Research Article

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11/11/2014
Astrocytes; Differentially expressed genes; Functional analysis; Module analysis; Optic nerve

Microarray data of astrocytes extracted from the optic nerves of donors with and without glaucoma were analyzed to screen for differentially expressed genes (DEGs). Functional exploration with bioinformatic tools was then used to understand the roles of the identified DEGs in glaucoma. Microarray data were downloaded from the Gene Expression Omnibus (GEO) database, which contains 13 ... more

Y. Wu; W.D. Zang; W. Jiang
04/30/2014
CHSP; Functional analysis; Ginkgo biloba; Transgenic tobacco

The regulative sequence (2273 bp) of the chalcone synthase gene promoter of biloba was cloned by genomic walking. A 2273-bp promoter 5' upstream translation start site of GbCHS was cloned and designated as GbCHSP. pBI121+CHSP:GUS and pBI121-35S:GUS were constructed and transformed into tobacco by LBA4404. We found that GbCHSP could drive ... more

L.L. Li; H. Cheng; H.H. Yuan; F. Xu; S.Y. Cheng; F.L. Cao
12/08/2009
Genetic diversity; Germplasm conservation; Maize; Random amplified polymorphic DNA

Ten maize accessions (NC-9, A50-2, M-14, B-42, NC-3, T-7, N-48-1, B-34, USSR, and WFTMS) were studied to estimate the genetic distance on molecular level by random amplified polymorphic DNA. These accessions were selected on the basis of their variable responses against different levels of moisture. Twenty-five primers were used to test genetic diversity, of which 14 were observed to be ... more

M. Aslam; F.S. Awan; I.A. Khan; A.I. Khan
03/17/2009
Allele dosage; Endosperm constitution; Maize; Semiquantitative polymerase chain reaction

The lack of informativity of samples from heterozygotic individuals is one of the hindrances in the mapping of quantitative trait loci of outbred populations, since it is not normally possible to identify the origin of each allele. One way to include these individuals in analyses would be to genotype their endosperm, considering that a heterozygote (Aa) has AAa or Aaa ... more

F.A. Martins; P.C.S. Carneiro; C.T. Guimarães; J.V. Magalhães; J.E.S. Carneiro; C.D. Cruz
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Genetic variation; Maize; Quantitative traits; RAPD markers; Zea mays

We compared DNA-based genetic diversity estimates with conventional estimates by investigating agronomically important traits in maize grown in the northwestern region of Pakistan. RAPD markers were used to characterize 10 commonly cultivated maize genotypes. The same material was tested for phenotypic variation of quantitative traits using replicated field trials. The genetic distances ... more

M.M. Shah; S.W. Hassan; K. Maqbool; I. Shahzadi; A. Pervez
06/11/2010
High amylase; Maize; marker-assisted selection; Starch branching enzyme

The ae (amylose extender) recessive mutant alleles in maize are an important genetic resource for the development of high-amylose cultivars. On the basis of ae allele sequences (from the National Center for Biotechnology Information), the ae mutant alleles were cloned from high-amylose maize and the allelic Ae gene from common maize luyuan92 inbred ... more

F. Chen; S.W. Zhu; Y. Xiang; H.Y. Jiang; B.J. Cheng
02/28/2013
Ear leaf; Maize; Nitrogen regime; QTL; Recombinant inbred line

The ear leaf is one of the most important leaves in maize (Zea mays); it affects plant morphology and yield. To better understand its genetic basis, we examined ear leaf length, ear leaf width, and ear leaf area for quantitative trait locus (QTL) mapping in a recombinant inbred line population under two nitrogen regimes. Nine QTLs, on chromosomes 1 (one), 2 (one), 3 (one), 4 ( ... more

Z.P. Zheng; X.H. Liu
06/13/2013
Half-sib; Heritability; Khyber Pakhtunkhwa; Maize; Pahari; Recurrent selection

This research was carried out at the Agriculture Research Farm, Khyber Pakhtunkhwa Agricultural University, Peshawar, and the Cereal Crops Research Institute, Pirsabak, Nowshera, during 2009 and 2010. Half-sib families (HS) derived from the maize variety Pahari in spring crop seasons 2009 and 2010 were developed at the Cereal Crops Research Institute. All HS families were detasseled well ... more

M. Noor; D. Shahwar; H. Rahman; H. Ullah; F. Ali; M. Iqbal; I.A. Shah; I. Ullah
04/17/2013
Maize; Nitrogen regime; Plant architecture; QTL

Maize (Zea mays L.) is one of the most important cereal crops worldwide, and increasing the grain yield and biomass has been among the most important goals of maize production. The plant architecture can determine the grain yield and biomass to some extent; however, the genetic basis of the link between the plant architecture and grain yield/biomass is unclear. In this study, an ... more

Z.P. Zheng; X.H. Liu
06/25/2012
Expression; FKBPs; Gene duplication; Maize

The receptors for the immunosuppression drugs FK506 and rapamycin are called FKBPs (FK506-binding proteins). FKBPs comprise a large family; they are found in many species, including bacteria, fungi, animals, and plants. As a class of peptidyl-prolyl cis-trans isomerase enzymes, the FKBP genes have been the focus of recent studies on plant stress tolerance and immunology. We ... more

W.W. Wang; Q. Ma; Y. Xiang; S.W. Zhu; B.J. Cheng

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