Research Article

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06/03/2008
Acid phosphatase; Acridine orange; Development; Drosophila; Programmed cell death; Salivary gland

Programmed cell death (PCD) in insect metamorphosis assumes a great diversity of morphology and controlling processes that are still not well understood. With the objective of obtaining information about the PCD process, salivary glands of Drosophila arizonae and D. mulleri were studied during larval-pupal development. From the results, it can be concluded that the type of ... more

P. Ianella; M.T.V. Azeredo-Oliveira; M.M. Itoyama
02/13/2014
Chicken; Development; Dosage compensation; Epigenetics; Gonad

The chicken (Gallus gallus) embryo has been used as a classic model system for developmental studies because of its easy accessibility for surgical manipulation during embryonic development. Sex determination in birds is chromosomally based (ZZ for males and ZW for females); however, the basic mechanism of sex determination is still unknown. Here, the dynamics of expression of ... more

L.C. Caetano; F.G.O. Gennaro; K. Coelho; F.M. Araújo; R.A. Vila; A. Araújo; deMelo Bernardo; C.R. Marcondes; S.M.Chuva de S. Lopes; E.S. Ramos
11/11/2013
DNA damage; Genetic analysis; Hair shaft; Mitochondrial DNA; Museum collection; Study skin

The condition of mtDNA in hair shafts preserved in a museum was examined using 30 study skins of masked palm civets, Paguma larvata (Viverridae), collected between 1924 and 2011. Comparisons of extracts from fresh and burnt alum-fixed hair shafts showed that burnt alum, which is commonly used in taxidermy, had no harmful effect on the amount of total DNA and lengths of the mtDNA ... more

N. Kurihara
03/18/2005
Esterases; Hybrid; Peacock bass; Tucunarés

Esterase (Est) and esterase-D (Est-D) electrophoretic patterns identified by starch gel electrophoresis of skeletal muscle protein extracts of 184 specimens of three species of peacock bass, locally known as tucunarés (Cichla monoculus, C. temensis and Cichla sp), plus four specimens of a supposed hybrid (C. monoculus vs C. temensis), collected from the Central ... more

A.Saturnino Teixeira; Sda Silva de Oliveira
10/31/2011
Hybrid; Microsatellite; Olive flounder; Starry flounder

An interspecific artificial hybrid was produced between two economically important aquaculture flatfish: olive flounder (Paralichthys olivaceus) and starry flounder (P. stellatus). This hybrid displays the rapid growth characteristic of the former and tolerance to low temperatures and low salinity of the latter, but the genetics of inheritance in this hybrid have not ... more

J.H. Kang; Y.K. Kim; J.Y. Park; C.M. An; M.M. Nam; S.G. Byun; B.I. Lee; J.H. Lee; T.J. Choi
10/20/2014
DNA methylation; Heterosis; Hybrid; Maize; Methylation-sensitive amplification polymorphism

Heterosis is the superior performance of heterozygous individuals and has been widely exploited in plant breeding, although the underlying regulatory mechanisms still remain largely elusive. To understand the molecular basis of heterosis in maize, in this study, roots and leaves at the seedling stage and embryos and endosperm tissues 15 days after fertilization of 2 elite hybrids and ... more

T.J. Liu; L.F. Sun; X.H. Shan; Y. Wu; S.Z. Su; S.P. Li; H.K. Liu; J.Y. Han; Y.P. Yuan
04/28/2015
Breeding; Chromosome stickiness; Guineagrass; Hybrid; Meiosis; Poaceae

Chromosome stickiness has been studied in several species of higher plants and is characterized by sticky clumps of chromatin resulting in sterility. Chromosome stickiness was recorded in Panicum maximum hybrid plants that were cultivated in the field. In the meiocytes affected, chromosomes clumped into amorphous masses that did not orient themselves on the equatorial plate, and ... more

C. Pessim; M.S. Pagliarini; N. Silva; L. Jank
05/25/2010
Genetic analysis; RAPD; Wild olives

Genetic similarities and distances between wild-type olives in Turkey were studied using an RAPD-PCR assay. Seven wild olive tree samples were collected from villages in Manisa and Izmir provinces. Genomic DNA was extracted from young leaves and the RAPD-PCR assay was used to generate RAPD markers. Sixty-five random primers obtained from Operon Technologies were tested for the assay (OP- ... more

M. Sesli; E.D. Yegenoglu
09/12/2013
Dwarf mutant; Genetic analysis; Gibberellic acid; SSR analysis; Wheat

A spontaneous dwarf mutant of wheat was found in an F5 generation line derived from a cross between Huamai No. 9 and Een No. 1 in 1998; it was named Huaai 01. We characterized the genetic pattern of Huaai 01 and mapped the gene controlling the dwarf trait. This dwarf mutant was found insensitive to exogenous gibberellic acid treatment, based on the length of the first leaf and ... more

X.J. Jin; D.F. Sun; H.Y. Li; Y. Yang; G.L. Sun
04/02/2013
Equilibrium point; Genetic analysis; System dynamics model; Th1-Th2

In the human immune system, T-helper cells are able to differentiate into two lymphocyte subsets: Th1 and Th2. The intracellular signaling pathways of differentiation form a dynamic regulation network by secreting distinctive types of cytokines, while differentiation is regulated by two major gene loci: T-bet and GATA-3. We developed a system dynamics model to simulate the differentiation ... more

Q. Wang; M. Li; L.C. Xia; G. Wen; H. Zu; M. Gao

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