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Related GMR Articles

Conservation; Genetic diversity; Microsatellites; Pelodiscus sinensis

Twenty-one novel polymorphic microsatellite loci were isolated from the Chinese soft-shelled turtle, Pelodiscus sinensis (Trionychidae). This is a commonly consumed Asian species, especially in China, where it is reared in large numbers on farms. We screened 34 unrelated individuals from Wuhu of Anhui province in China and detected high levels of polymorphism for all 21 loci, ... more

X.J. Bu; L. Liu; L. Wang; L.W. Nie
CART gene; Goats; Microsatellites; Reproduction traits; SNPs

Polymorphisms of the CART gene were investigated by PCR-single-strand conformation polymorphism analysis in 540 samples from 10 goat breeds. Ten novel single-nucleotide polymorphisms as well as three microsatellites were detected; a mutation, 77T→C, led to an amino acid change (Leu→Ser). Associations between polymorphic loci and reproductive traits were analyzed in Chuandong White, ... more

P.Q. Wang; L.M. Deng; B.Y. Zhang; M.X. Chu; J.Z. Hou
Conservation; Genetic variation; Microsatellites; RAMs; Scleropages formosus

Malaysian arowana (dragonfish; Scleropages formosus) is an ancient osteoglossid fish from southeast Asia. Due to the high demand of the ornamental fish trade and because of habitat loss, the species is close to extinction. We isolated and characterized 10 polymorphic microsatellites of this species, using 5'-anchored PCR. The number of alleles at the 10 microsatellite loci ranged ... more

B. Manoharan; Z. Sulaimen; F. Omar; R.Y. Othman; S.Z. Mohamed; S. Bhassu
Comparative analysis of molecular groups; DNA markers; Microsatellites; RAPD and SSR loci; Zea mays

Using only one type of marker to quantify genetic diversity generates results that have been questioned in terms of reliability, when compared to the combined use of different markers. To compare the efficiency of the use of single versus multiple markers, we quantified genetic diversity among 10 S7 inbred popcorn lines using both RAPD and SSR markers, and we evaluated how well these two ... more

A.A. Leal; C.A. Mangolin; A.Tdo Amaral Júnior; L.S.A. Gonçalves; C.A. Scapim; A.S. Mott; I.B.O. Eloi; V. Cordovés; M.F.P. da Silva
Alectrurus risora; Culicivora caudacuta; Microsatellites; Tyrannidae

Tyrant flycatchers (Aves: Tyrannidae) are endemic to the New World, and many species of this group are threatened or near-threatened at the global level. The aim of this study was to test the 18 microsatellite markers that have been published for other Tyrant flycatchers in the Strange-tailed Tyrant (Alectrurus risora) and the Sharp-tailed Tyrant (Culicivora caudacuta), ... more

B. Mahler; A.R.R. Schneider; A.S. Di Giacomo; A.G. Di Giacomo; J.C. Reboreda; R. Tiedemann
Enriched library; Exopalaemon carinicauda; Microsatellites

The ridgetail white prawn Exopalaemon carinicauda is one of the major economic shrimp species cultured in China. In this study, 30 microsatellite loci were isolated and characterized for the ridgetail white prawn E. carinicauda using a microsatellite-enriched library. Polymorphisms were tested in 30 individuals from a single wild population. The number of alleles at ... more

S.W. Jia; P. Liu; J. Li; J.T. Li; L.Q. Pan
Conservation; Genetic structure; Gossypium mustelinum; Microsatellites; Wild cotton

Gossypium mustelinum is the only cotton species native to Brazil; it is endemic to the semi-arid region of the northeast. The populations are found near perennial and semi-perennial sources of water, such as ponds or pools in intermittent streams. Problems with in situ conservation derive from human interference in its habitat, mainly because of excessive cattle grazing ... more

M.F. Alves; P.A.V. Barroso; A.Y. Ciampi; L.V. Hoffmann; V.C.R. Azevedo; U. Cavalcante
454 Sequencing; Amphibians; Atlantic Forest; Euparkerella brasiliensis; Microsatellites

The new-generation 454 GS-FLX Titanium pyrosequencing was used to isolate microsatellite markers for the Brazilian Guanabara frog, Euparkerella brasiliensis, an Atlantic forest endemic species. Three multiplex polymerase chain reaction sets were optimized for genotyping of 11 polymorphic (di- and tetranucleotide) microsatellite markers. Genetic diversity was assessed in 21 ... more

L.A. Fusinatto; S. Lopes; A. Silva-Ferreira; J. Alexandrino; C.F.B. Haddad; C.F.D. Rocha; F. Sequeira
Cross-species amplification; Lake minnows; Microsatellites

Fifteen sets of PCR primers designed for the amplification of microsatellite loci from Danio rerio and Phoxinus neogaeus Campostoma DNA that have been proven applicable for molecular studies of several species of cyprinids were tested for amplification of microsatellites from lake minnow DNA. The samples were taken from 298 fish inhabiting 6 distinct populations located ... more

D. Kaczmarczyk
conservation genetics; Mauritia flexuosa; Microsatellites

Mauritia flexuosa L. (Arecaceae) is a palm tree species known as buriti that occurs in the Cerrado biome. It is characteristic of the vereda, a typical ecosystem of central Brazil. In this phytophysiognomy, M. flexuosa and other groups of arboreal-herbaceous species develop in open fields with very humid soils. M. flexuosa can be found in forest borders and is a ... more

E.V. Menezes; W.F.S. Souto; A.Y. Ciampi; V.C.R. Azevedo; H.M. Valério; M.A.S. Pimenta