiplex; Polymorphisms; Single nucleotide polymorphisms

Several technologically sophisticated high-throughput techniques have been recently developed for the study of human single nucleotide polymorphisms and the diagnosis of point mutations in human diseases. However, there is also a need for simple and inexpensive techniques suitable for clinical services and small research laboratories. Minisequencing meets the latter requirements. It is ... more

C.M.B. Carvalho; S.D.J. Pena
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Breeding; Corn silage; Male sterility; Meiotic mutation; ms17

Cytological analysis under light microscopy of the single hybrid P30R50 of silage corn revealed an abnormal pattern of microsporogenesis that affected the meiotic products. Meiosis progressed normally until diakinesis, but before migration to the metaphase plate, bivalents underwent total desynapsis and 20 univalent chromosomes were scattered in the cytoplasm. At this stage, meiocytes ... more

M.S. Pagliarini; V.F. Souza; N. Silva; C.A. Scapim; M. Rodovalho; M.V. Faria
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Brachiaria; Breeding; Forage grass; Hybridization; Meiosis

Brachiaria humidicola is a grass adapted to seasonally swampy grasslands in Africa; two cultivars, ‘common’ and Llanero, are widely used in Brazilian pastures. New cultivars are in great demand in order to diversify current production systems to achieve improved quality and yield. Cytological analyses of 55 accessions of this species available from the Embrapa Beef Cattle ... more

K.R. Boldrini; E.V. Adamowski; N. Silva; M.S. Pagliarini; C.B. Valle
10/19/2010
Breeding; Hybrids; Meiosis; Silage corn

In breeding programs, commercial hybrids are frequently used as a source of inbred lines to obtain new hybrids. Considering that maize production is dependent on viable gametes, the selection of populations to obtain inbred lines with high meiotic stability could contribute to the formation of new silage corn hybrids adapted to specific region. We evaluated the meiotic stability of five ... more

V.F. Souza; M.S. Pagliarini; C.A. Scapim; M. Rodovalho; M.V. Faria
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Breeding; Carica papaya L.; Genetic resources; Genetic variability; Gower algorithm

We examined the genetic diversity of papaya (Carica papaya) based on morpho-agronomic and molecular data. Twenty-seven genotypes grown in Brazil were analyzed with 11 AFLP primer combinations, 23 ISSR markers, 22 qualitative, and 30 quantitative descriptors. For the joint analyses, we used the Gower algorithm (Joint Gower) and the average value of the individual dissimilarity ... more

O.N. de Jesus; J.P.X. de Freitas; J.L.L. Dantas; E.J. de Oliveira
02/06/2013
Breeding; Genetic diversity; Phaseolus vulgaris; SSR markers

The identification of germplasm genetic variability in breeding programs of the common bean (Phaseolus vulgaris) is essential for determining the potential of each combination of parent plants to obtain superior genotypes. The present study aimed to estimate the extent of genetic diversity in 172 lines and cultivars of the common bean by integrating five tests of value for ... more

P.C.B. Cardoso; M.M. Veiga; I.P.P. de Menezes; P.A.M.R. Valdisser; T.C.O. Borba; L.C. Melo; M.J. Del Peloso; C. Brondani; R.P. Vianello
06/28/2013
BmNPV; Breeding; Germplasm bank; RAPD molecular genetic markers

Brazil has only one public genetic pool of Bombyx mori strains, which was established in 2005 at Universidade Estadual de Maringá, Maringá, Paraná State. This genetic bank has been maintained, and the strains have been characterized using genetic and morphological tools. The quantitative and qualitative traits, directly or indirectly related to productivity, were evaluated in 14 ... more

N.C. Pereira; R.E.F. Munhoz; T.S. Bignotto; R. Bespalhuk; L.B. Garay; C.R.N. Saez; V.A. Fassina; A. Nembri; M.A. Fernandez
01/24/2013
Breeding; Fibroblast; Growth; QTL; SNP

In the past, the focus of broiler breeding programs on yield and carcass traits improvement led to problems related to meat quality. Awareness of public concern for quality resulted in inclusion of meat quality traits in the evaluation process. Nevertheless, few genes associated with meat quality attributes are known. Previous studies mapped quantitative trait loci for weight at 35 and 42 ... more

A.M. Felício; C. Boschiero; J.C.C. Balieiro; M.C. Ledur; J.B.S. Ferraz; A.S.A.M.T. Moura; L.L. Coutinho
05/15/2012
Brachiaria brizantha; Breeding; Forage grass; Microsporogenesis

The genus Brachiaria comprises more than 100 species and is the single most important genus of forage grass in the tropics. Brachiaria brizantha, widely used in Brazilian pastures for beef and dairy production, is native to tropical Africa. As a subsidy to the breeding program underway in Brazil, cytological studies were employed to determine the chromosome number and to ... more

M.S. Pagliarini; C.B. Valle; E.M. Santos; D.V. Mendes; Z.H. Bernardo; A.B. Mendes-Bonato; N. Silva; V. Calisto

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