Research Article

Related GMR Articles

Glutamine synthetase; Immune response; Messenger RNA expression; Tegillarca granosa; Vibrio parahaemolyticus

The blood cockle, Tegillarca granosa, is a widely consumed clam in the Indo-Pacific region. Glutamine synthetase (GS) is an enzyme that plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine. We identified the GS of T. granosa (Tg-GS) from hemocytes by 3'- and 5'-rapid amplification of cDNA ends ( ... more

Y.B. Bao; L. Li; M.X. Ye; Y.H. Dong; W.X. Jin; Z.H. Lin
Cytokine; Immune response; Porcine circovirus type 2; Pseudorabies attenuated vaccine

We investigated the immune response effects of porcine circovirus type 2 (PCV2) on cells inoculated with pseudorabies attenuated vaccine (PRV). Real-time PCR was used to detect the mRNA expression levels of the regulatory cytokines IL-4, IL-10, IL-12p40, and IFN-γ in pig peripheral blood mononuclear cells, after in vitro single vaccination and co-inoculation with PCV2 and the PRV ... more

F. Gao; J.L. Xie; C.W. Jia; H.Y. Ren; S.H. Zhou
Big defensin; Hyriopsis cumingii; Immune response; Induced expression

Antimicrobial peptides (AMPs), of which big defensins are examples, are an important component of the natural defenses of most living organisms, and possess remarkable microbicidal activities. In the present study, using expressed-sequence tag sequences from a cDNA library and RACE, the full-length cDNA sequence of the big defensin gene from the triangle-shell pearl mussel, Hyriopsis ... more

G.L. Wang; X.L. Xia; X.L. Li; S.J. Dong; J.L. Li
Cisplatin; Immune response; Lactobacillus; Lung cancer

The intestinal microflora affects inflammation and immunity, not only locally at the mucosal level but also systemically, raising the question of whether the microflora affects inflammatory processes that contribute to cancer and its therapy. Prebiotics have also been found to play an antitumor role that is not limited to the gut. We investigated the antitumor roles of the intestinal ... more

Q.F. Gui; H.F. Lu; C.X. Zhang; Z.R. Xu; Y.H. Yang
Ammonia concentration; broiler; Immune response; Relative humidity

To investigate the effect of ammonia (NH3) and humidity on the immune response of broilers, broilers were exposed to 30 or 70 mg/kg atmospheric NH3 for 21 days. Additionally, birds were exposed to 35, 60, and 85% relative humidity (RH). The relative weights of lymphoid organs, serum total protein, serum globulin, serum albumin, serum lysozyme, proliferation index of ... more

F.X. Wei; X.F. Hu; B. Xu; M.H. Zhang; S.Y. Li; Q.Y. Sun; P. Lin
Immune response; Pearl oyster; Pinctada martensii; Purple acid phosphatase

Purple acid phosphatases (PAPs), also known as type 5 acid phosphatases, are widely present in animals, plants, and fungi. In mammal, PAP was reported to participate in immune defense and bone resorption. In this study, the characteristics and potential functions of a PAP gene from pearl oyster Pinctada martensii (pm-PAP) were examined. The Pm-PAP cDNA was found to be 2777 base ... more

Q.H. Wang; Y. Jiao; X.D. Du; X.X. Zhao; R.L. Huang; Y.W. Deng; F. Yan
Differentially expressed genes; Regulation network; Testicular seminoma

Testicular seminoma has become the most common solid malignancy in young men, especially in the 20s group. We obtained the gene expression profile of human testicular seminoma cells from NCBI, identified the differentially expressed genes of testicular seminoma cells of different stages, and constructed the regulation networks of different stages of testicular seminoma using ... more

J.J. Sha; Y.H. Dong; D.M. Liu; J.J. Bo; Y.R. Huang; Z. Li; P. Ping
CMAP; Differentially expressed genes; Pulmonary hypoxia-related diseases; Similar drugs

Exposure of humans to low levels of environmental oxygen results in alveolar hypoxia and normally causes chronic pulmonary hypertension and morphological alterations of precapillary pulmonary vessels. In this study, the microarray dataset GSE11341 was used to identify potential differentially expressed genes related with human lung microvascular endothelial cell hypoxia. In addition, gene ... more

L. Zhou; L.M. Wang; H.M. Song; Y.Q. Shen; W.J. Xu; J.H. Xu; Y. Liu; W.W. Yan; J.F. Jiang
Differentially expressed genes; Epstein-Barr virus-positive nasopharyngeal carcinoma; Target genes; Typical miRNAs

This study aims to identify the crucial miRNAs in Epstein-Barr virus-positive nasopharyngeal carcinoma (NPC) and their target genes. Gene expression profile data (GSE12452) that included 31 NPC and 10 normal nasopharyngeal tissue specimens were downloaded. Differentially expressed genes (DEGs) were identified using significance analysis of microarrays. The underlying function of DEGs was ... more

L.H. Zhu; X.T. Miao; N.Y. Wang
Endothelial progenitor cells; High blood pressure; Prostacyclin; Pulmonary hypertension

The aim of this study was to investigate the correlation between circulating endothelial progenitor cell (EPC) levels and plasma prostacyclin (PGI2) concentrations in pulmonary hypertension (PH) rat models. Monocrotaline was used to induce PH, and the number of CD34/Flk-1-double-positive mononuclear cells in peripheral CD45-negative blood was counted by flow cytometry, which reflected ... more

Y. Song