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Linkage analysis; Microsatellites; Mystus nemurus; Pseudo-testcross strategy; River catfish; Segregation

The river catfish Mystus nemurus is an important fresh water species for aquaculture in Malaysia. We report the first genetic linkage map of M. nemurus based on segregation analysis and a linkage map using newly developed microsatellite markers of M. nemurus. A total of 70 of the newly developed polymorphic DNA microsatellite markers were analyzed on pedigrees ... more

B.P. Hoh; S.S. Siraj; S.G. Tan; K. Yusoff
genetic breeding; Microsatellites; Musa spp; Primer validation

Thirty-four microsatellite markers (SSRs) were identified in EST and BAC clones from Musa acuminata burmannicoides var. Calcutta 4 and validated in 22 Musa genotypes from the Banana Germplasm Bank of Embrapa-CNPMF, which includes wild and improved diploids. The number of alleles per locus ranged from 2 to 14. The markers were considered highly informative based on their ... more

E.P. Amorim; P.H. Silva; C.F. Ferreira; V.B.O. Amorim; V.J. Santos; A.D. Vilarinhos; C.M.R. Santos; M.T.Souza Júnior; R.N.G. Miller
1711b satellite; Cattle; indicus; Microsatellites; mtDNA; taurus

Our objective was to estimate Bos primigenius taurus introgression in American Zebu cattle. One hundred and four American Zebu (Nellore) cattle were submitted to mtDNA, microsatellite and satellite analysis. Twenty-three alleles were detected in microsatellite analysis, averaging 4.6 ± 1.82/locus. Variance component comparisons of microsatellite allele sizes allowed the ... more

P. Ripamonte; M. Baccaglini; A.S.M. César; A.S.M. César; L.G.G. Figueiredo; J.C.C. Balieiro; A.R. Caetano; F.V. Meirelles
Colombia; Microsatellites; Molecular characterization; Rubus glaucus; SSRs; Thornless blackberry

The species Rubus glaucus, also known as the Andean or “Castilla” blackberry, is one of nine edible species of this genus that grow naturally in Central and South America. In Colombia, this species is the most important of all Rubus species for agricultural and commercial purposes. We used 20 SSRs developed for other Rubus species to characterize 44 Colombian ... more

M. Marulanda; A.M. López; M. Uribe
Medicinal plant; Microsatellites; Population genetics; Tetrastigma hemsleyanum

Tetrastigma hemsleyanum (Vitaceae) is an endangered medicinal plant endemic to China. Because of its widely known efficacy for treating many health problems, wild resources of this species are currently undergoing a rapid decline. Few studies have been conducted examining the population genetics or development of microsatellite loci for this plant. In this study, 14 ... more

Y.H. Wang; N. Chen; Y.C. Zhang; C.X. Fu
Genetic diversity; Microsatellites; Polymorphism; Sogatella furcifera

The whitebacked planthopper Sogatella furcifera (Horváth) (Hemiptera: Delphacidae) is one of the most harmful pests of rice. In this study, 18 polymorphic microsatellite markers were developed from S. furcifera genomic libraries using the fast isolation by amplified fragment length polymorphism of sequences containing repeat protocols. Microsatellite polymorphism was ... more

L. Zhang; G. Li; G.C. He; L.L. Zhu; R. Qin; S.L. Jing
Biofuel; Energy crop; Euphorbiaceae; Genetic diversity; Microsatellites; Physic nut

The last few years have seen a significant increase in the number of large-scale sequencing projects generating whole genome databases. These sequence databases can be surveyed (genome sequence survey) for tandem repeats as an alternative means to develop microsatellites for monitoring and selecting natural populations and cultivars of Jatropha curcas. A total of 100 tandem ... more

R.S. Raposo; I.G.B. Souza; M.E.C. Veloso; A.K. Kobayashi; B.G. Laviola; F.M. Diniz
Animal genetic resources; Genetic management; Microsatellites; mtDNA; Ovis aries

The aim of this study was to analyze genetic diversity and population structure among varieties of White (N = 40), Red (N = 32), and Black (N = 31) Morada Nova hair sheep from flocks in the northeastern Brazilian semiarid region. Fifteen nuclear microsatellite markers and two regions of mitochondrial DNA were used. The intra-population analysis demonstrated that the White variety had ... more

J.S.B. Ferreira; S.R. Paiva; E.C. Silva; C.M. McManus; A.R. Caetano; D.A.E. Façanha; M.A.N. de Sousa
Microsatellites; Polymorphism; Sebastes schlegeli

The Korean rockfish Sebastes schlegeli is a valuable recreational and commercial fish in China, and is cultured in land-based tanks and net cages. Fifteen microsatellite markers were developed for this species, and their polymorphisms were examined in a population. The allele number of the 15 markers ranged from 2 to 13, with an average of 5.933 per locus. The observed and ... more

H.T. Ma; C.F. Jia; J.M. Yang; F. Wang; R. Xue; C.H. Han; H.B. Jiang
Exclusion power; Microsatellites; Parentage analysis; Pig; Single nucleotide polymorphism

Parentage analysis and individual identification are recent, promising methods that have been applied to evolutionary and ecological studies, as well as conservation management. Parental exclusion relying on polymorphic microsatellites has been used worldwide in parentage determination, while the low mutation rate and genotyping error rate of single nucleotide polymorphisms (SNPs) make ... more

G.C. Yu; Q.Z. Tang; K.R. Long; T.D. Che; M.Z. Li; S.R. Shuai