Research Article

Related GMR Articles

09/12/2011
Genetic marker; Larus saundersi; Microsatellite; Polymorphism; Saunders’s gull

Nine polymorphic microsatellite loci were isolated, using tetranucleotide repeat oligonucleotide probes from an enriched DNA library of the globally “vulnerable” Saunders’s gull (Larus saundersi), collected from the Yancheng coastal wetland, one of the three remaining breeding sites in China. Six breast muscle tissues and 16 blood samples from 22 gulls and eight eggshell membrane ... more

H.X. Jiang; S.L. Ren; Y.Q. Hou; M. Yang; X.B. Wu
08/16/2011
Invasive species; Microsatellite; Polymorphism; Pseudorasbora parva

The Asiatic topmouth gudgeon, Pseudorasbora parva, is recognized as one of the most invasive fish species in many countries outside of Asia. We isolated and characterized 19 microsatellite loci from P. parva. The polymorphism of these 19 loci was tested on 40 individuals of P. parva sampled from a wild population located in Ezhou, Hubei province of China. The ... more

C. Zeng; Y. Gul; K. Yang; L. Cui; W.M. Wang; Z.X. Gao
04/26/2011
Duchenne muscular dystrophy; Dystrophin gene; Han population genetics; Microsatellite; Polymorphism

To look for novel microsatellites in the dystrophin gene for the diagnosis of Duchenne muscular dystrophy, candidate microsatellite sites in the dystrophin gene were analyzed with the SSRHunter software and were also genotyped. Among the 15 candidate microsatellite sites, three novel microsatellite sites in the 60th, 30th, and 2nd intron were found to have a high degree of polymorphism. ... more

R.F. Sun; Y.S. Zhu; J.L. Feng; Z. Tian; W.J. Kuang; Y. Liu; H.B. Zhang; S.B. Li
02/15/2011
Genetic marker; Microsatellite; Pelophylax hubeiensis; Polymorphism

Twelve polymorphic microsatellite loci were isolated from an (AC)n- and (AG)n-enriched DNA library for the endemic Chinese frog Pelophylax hubeiensis (Ranidae). The number of alleles per locus ranged from two to eight, with a mean of 5.17. The observed and expected heterozygosities ranged from 0.226 to 0.839 and from 0.204 to 0.826, with means of 0.568 and 0. ... more

J.C. Yan; F.H. Zhu; H.L. Wu
12/10/2013
Microsatellite; Oplegnathus fasciatus; Polymorphism

The rock bream fish Oplegnathus fasciatus is one of the most popular aquaculture species in China. In the present study, 15 novel polymorphic microsatellite loci were isolated and characterized from a wild population of O. fasciatus from the Zhoushan coast of China. The number of alleles per polymorphic locus ranged from 4 to 9 in a sample of 30 individuals. Observed and ... more

X.X. Sun; D.D. Xu; B. Lou; S.L. Li; W. Zhan; Y.R. Zhang; J. Xin
12/04/2013
Microsatellite; Nibea albiflora; Polymorphism

Eleven novel microsatellite loci were isolated from a (CA)10-enriched genomic DNA library of Nibea albiflora. The characteristics of these microsatellites were determined in a sample of 48 N. albiflora individuals. The number of alleles at the 11 microsatellite loci ranged from 5 to 25, with an average of 13.5 per locus. The observed and expected ... more

D.D. Xu; B. Lou; S.L. Li; Y.R. Zhang; J. Xin; W. Zhan
11/22/2013
Microsatellite; Molecular markers; Polymorphism; Portunus trituberculatus

The Swimming crab Portunus trituberculatus (Portunidae) is an important economically food species. To provide molecular markers for P. trituberculatus, we isolated and characterized polymorphic microsatellite markers. We developed a 5'-anchored genomic library of P. trituberculatus DNA, and derived 45 positive clones. We designed 30 pairs of primers from the ... more

L.P. Ren; Y. Qin; X.C. Li; Y.N. Sun; R.X. Wang
05/14/2012
Microsatellite; Polymorphism; Taxus chinensis var. mairei

We isolated and characterized 11 microsatellite loci for the Chinese yew, Taxus chinensis var. mairei, an endangered tree species in China, by constructing a (CA)12-enriched library. The number of alleles per locus ranged from 5 to 10. The observed heterozygosities ranged from 0.2500 to 0.8333 and the expected heterozygosities ranged from 0.5196 to 0.8680. No ... more

H. Xue; C.H. Lu; X.B. Wu
02/16/2012
Cyclina sinensis; Genetic diversity; Microsatellite; Polymorphism

The Venus clam, Cyclina sinensis, is one of the most important bivalves in China marine aquaculture. Using (CA)15-enriched genomic libraries of this species, nine novel polymorphic microsatellite loci were isolated and characterized. The mean number of observed alleles per locus was 16 (range 8-24). The observed and expected heterozygosity ranged from 0.119 to 0.872 ... more

J.X. Wang; T.J. Xu; L. Wei; F.X. Meng; R.X. Wang
03/17/2014
Chinese muntjac; Microsatellite; Muntiacus reevesi; Polymorphism; Population genetics

Eight polymorphic microsatellite markers for Muntiacus reevesi were identified and characterized in this study. The number of alleles per locus ranged from 3 to 10 across 24-48 samples. The loci showed expected and observed heterozygosities of 0.577-0.876 and 0.387-0.933, respectively, with an average polymorphic information content value of 0.682. These markers should be a ... more

H. Wang; X. Luo; W.B. Shi; B.W. Zhang

Pages