Research Note

Related GMR Articles

Cattle; Effective population size; Inbreeding; Pigs; Sheep; Software

Genetic variation provides a basis upon which populations can be genetically improved. Management of animal genetic resources in order to minimize loss of genetic diversity both within and across breeds has recently received attention at different levels, e.g., breed, national and international levels. A major need for sustainable improvement and conservation programs is accurate ... more

E. Groeneveld; Bv.d. Westhuizen; A. Maiwashe; F. Voordewind; J.B.S. Ferraz
Pigs; RNA purification; Slaughterhouse

Since RNA extraction is a crucial step in many molecular techniques, the protocols for sample collection and RNA purification need to be adapted to optimize their performance when samples are collected from animals at commercial facilities. Here we provide an RNA purification protocol for animal tissues collected from slaughterhouses. This protocol, modified from other techniques, uses ... more

V. Méndez; E. Avelar; A. Morales; M. Cervantes; A. Araiza; D. González
Adipocyte fatty acid-binding protein; Gene expression; Longissimus dorsi; meat quality; Pigs; Polymorphism

Recent attention in pig breeding programs has focused on the improvement of pork quality in response to increasing consumer demands. Compared to the fatty-type Northeastern Indigenous (Chinese) breed of pigs, the lean-type Large White has lower intramuscular fat and inferior eating quality from the perspective of the Chinese consumer. In order to investigate the molecular basis of ... more

Y. Gao; Y.H. Zhang; H. Jiang; S.Q. Xiao; S. Wang; Q. Ma; G.J. Sun; F.J. Li; Q. Deng; L.S. Dai; Z.H. Zhao; X.S. Cui; S.M. Zhang; D.F. Liu; J.B. Zhang
Free amino acids; Gene expression; performance; Pigs; Protein-bound

Free amino acids (AA) appear to be absorbed faster than protein-bound AA (PB-AA). We conducted an experiment to assess the effect of feeding pigs with a partially free (F-AA) or totally PB-AA diet on expression of selected genes and performance of pigs. The expression of cationic AA transporters b0,+ and CAT-1 in intestinal mucosa, liver, and longissimus (LM) and ... more

A. Morales; F. Grageola; H. García; A. Araiza; R.T. Zijlstra; M. Cervantes
ANGPTL4; Association analysis; Differential expression; Intramuscular fat; Pigs

In a previous study, we investigated differences in gene expression in backfat between Meishan and Large White pigs and their F1 hybrids, Large White x Meishan, and Meishan x Large White pigs. One potential differentially expressed sequence tag from the mRNA differential display was a homolog of the human angiopoietin-like 4 (ANGPTL4) gene, which encodes a protein that ... more

Z.Q. Ren; W.J. Wu; W.H. Liu; R. Zheng; J.L. Li; B. Zuo; D.Q. Xu; F.E. Li; M.G. Lei; D.B. Ni; Y.Z. Xiong
Hepatitis E virus; Liver; Pigs; Reverse transcription-polymerase chain reaction; Zoonosis

Hepatitis E is a form of endemic acute hepatitis found in humans in many countries worldwide and is caused by the hepatitis E Virus (HEV). Detection of HEV in pigs indicates that they may be carriers, possibly through zoonosis. The prevalence of HEV in pigs in Colombia is unknown. Studies in the US found that 11% of pig livers sold in grocery stores are contaminated with HEV. It is also ... more

C. Gutiérrez-Vergara; J. Quintero; J.F. Duarte; J.P. Suescún; A. López-Herrera
Gene expression; Leu:Lys ratio; Pigs; Serum amino acids

We examined 96 pigs (28.1 ± 0.83 kg) to analyze the effect of Leu:Lys ratios on expression of the cationic amino acid transporters b0,+ and CAT-1 in the jejunum and liver as well as myosin expression in 2 muscles to estimate the optimum standardized ileal digestible (SID) Leu:Lys ratio for growth rate and efficiency. A wheat-and wheat bran-based diets were formulated to meet ... more

H. García; A. Morales; A. Araiza; J.K. Htoo; M. Cervantes
Brangus; Carcass; DNA; Growth; Growth hormone; SNP

Sequence polymorphisms in the growth hormone (GH) gene and its transcriptional regulators, Pit-1 and Prop-1, were evaluated for associations with growth and carcass traits in two populations of Brangus bulls Chihuahuan Desert Rangeland Research Center (CDRRC, N = 248 from 14 sires) and a cooperating breeding program (COOP, N = 186 from 34 sires). Polymorphisms were SNP mutations in intron 4 (C ... more

M.G. Thomas; R.M. Enns; K.L. Shirley; M.D. Garcia; A.J. Garrett; G.A. Silver
Dog breeds; Growth; Polymorphism; SNP; SOCS2

Suppressor of cytokine signaling 2 (SOCS2) is a negative regulator of growth hormone signaling. The deletion of SOCS2 in mice results in a 30-50% increase in post-natal growth. In an effort to identify polymorphisms in the SOCS2 gene that may be associated with body size in dogs, we characterized the canine SOCS2 gene and analyzed its genetic diversity among small and large dog breeds ... more

G. Rincón; A.E. Young; D.L. Bannasch; J.F. Medrano
Bos taurus; Carcass traits; Chemerin gene; PCR-SSCP; SNP

Qinchuan is a red or yellow draft and beef breed in China. In order to identify a predictor of carcass traits on the basis of associations between carcass traits and gene polymorphism, variation in the bovine chemerin gene was investigated using PCR-single-strand conformational polymorphism and DNA sequencing. An SNP of A868G located in exon 2 of the Bos taurus chemerin gene was ... more

W.Q. Tian; H.C. Wang; F.B. Song; L.S. Zan; H. Wang; H.B. Wang; Y.P. Xin; J.A. Ujan