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Genetic diversity; Genetic structure; Haliotis discus hannai; Microsatellites; Pacific abalone

Populations of the Pacific abalone, Haliotis discus hannai, have been severely overexploited over the past few decades in Korea. Information regarding the levels of genetic variability and structure within populations is insufficient for the development of effective strategies for conservation of genetic diversity of this species. To assess the genetic status of this species, we ... more

H.S. An; J.W. Lee; J.Y. Park
Aristaeomorpha foliacea; FIASCO; Giant red shrimp; Microsatellites

Fourteen microsatellite markers were isolated from the giant red shrimp Aristaeomorpha foliacea (Risso, 1827) using the FIASCO protocol (fast isolation by AFLP of sequences containing repeats). Polymorphism was assessed in 30 individuals from two localities of the western Mediterranean basin (N = 20 from Sardinia and N = 10 from Sicily); nine loci showed polymorphism with 2 to 19 ... more

R. Cannas; S. Marcias; F. Sacco; A. Cau; A.M. Deiana
Cerrado; Climatic change; Geographic range shift; Microsatellites

Many species are expected to suffer strong shifts in their geographic ranges due to climate changes in the next 50 years, with severe consequences for biodiversity patterns and population structure. We used here an ensemble forecast approach for obtaining species’ range in which multiple species distribution models and climatic models were combined to model loss of genetic variability in ... more

J.A.F. Diniz-Filho; R.G. Collevatti; L.J. Chaves; T.N. Soares; J.C. Nabout; T.F. Rangel; D.B. Melo; J.S. Lima; M.P.C. Telles
Chicken; EST; Gallus gallus; Genomics; Microsatellites; SSR

Many years of domestication and breeding have given rise to the wide range of chicken breeds that exist today; however, an increasing number of local chicken breeds are under threat of extinction. A comprehensive characterization of chicken markers (especially type I markers) is needed to monitor and conserve genetic diversity in this species. The explosion of genomics and functional ... more

M.R. Bakhtiarizadeh; B. Arefnejad; E. Ebrahimie; M. Ebrahimi
Cassava; M-AFLP; Manihot species; Microsatellites

We developed molecular markers for cassava based on the microsatellite-amplified fragment length polymorphism (M-AFLP) technique. Twenty primer pairs were developed and used for the analysis of 48 samples of Manihot species, consisting of M. esculenta (33), M. esculenta ssp flabellifolia (3), M. chlorosticta (3), M. carthaginensis (3 ... more

S. Whankaew; S. Sraphet; R. Thaikert; D.R. Smith; K. Triwitayakorn
Cowpea; Genetic diversity; Germplasm management; Microsatellites; Vigna unguiculata

Genetic diversity and phylogenetic relationships among 22 local cowpea (Vigna unguiculata) varieties and inbred lines collected throughout Senegal were evaluated using simple sequence repeat molecular markers. A set of 49 primer combinations were developed from cowpea genomic/expressed sequence tags and evaluated for their ability to detect polymorphisms among the various cowpea ... more

F.A. Badiane; B.S. Gowda; N. Cissé; D. Diouf; O. Sadio; M.P. Timko
Colossoma macropomum; Microsatellites; Multiplex panel; Tambaqui

The tambaqui, Colossoma macropomum, native to Brazil, is widely used in aquaculture systems. We developed a multiplex PCR panel for this species, comprising 12 microsatellite loci. This panel was used to genotype 73 specimens collected from Juruti, a city in the Brazilian Amazon. The mean number of alleles per locus was 8.8, the mean observed heterozygosity was 0.76, and the ... more

I.G. Hamoy; S. Santos
Amblyomma aureolatum; Enriched library; Microsatellites; Ticks

Amblyomma aureolatum (Pallas) is the main vector of the bacterium Rickettsia rickettsii, the etiological agent of Brazilian spotted fever. This disease is the most lethal human spotted fever rickettsiosis in the world. Microsatellite loci were isolated from a dinucleotide-enriched library produced from A. aureolatum sampled in Southeastern Brazil. Eight ... more

M. Ogrzewalska; M.M. Bajay; K. Schwarcz; S.K. Bajay; M.P.C. Telles; J.B. Pinheiro; M.I. Zucchi; A. Pinter; M.B. Labruna
Clustering; Genetic diversity; Heterotic pools; Microsatellites

The objectives of this study were to identify the population structure and to assess the genetic diversity of maize inbreds. We genotyped 81 microsatellite loci of 90 maize inbreds that were derived from tropical hybrids and populations. The population structure analysis was based on a Bayesian approach. Each subpopulation was characterized for the effective number of alleles, gene ... more

E.C.M. Lanes; J.M.S. Viana; G.P. Paes; M.F.B. Paula; C. Maia; E.T. Caixeta; G.V. Miranda
Crocodiles; Cross-species amplification; Microsatellites; Neotropical crocodiles

We tested microsatellites that were developed for the saltwater crocodile (Crocodylus porosus) for cross-species amplification and to provide an estimate of inter- and intraspecific variation among four species of Neotropical crocodiles (C. rhombifer, C. intermedius, C. acutus, and C. moreletii). Our results indicated that with the exception of ... more

A. Bashyal; B.A. Gross; M. Venegas-Anaya; F. Lowrance; L.D. Densmore