Research Article

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Tumor necrosis factor receptor-associated factor 3 (TRAF3) is a crucial regulator that suppresses c-Jun N-terminal kinase and non-canonical nuclear factor-kB signaling, but facilitates type I interferon production. To determine TRAF3 function in innate immune responses among birds, particularly chicken, we cloned and characterized the chicken TRAF3 gene (chTRAF3) and detected ... more

H.L. Yang; Z.Q. Feng; S.Q. Zeng; S.M. Li; Q. Zhu; Y.P. Liu
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Bioinformatic analysis; Cloning; GnRH; Jinghai yellow chicken; Prokaryotic expression

The gonadotropin-releasing hormone (GnRH) plays an important role in the control of reproductive functions. Recent studies have reported the occurrence of GnRH molecular variants in numerous species. In this study, the GnRH1 gene from Jinghai yellow chicken was cloned by reverse transcriptase-polymerase chain reaction and transformed into BL21 (DE3) competent cells. The ... more

T. Zhang; G.X. Zhang; K.P. Han; Y. Tang; J.Y. Wang; Q.C. Fan; X.S. Chen; Y. Wei; Y.J. Wang
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C-type lectin fold; Cloning; Metamorphosis; Mizuhopecten yessoensis; mRNA expression

C-type lectins are a superfamily of Ca2+-dependent carbohydrate-recognition proteins that are well known for their participation in pathogen recognition and clearance. In this study, a putative C-type lectin fold (MyCLF) gene was identified from the Japanese scallop Mizuhopecten yessoensis. The full-length of MyCLF was 645 bp, encoding a polypeptide of 167 amino acids ... more

X.B. Bao; C.B. He; C.D. Fu; B. Wang; X.M. Zhao; X.G. Gao; W.D. Liu
01/23/2015
Capsicum annuum L.; CaRCCR gene; Cloning; Expression analysis; Stress response

Opening the porphyrin macrocycle of pheophorbide a and forming the primary fluorescent chlorophyll catabolites are key steps in the chlorophyll catabolism pathway. These steps are catalyzed by pheophorbide a oxygenase and red chlorophyll catabolite reductase (RCCR). In this study, a novel RCCR gene, CaRCCR, was isolated from the pepper (Capsicum annuum ... more

H.J. Xiao; J.H. Jin; W.G. Chai; Z.H. Gong
06/14/2013
Fusion protein; Giant panda; Interleukin-6/interleukin-2; Prokaryotic expression

To construct a fusion cytokine protein with more and stronger bioactivities to enhance the immunity of the cytokine alone, we expressed interleukin (IL)-6/(IL)-2 from giant panda (Ailuropoda melanoleuca) in Escherichia coli as a 59.4-kDa fusion protein. Subsequently, the inclusion bodies were solubilized with 8 M urea and applied onto a Ni-nitrilotriacetic acid column. ... more

Y. Yi; Y.Y. Nian; H.W. Ji; H. Zhang; L. Zhu; Z.W. Xu
11/19/2014
Bioactivity analysis; Giant panda; Interleukin 18; Molecular cloning

Interleukin 18 (IL-18), as a member of IL-1 superfamily, is an important pleiotropic cytokine that modulates Th1 immune responses. In this report, we cloned and identified a homolog of IL-18 in giant panda (Ailuropoda melanoleuca) (designated as AmIL-18) from peripheral blood mononuclear cells stimulated with lipopolysaccharide. The open readin g frame of AmIL-18 cDNA is ... more

Y. Yan; Q. Wang; L.L. Niu; J.B. Deng; J.Q. Yu; Y.Z. Zhang; J.X. Wang; M.M. Yin; X.M. Tan
04/08/2008
Antigenic characterization; Cloning; Expression; GRA5; GRA7; ROP2; Toxoplasma gondii

Toxoplasma gondii is an intracellular obligate protozoan,which infects humans and warm-blooded animals. The aim of the present study was to clone the rop2, gra5 and gra7 genes from T. gondii RH strain and to produce recombinant proteins. The rop2, gra5 and gra7 gene fragments produced by polymerase chain reaction were ... more

M. Igarash; F. Kano; K. Tamekuni; P.M. Kawasaki; I.T. Navarro; O. Vidotto; M.C. Vidotto; R.Z. Machado; J.L. Garcia
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Alternative splicing; Cancer-testis gene; Cloning; ODF3; ODF4

The outer dense fiber (ODF) genes encode proteins that co-assemble along the axoneme of the sperm tail. Recently, it was demonstrated that some ODF genes are aberrantly expressed in tumors, including prostate adenocarcinoma, basal cell carcinoma, and chronic myeloid lymphoma. We cloned ODF3 and ODF4 cDNA from the testis of a patient suffering from ... more

S. Ghafouri-Fard; S. Ghafouri-Fard; M.H. Modarressi
08/29/2012
BAC library; Bubalus bubalis; Cloning; Pulse-field gel electrophoresis

River buffalo genome analyses have advanced significantly in the last decade, and the genome sequence of Bubalus bubalis will be available shortly. Nonetheless, large-insert DNA library resources such as bacterial artificial chromosomes (BAC) are still required for validation and accurate assembly of the genome sequence. We constructed a river buffalo BAC library containing 52, ... more

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12/18/2014
Bioinformatic analysis; Calcium-dependent protein kinases; Cloning; TgCDPK6; Toxoplasma gondii

Toxoplasma gondii is recognized as an opportunistic human pathogen with a worldwide distribution. Development of effective vaccines is considered the only ideal way to control T. gondii infection. However, only one live vaccine is commercially available for use in sheep and goats. Therefore, the identification of more effective antigenic proteins is very ... more

N.Z. Zhang; S.Y. Huang; D.H. Zhou; Y. Xu; J.J. He; X.Q. Zhu

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