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Amazon basin; Chelonians; Iaçá; Mitochondrial DNA; Podocnemis sextuberculata

The chelonians are, in general, important for the economy of the traditional populations of the Amazon region, especially as a source of animal protein. Furthermore, sub-products, such as eggs and fat, are utilized in the manufacture of cosmetics, and the plastron and carapace are used in the manufacture of adornments. The freshwater turtle species Podocnemis sextuberculata, ... more

T.J. Silva; L.A.S. Monjeló; M.N.S. Viana; J.C. Pezzuti; P.C.M. Andrade; R.C. Vogt; I.P. Farias
Black rockfish; Genetic structure; Hatchery strain; Microsatellite loci; Sebastes inermis; Wild population

The population structure of the black rockfish, Sebastes inermis (Sebastidae), was estimated using 10 microsatellite loci developed for S. schlegeli on samples of 174 individuals collected from three wild and three hatchery populations in Korea. Reduced genetic variation was detected in hatchery strains [overall number of alleles (NA) = 8.07; allelic ... more

H.S. An; E.M. Kim; J.H. Lee; J.K. Noh; C.M. An; S.J. Yoon; K.D. Park; J.I. Myeong
Genetic structure; Korean rockfish; Microsatellite loci; Sebastes schlegeli

The Korean rockfish (Sebastes schlegeli) is an important commercial fish that is widely used in aquaculture. We isolated and characterized 18 polymorphic microsatellite loci from the Korean rockfish using a (GT)13-enriched genomic library. Polymorphism was assessed in 48 individuals from a single population collected from the northern coastal waters of the Yellow Sea. ... more

C.C. Bai; S.F. Liu; Z.M. Zhuang; L. Lin; H. Wang; Y.Q. Chang
Engraulis japonicus; Japanese anchovy; Microsatellite loci; Population structure

We isolated and characterized 14 polymorphic microsatellite loci in the Japanese anchovy (Engraulis japonicus) using a (GT)13-enriched genomic library. The numbers of alleles per locus ranged from 6 to 31, with a mean of 17.8. The observed and expected heterozygosities ranged from 0.180 to 0.949 and from 0.172 to 0.966, with means of 0.731 and 0.825, respectively. All 14 loci ... more

L. Lin; L. Zhu; S.F. Liu; Y.Q. Su; Z.M. Zhuang
Artificial reproduction; Black rockfish; Genetic variability; Microsatellite loci; Mitochondrial DNA; Sebastes inermis

The black rockfish, Sebastes inermis (Sebastidae), is an important commercial fishery resource in Korea. As a preliminary investigation into the effect of artificial reproduction in a hatchery facility, the genetic divergence between parent and offspring populations of black rockfish was accessed using 10 polymorphic nuclear microsatellite DNA loci and a mitochondrial (mt) ... more

H.S. An; J.W. Lee; J.Y. Park; J.I. Myeong; C.M. An
Genetic variability; Korean threadsail filefish; Microsatellite loci; Next-generation sequencing; Stephanolepis cirrhifer

The threadsail filefish, Stephanolepis cirrhifer (Monacanthidae), is found mainly in the western Pacific. It is intensively caught in Korea and is a highly appreciated seafood delicacy. Consequently, the natural population of this species has drastically decreased, despite introductions from hatcheries. To provide information necessary for its conservation and management, we ... more

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Japanese Spanish mackerel; Microsatellite loci; Population structure; Scomberomorus niphonius

We isolated and characterized 21 polymorphic microsatellite loci in Japanese Spanish mackerel (Scomberomorus niphonius) using a (GT)13-enriched genomic library. Forty individuals were collected from Qingdao, China. We found 3 to 24 alleles per locus, with a mean of 8.8. The observed and expected heterozygosities ranged from 0.263 to 0.975 and from 0.385 to 0.946, with ... more

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Atrina pectinata; Microsatellite loci; Polymorphic

In this study, we isolated 21 novel polymorphic microsatellite DNA loci from the pen shell Atrina pectinata using magnetic-bead hybridization enrichment. The characteristics of these loci were tested using a population of 30 individuals collected from the Penglai coast, Shandong Province. The number of alleles ranged from 2 to 13, and polymorphism information content (PIC) varied ... more

B. Wu; L.J. Bai; A.G. Yang; L.Q. Zhou; Z.H. Liu
Cephalopoda; Enriched genomic library; Microsatellite; Sepioteuthis lessoniana

Owing to their codominant, multiallelic, and highly polymorphic nature, microsatellite markers have been used widely in population genetics and biological resource conservation studies. To investigate the genetic structure of Sepioteuthis lessoniana, we developed 24 microsatellite DNA markers and assessed the polymorphism of each locus in a wild S. lessoniana population ... more

X.D. Zheng; Z.R. Zuo; X.J. Su; Q. Li
16S rRNA gene; Amazon basin; Bacterial diversity; Negro River; Solimões River

The microbiota of the Amazon River basin has been little studied. We compared the structure of bacterial communities of the Solimões and Negro Rivers, the main Amazon River tributaries, based on analysis of 16S rRNA gene sequences. Water was sampled with a 3-L Van Dorn collection bottle; samples were collected at nine different points/depths totaling 27 L of water from each river. Total ... more

J.C.C. Peixoto; L. Leomil; J.V. Souza; F.B.S. Peixoto; S. Astolfi-Filho