Research Article

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Differentially expressed gene; Disc degeneration; Dysfunctional pathway

Disc degeneration is strongly associated with back or neck pain, sciatica, and disc herniation or prolapse. It places an enormous economic burden on society and can greatly affect quality of life. Alternative treatment approaches, such as genetic therapies, are urgently needed to slow or reverse the disc degeneration process. We downloaded gene expression data from Gene Expression Omnibus ... more

Y. Chen; K. Chen; M. Li; C. Li; H. Ma; Y.S. Bai; X.D. Zhu; Q. Fu
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Differentially expressed gene; Osteoarthritis; Protein-protein interaction network

The purpose of this study was to identify genes and pathways for osteoarthritis (OA) diagnosis and therapy. We downloaded the gene expression profile of OA from Gene Expression Omnibus (GEO) database including 10 early OA, 9 late OA, and 5 normal control samples. Next, we screened differentially expressed genes (DEGs) between early- and late-stage OA samples comparing with healthy control ... more

Y.C. Zhu; B.Y. Deng; L.G. Zhang; P. Xu; X.P. Du; Q.G. Zhang; B. Yang
01/08/2014
Differentially expressed gene; Function enrichment analysis; Microarray; Preadipocytes

We looked for differentially expressed genes at different stages of preadipocyte differentiation and examined their functions, based on DNA microarrays of preadipocytes obtained from healthy subjects undergoing cosmetic liposuction. We downloaded gene expression profile GSE25910 from the Gene Expression Omnibus database and identified the differentially expressed genes with packages in R ... more

J.Y. Du; H. Yang; D.R. Tian; Q.M. Wang; L. He
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Differentially expressed gene; Hub protein; Renal dysfunction; Renal medullary hypertension

The aim of this study was to identify differentially expressed genes (DEGs) in renal medullary hypertension and reveal their pathogenic mechanisms. We downloaded the gene expression profile of GSE28360 from the Gene Expression Omnibus database. The profile included 14 samples (5 normal and 9 hypertension). The DEGs in normal and disease samples were distinguished with a false-discovery ... more

Y.B. Wu; W.D. Zang; W.Z. Yao; Y. Luo; B. Hu; L. Wang; Y.L. Liang
05/23/2014
Asterias rubens; Gene expression; High-throughput sequencing; Real-time qPCR; Reference gene; UBA52

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A.F. Sadritdinova; A.A. Dmitriev; A.V. Snezhkina; M.S. Belenikin; G.S. Krasnov; O.G. Manylov; A.A. Kudryavtsev; N.V. Melnikova; A.S. Speranskaya; M.V. Darii; V.A. Lakunina; L.A. Uroshlev; A.O. Smurov; O.A. Stepanov; A.V. Kudryavtseva
11/17/2009
Genetic diversity; Polymerase chain reaction; Random amplified polymorphic DNA; Sugarcane

Genetic diversity of 20 sugarcane accessions in Pakistan was studied using 21 random amplified polymorphic DNA markers. The mean genetic distance between the cultivars was 39.03%, demonstrating that a large part of the genome is similar among the accessions. This probably arises from a lack of parental diversity, with few clones, which are themselves related, contributing to the parentage ... more

F.A. Khan; A. Khan; F.M. Azhar; S. Rauf
08/26/2011
RAPD; Red rot; SSR; Sugarcane; Variability

Sugarcane breeding under climatic conditions of Pakistan is very difficult due to unavailability of viable fuzz (seed). Somaclonal variation can provide an alternative for improvement of existing genotypes. Six hundred and twenty-seven somaclones were developed from sugarcane genotype S97US297, and protocols for callogenesis and organogenesis were developed using Murashige and Skoog ... more

M.T.H. Shahid; F.A. Khan; A. Saeed; I. Fareed
08/24/2010
DNA marker; Fingerprinting; Genetic distance; RAPD; Sugarcane

Forty sugarcane genotypes (clones), including elite lines, commercial cultivars of Saccharum officinarum and S. barberi clones, were fingerprinted with 30 RAPD markers, using a PCR-based marker assay. The genetic distance for RAPD data was determined according to Nei, and relationships between accessions were graphed in a dendrogram. Genetic distance values ranging from ... more

S. Nawaz; F.A. Khan; S. Tabasum; M. Zakria; A. Saeed; M.Z. Iqbal
03/16/2010
DNA marker; Fingerprinting; Genetic distance; RAPD; Sugarcane

DNA profiles of 40 sugarcane genotypes were constructed with 30 RAPD markers. Sugarcane genotypes of both Saccharum officinarum and S. barberi were included in this study. Multiple alleles were detected from each RAPD; there was a high level of polymorphism. On average, 7.93 alleles were produced per primer, giving a total of 238 alleles. The genetic distances between ... more

S. Tabasum; F.A. Khan; S. Nawaz; M.Z. Iqbal; A. Saeed
10/15/2013
16S rRNA; Endophytic bacteria; Microbial diversity; Sugarcane

Sugarcane is an economically important culture in Brazil. Endophytic bacteria live inside plants, and can provide many benefits to the plant host. We analyzed the bacterial diversity of sugarcane cultivar RB-72454 by cultivation-independent techniques. Total DNA from sugarcane stems from a commercial plantation located in Paraná State was extracted. Partial 16S rRNA genes were amplified ... more

G.S. Magnani; L.M. Cruz; H. Weber; J.C. Bespalhok; E. Daros; V. Baura; M.G. Yates; R.A. Monteiro; H. Faoro; F.O. Pedrosa; E.M. Souza

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