Research Article

Related GMR Articles

09/30/2004
Bioinformatics; Biotechnology; Brazil; Genomics; Microbial genome project; Prokaryotes

Since the Haemophilus influenzae genome sequence was completed in 1995, 172 other prokaryotic genomes have been completely sequenced, while 508 projects are underway. Besides pathogens, organisms important in several other fields, such as biotechnology and bioremediation, have also been sequenced. Institutions choose the organisms they wish to sequence according to the ... more

P.Borges San Celestino; L.Rodrigues de Carvalho; L.Martins de Freitas; N.Florêncio Martins; L.Gustavo Ca Pacheco; A. Miyoshi; V. Azevedo; F.Alves Dorella
09/30/2004
Association mapping; Eucalyptus; Genolyptus; Genomics; QTL

The advent of high throughput genomic technologies has opened new perspectives in the speed, scale and detail with which one can investigate genes, genomes and complex traits in Eucalyptus species. A genomic approach to a more detailed understanding of important metabolic and physiological processes, which affect tree growth and stress resistance, and the identification of ... more

D. Grattapaglia
05/21/2012
Chicken; EST; Gallus gallus; Genomics; Microsatellites; SSR

Many years of domestication and breeding have given rise to the wide range of chicken breeds that exist today; however, an increasing number of local chicken breeds are under threat of extinction. A comprehensive characterization of chicken markers (especially type I markers) is needed to monitor and conserve genetic diversity in this species. The explosion of genomics and functional ... more

M.R. Bakhtiarizadeh; B. Arefnejad; E. Ebrahimie; M. Ebrahimi
12/30/2004
Bioinformatics; Genetic determinism; Genomics; Human genome; Public understand of science; Science and technology studies

The literature about genomics and bioinformatics achievements in high-impact journals such as Nature and Science has raised disproportionate expectations amongst the general public about fast and revolutionary drugs and breakthroughs in biomedicine. However, the yield obtained by database mining activities has been modest, as reported in the February 2001 issues of ... more

M. Leite
10/04/2007
Data integration; Databases; MySQL; Proteomics; Schistosoma; Transcriptomics

Proteomics correspond to the identification and quantitative analysis of proteins expressed in different conditions or life stages of a cell or organism. Methods used in proteomics analysis include mainly chromatography, two-dimensional electrophoresis and mass spectrometry. Data generated in proteomics analysis vary significantly, and to identify a protein it is often necessary to ... more

A.C. Faria-Campos; R.R. Gomes; F.S. Moratelli; H. Rausch-Fernandes; G.R. Franco; S.V.A. Campos
08/04/2009
Amniotic fluid; Human phosphate binding protein; Proteomics

Human amniotic fluid is of both maternal and fetal origin; it protects the fetus and provides the environment for growth and development of the fetus. We used a proteomics-based approach for targeting and purifying human phosphate binding protein, a member of the DING family of proteins from amniotic fluid, using Blue Sepharose CL-6B, DEAE-Sephacel and gel filtration chroma­tography. The ... more

M. Alam; M. Mahajan; M. Raziuddin; T.P. Singh; S. Yadav
10/03/2011
Biomarkers; Non-tumoral breast tissue; Proteomics

Breast cancer is a complex and heterogeneous disease. In spite of the advances made in recent decades, a better understanding of the intrinsic mechanisms of this disease is crucial. The development of new biomarkers is absolutely necessary to improve diagnosis and prognosis. Research using the proteomic approach has generated interesting results; however, the complexity of the mammary ... more

G.G. Costa; R. Kaviski; L.E.R. Souza; C.A. Urban; R.S. Lima; I.J. Cavalli; E.M.S.F. Ribeiro
12/19/2013
MALDI-TOF MS; Musa acuminate Colla; Proteomics; Two-dimensional gel electrophoresis

To establish a proteomic reference map of Musa acuminate Colla (banana) leaf, we separated and identified leaf proteins using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry (MS). Tryptic digests of 44 spots were subjected to peptide mass fingerprinting (PMF) by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS. Three ... more

Y. Lu; Y.X. Qi; H. Zhang; H.Q. Zhang; J.J. Pu; Y.X. Xie
02/14/2014
Multiple sclerosis; Neuromyelitis optica; Proteomics

This study aimed to identify the proteins that are differentially expressed in sera of multiple sclerosis (MS) patients, neuromyelitis optica (NMO) patients, and normal controls by using a two-dimensional gel electrophoresis (2-DE) assay. Serum samples were collected from the 3 groups, and total proteins were isolated and quantified by using the Bradford assay. The 2-DE and silver ... more

S.F. Jiang; Q.Y. Lu; S. Hu; Y. Chen; X.L. Liu; Y. Yang; M.P. Ding
10/20/2014
Chronic intermittent hypoxia; Gender difference; Isobaric tag for relative and absolute quantitation; Obstructive sleep apnea; Proteomics; Vascular injury

Obstructive sleep apnea (OSA) is an independent risk factor for cardiovascular diseases such as systemic arterial hypertension, ischemic heart disease, stroke, heart failure, atrial fibrillation, and cardiac sudden death. The pathogenesis of cardiovascular disease in OSA is thought to be induced primarily by chronic intermittent hypoxia (CIH), a specific pattern of change in oxygenation ... more

Q.Y. Li; Y. Feng; Y.N. Lin; M. Li; Q. Guo; S.Y. Gu; J.L. Liu; R.F. Zhang; H.Y. Wan

Pages