Research Article

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Genetically modified organism; PCR; Strip test

Brazilian legislation establishes a labeling limit for products that contain more than 1% material from genetically modified organisms (GMOs). We assessed the sensitivity of the lateral flow strip test in detection of the GMO corn varieties Bt11 and MON810 and the specificity and sensitivity of PCR techniques for their detection. For the strip test, the GMO seeds were mixed with ... more

V.E. Nascimento; E.V.R. Von Pinho; R.G. Von Pinho; A.Ddo Nascime Júnior
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Ten maize accessions (NC-9, A50-2, M-14, B-42, NC-3, T-7, N-48-1, B-34, USSR, and WFTMS) were studied to estimate the genetic distance on molecular level by random amplified polymorphic DNA. These accessions were selected on the basis of their variable responses against different levels of moisture. Twenty-five primers were used to test genetic diversity, of which 14 were observed to be ... more

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Genetic variation; Maize; Quantitative traits; RAPD markers; Zea mays

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M.M. Shah; S.W. Hassan; K. Maqbool; I. Shahzadi; A. Pervez
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High amylase; Maize; marker-assisted selection; Starch branching enzyme

The ae (amylose extender) recessive mutant alleles in maize are an important genetic resource for the development of high-amylose cultivars. On the basis of ae allele sequences (from the National Center for Biotechnology Information), the ae mutant alleles were cloned from high-amylose maize and the allelic Ae gene from common maize luyuan92 inbred ... more

F. Chen; S.W. Zhu; Y. Xiang; H.Y. Jiang; B.J. Cheng
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Ear leaf; Maize; Nitrogen regime; QTL; Recombinant inbred line

The ear leaf is one of the most important leaves in maize (Zea mays); it affects plant morphology and yield. To better understand its genetic basis, we examined ear leaf length, ear leaf width, and ear leaf area for quantitative trait locus (QTL) mapping in a recombinant inbred line population under two nitrogen regimes. Nine QTLs, on chromosomes 1 (one), 2 (one), 3 (one), 4 ( ... more

Z.P. Zheng; X.H. Liu
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Half-sib; Heritability; Khyber Pakhtunkhwa; Maize; Pahari; Recurrent selection

This research was carried out at the Agriculture Research Farm, Khyber Pakhtunkhwa Agricultural University, Peshawar, and the Cereal Crops Research Institute, Pirsabak, Nowshera, during 2009 and 2010. Half-sib families (HS) derived from the maize variety Pahari in spring crop seasons 2009 and 2010 were developed at the Cereal Crops Research Institute. All HS families were detasseled well ... more

M. Noor; D. Shahwar; H. Rahman; H. Ullah; F. Ali; M. Iqbal; I.A. Shah; I. Ullah
04/17/2013
Maize; Nitrogen regime; Plant architecture; QTL

Maize (Zea mays L.) is one of the most important cereal crops worldwide, and increasing the grain yield and biomass has been among the most important goals of maize production. The plant architecture can determine the grain yield and biomass to some extent; however, the genetic basis of the link between the plant architecture and grain yield/biomass is unclear. In this study, an ... more

Z.P. Zheng; X.H. Liu
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DNA methylation; Heterosis; Hybrid; Maize; Methylation-sensitive amplification polymorphism

Heterosis is the superior performance of heterozygous individuals and has been widely exploited in plant breeding, although the underlying regulatory mechanisms still remain largely elusive. To understand the molecular basis of heterosis in maize, in this study, roots and leaves at the seedling stage and embryos and endosperm tissues 15 days after fertilization of 2 elite hybrids and ... more

T.J. Liu; L.F. Sun; X.H. Shan; Y. Wu; S.Z. Su; S.P. Li; H.K. Liu; J.Y. Han; Y.P. Yuan
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Comparative mapping; Genetic map; Integrated linkage map; Maize; Meta-analysis; QTL for disease resistance

We collected data regarding 340 disease resistance quantitative trait loci (QTLs) from the maize genomic database (MaizeGDB). We constructed an integrated linkage map and analyzed this map by using the BioMercator 2.1 software with IBM2 2008 Neighbors genetic linkage map as a reference. We used a meta-analysis method to identify five “consensus” synthetic resistance QTLs located on maize ... more

L. Zhao; H.J. Liu; C.X. Zhang; Q.Y. Wang; X.H. Li
12/14/2006
Crinipellis perniciosa; Filamentous fungi; Genomic DNA extraction; PCR

DNA isolation from some fungal organisms is difficult because they have cell walls or capsules that are relatively unsusceptible to lysis. Beginning with a yeast Saccharomyces cerevisiae genomic DNA isolation method, we developed a 30-min DNA isolation protocol for filamentous fungi by combining cell wall digestion with cell disruption by glass beads. High-quality DNA was ... more

S.C.O. Melo; C. Pungartnik; J.C.M. Cascardo; M. Brendel

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