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Chelex treatment; Lama guanicoe; Microsatellites; Mitochondrial control region; Non-invasive genetics; Polymerase chain reaction inhibitors

Feces-based population genetic studies have become increasingly popular. However, polymerase chain reaction (PCR) amplification rates from fecal material vary depending on the species, populations, loci, and extraction protocols. Here, we assessed the PCR amplification success of three microsatellite markers and a segment of the mitochondrial control region of DNA extracted from field- ... more

M.I. Espinosa; A. Bertin; F.A. Squeo; A. Cortés; N. Gouin
Cunninghamia lanceolata; Cupressaceae; Genetic conservation; Microsatellites; Population genetics

As a result of human activities, wild populations of Cunninghamia lanceolata (Cupressaceae) have sharply declined in recent years. The development and implementation of a valid conservation strategy require a clear understanding of the genetic makeup of this species. Eleven polymorphic microsatellite loci were isolated and characterized from samples of 52 individuals from the ... more

Y.X. Li; Z.S. Wang; J.K. Sui; Y.F. Zeng; A.G. Duan; J.G. Zhang
Microsatellites; Polymorphism; Sinonovacula constricta

The razor clam, Sinonovacula constricta, is an important commercial bivalve and a popular mollusca food in China. Twelve polymorphic microsatellite markers were isolated from the razor clam using a partial genomic library enriched for tandem repeat sequences of (CA)16, (GA)16. Polymorphisms of these loci were evaluated in a wild population of 30 individuals ... more

H.T. Ma; H.B. Jiang; X.Q. Liu; X.P. Wu; X.M. Wei
Conservation; Endangered species; Mitochondrial DNA; Oryx; Phylogenetic relationship

The present study reports the application of mitochondrial markers for the molecular phylogeny of Oryx species, including the Arabian oryx (AO), scimitar-horned oryx (SHO) and plains oryx (PO), using the Addax as an outgroup. Sequences of three molecular markers, 16S rRNA, cytochrome b and a control region, for the above four taxa were aligned and the topologies of respective ... more

H.A. Khan; I.A. Arif; A.A.Al Homaidan; A.H.Al Farhan
bottleneck; Endangered species; Microsatellite marker; Tanichthys albonubes

We developed 12 microsatellite loci for the endangered minnow species, Tanichthys albonubes, using PCR-based isolation of microsatellite arrays. These new markers were tested in 26 individuals from a wild population collected from Guangzhou in China and 26 individuals from a cultured strain. The number of alleles ranged from two to nine and the expected heterozygosity from 0.177 ... more

J. Zhao; D.Y. Tseng; H.D. Lin; X.T. Lin
Dominant marker; Endangered species; Fragmentation; Genetic structure; Tropical forest

We analyzed the genetic diversity of populations of two sympatric species of Lymania (Bromeliaceae), both endemic to the Atlantic rainforest of southern Bahia (Brazil). Lymania azurea has a restricted occurrence, while Lymania smithii has a wider distribution. Our aim was to provide genetic data to contribute to the design of more efficient conservation ... more

V.C.C. Pamponét; T.F. Alves; R.A. Martinez; R.X. Corrêa; F.A. Gaiotto
Annamocarya sinensis; Endangered species; Genetic diversity; Microsatellite; Population structure

The endangered perennial plant Annamocarya sinensis (Dode) Leroy is a tertiary relict tree restricted to southeastern China and northern Vietnam. To explore endangerment mechanisms, develop protection strategies, and guide reintroduction efforts for this species, we investigated genetic diversity and population structure by surveying 70 individuals from three distinct ... more

Z.Y. Zhang; X.M. Pang; J.W. Han; Y. Wang; Y.Y. Li
Endangered species; Genetic diversity; Mergus squamatus; Wintering habitat

The scaly-sided merganser (Mergus squamatus), found in temperate East Asia, has been reduced to a very small population. Central and southern China are its main wintering habitat. However, populations have declined greatly since the 1980s due to habitat loss and degradation, and poaching. To meet the urgent need for up-to-date conservation information, we examined RAPD DNA ... more

Z.Q. Zhang; C.J. Qin; D.A. Fang; A.P. Wang; C.H. Luo; K. Liu
Atlantic rainforest; DNA extraction protocol optimization; Endangered species; Molecular marker

Melanoxylon brauna (Fabaceae - Caesalpinioideae) is an endemic and valuable hardwood tree species in the Brazilian Atlantic rainforest; it is comparable to African ebony wood. We tested three protocols of DNA extraction based on the citrimonium bromide (CTAB) method and evaluated the quantity, purity and integrity of the DNA. We also determined whether these procedures interfere ... more

D.B. Borges; M.B. Amorim; A.M. Waldschmidt; E. Mariano-Neto; C.V. Vivas; D.G. Pereira
Cytochrome b; Endangered species; Fishery product identification; Polymerase chain reaction-restriction fragment length polymorphism

Endangered angelsharks and a protected dolphin species are illegally traded in Brazil. In this study, we determined prospective molecular markers for detecting these species in the trade of angelshark carcasses and ‘dolphin’ eyeball amulets. We compiled publicly available as well as new and unpublished cytochrome b (cyt b) DNA sequences for species involved in these ... more

L.H.O. Falcão; M.A.A. Furtado-Neto; R. Maggioni; V.V. Faria