Brief Note

Related GMR Articles

Bodhesin; Capra hircus; cDNA; Prokaryotic expression; Recombinant protein; Spermadhesin

Low purification efficiency and incomplete characteriza­tion of male goat (buck) spermadhesins (Bdhs) prompted us to develop an effective system to produce recombinant Bdhs (rBdhs). Bdh-2 cDNA was inserted into a prokaryotic expression plasmid, pTrcHis TOPO. The pTrcHis-Bdh-2 system was constructed to produce a His6 fusion protein in Escherichia coli Top10 cells. Recombinant ... more

J.B. Cajazeiras; L.M. Melo; E.S. Albuquerque; G. Rádis-Baptista; B.S. Cavada; V.J.F. Freitas
Ag-NORs; Capra hircus; Chromosomal banding; Karyotype

In order to provide the first cytogenetic data of naturalized and threatened goat breeds from northeastern Brazil, cytogenetic analyses were carried out in individuals of Repartida and Moxotó breeds raised in Bahia and Ceará States. Males and females of both breeds had 2n = 60, with 29 autosomal acrocentric pairs plus the sex chromosome pair. The number of nucleolar organizer region (NOR ... more

R.P. Santos; P.R.A.M. Affonso; D. Diniz; A.S. Medrado; K.M. Silva; P.L.S. Carneiro
Capra hircus; Cytokine; Gastrointestinal infection; Single nucleotide polymorphism

Cytokines are small cell-signaling proteins that play an important role in the immune system, participating in intracellular communication. Four candidate genes of the cytokine family (IL2, IL4, IL13, and IFNG) were selected to identify Single Nucleotide Polymorphisms (SNPs) that might be associated with resistance to gastrointestinal endoparasites in ... more

F.A. Bressani; P.C. Tizioto; R. Giglioti; S.L.C. Meirelles; R. Coutinho; C.L. Benvenuti; W. Malagó-Jr.; M.A. Mudadu; L.S. Vieira; L.G. Zaros; E. Carrilho; L.C.A. Regitano
Major histocompatibility complex; Poultry; Single nucleotide polymorphisms; TAP1; TAP2

TAP1 and TAP2 genes code for the two subunits of the transporter associated with antigen processing (TAP), and in chicken they are located between the two MHC class I genes. Using primers based on chicken sequences, the genomic regions corresponding to chicken TAP1 exons 6 to 7 and TAP2 exons 4 to 6 (which encode portions of the chicken TAP1 and ... more

L. Sironi; B. Lazzari; P. Ramelli; A. Stella; P. Mariani
Allelic polymorphism; Balancing selection; Cynoglossus semilaevis; Major histocompatibility complex

Major histocompatibility complex (MHC) genes play an important role in the immune response of vertebrates. Allelic polymorphism and evolutionary mechanism of MHC genes have been investigated in many mammals, but much less is known in teleosts. We examined the polymorphism, gene duplication and balancing selection of the MHC class II DAB gene of the half-smooth tongue sole ( ... more

T.J. Xu; Y.N. Sun; R.X. Wang
Cloning; Expression; Major histocompatibility complex; Stone flounder

Major histocompatibility complex (MHC) class II genes play important recognition roles in the immune system in vertebrates. We cloned the MHC class II genes A and B in the stone flounder (Kareius bicoloratus). The full-length cDNA and DNA sequences of both genes were obtained, and their characteristic motifs were analyzed. The DNA sequence of stone flounder MHC ... more

J. Jiang; C. Li; Q. Zhang; X. Wang
Bubalus bubalis; Major histocompatibility complex; PCR-RFLP; Polymorphism

The DRB3 gene is an MHC class II gene that has a high degree of polymorphism with more than 100 alleles described in cattle. This variation contributes to differences among individuals in immune responsiveness and disease resistance. In this study, we searched for allelic variants in exon 2 of the DRB3 gene in 80 river buffaloes of three breeds in Brazil using a PCR-RFLP ... more

N.B. Stafuzza; L.M. Olivatto; B.C.M. Naressi; H. Tonhati; M.E.J. Amaral-Trusty; N.B. Stafuzza; L.M. Olivatto; B.C.M. Naressi; H. Tonhati; M.E.J. Amaral-Trusty; N.B. Stafuzza; L.M. Olivatto; B.C.M. Naressi; H. Tonhati; M.E.J. Amaral-Trusty