Research Article

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10/05/2015
Hepatocellular carcinoma; Phosphorylation; Site-directed mutagenesis; Stathmin

We constructed hepatocellular carcinoma (HCC) cells that stably express stathmin with a Ser25 phosphorylation site mutation (stathmin S25A). We used the polymerase chain reaction for site-directed mutagenesis, constructed a stathmin S25A plasmid, and verified the results by restriction enzyme cleavage and sequencing technology. Using the liposome transfection method, stathmin wild-type ... more

J. Du; Z.H. Tao; J. Li; Y.K. Liu; L. Gan

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