Research Article

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A pair of inverted repeated sequences of the gene survivin was designed for stable double-stranded RNA establishment. After stable transfection, the biological behaviors of gastric cancer cells were observed. The interference rates of survivin-targeting siRNA (siRNA-survivin) in BGC823, MKN45, SGC7901, and cisplatin-resistant SGC7901 groups were 55.363 ± 3.974 ... more

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Acrylonitrile; Apoptosis; Brain neuron; Neuron morphology; Rat

This study aimed to evaluate the effects of acrylonitrile (ACN) on neuronal morphology and apoptosis in rats. An ACN solution was administered to Wistar rats by gavage at doses of 0, 5, 10, or 20 mg/kg, 5 days a week for 13 weeks. The morphology of neurons and the presence of apoptosis was examined by light and electron microscope, DNA electrophoresis, immunohistochemistry, and terminal ... more

C.Z. Li; L.A. Tse; Y.L. Zhou; W. Fan; M. Li; I.T.S. Yu; T.Y. Jin
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Apoptosis; Guanine nucleotide-binding protein; Pig; Thyroid gland

The Alpha subunit of the stimulatory guanine nucleotide-binding protein (GNAS) is a complex imprinted gene. The major product of the GNAS gene is the α-subunit of the guanine nucleotide-binding protein (Gas), which plays a key role in multiple signal transduction pathways. Gas is required for the production of the receptor-stimulated intracellular cyclic adenosine monophosphate ( ... more

J. Peng; Y.L. Li; Y.F. Shen; Y. Wang; N.Y. Xu
05/30/2014
Apoptosis; Fas/FasL; Severe aplastic anemia

Fas/FasL protein expression of bone marrow hematopoietic cells was investigated in severe aplastic anemia (SAA) patients. Fas expression was evaluated in CD34+, GlycoA+, CD33+, and CD14+ cells labeled with monoclonal antibodies in newly diagnosed and remission SAA patients along with normal controls. FasL expression was evaluated in CD8+ ... more

C.Y. Liu; R. Fu; H.Q. Wang; L.J. Li; H. Liu; J. Guan; T. Wang; W.W. Qi; E.B. Ruan; W. Qu; G.J. Wang; H. Liu; Y.H. Wu; J. Song; L.M. Xing; Z.H. Shao
05/23/2014
Apoptosis; Bcl-2/Bax; Caspase-8; Fas/FasL; Nonalcoholic fatty liver disease

The aim of this study was to investigate the roles of Fas/FasL, Bcl-2/Bax, and Caspase-8 mRNA expressions in nonalcoholic fatty liver disease (NAFLD). The apoptosis percentage was measured by flow cytometry, the immunohistochemical assay was performed for the determination of Fas, FasL, Bcl-2, and Bax expressions, and a real-time polymerase chain reaction (PCR) assay was performed to ... more

C.P. Li; J.H. Li; S.Y. He; P. Li; X.L. Zhong
05/23/2014
Apoptosis; Cancer; Cytotoxicity; MCF-7; Saudi Arabia

This study investigated the in vitro anticancer activities of a total of 14 wild angiosperms collected in Saudi Arabia. The cytotoxic activity of each extract was assessed against human breast adenocarcinoma (MCF-7) cell lines by using the MTT assay. Among the plants screened, the potential cytotoxic activity exhibited by the extract of Lavandula dentata (Lamiaceae) was ... more

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02/13/2014
Apoptosis; Hela cells; Mechanism; Menispermum dauricum

Menispermum dauricum DC possesses a wide range of pharmacological effects. In this study, the mechanism of apoptosis induced by active components of M. dauricum was investigated in the human cervical carcinoma HeLa cell line. HeLa cells were treated with different M. dauricum concentrations over different time periods. The proliferation-inhibitory rate and ... more

J.Y. Wang; S. Sun; L. Liu; W.S. Yang
05/09/2014
Apoptosis; Bortezomib; NFkB pathway; Prostate cancer; Trichostatin A

Prostate cancer is one of the most common types of urological cancers. Despite the implementation of effective radiotherapy and chemotherapy methods, prostate cancer cells can still show resistance to treatment. In recent years, a combination of proteasome and histone deacetylase inhibitors has been used to treat various malignancies. In this study, we examined the cytotoxic and apoptotic ... more

I. Kiliccioglu; E. Konac; N. Varol; S. Gurocak; Y. Bilen
04/17/2014
A7r5 cells; Apoptosis; Caspase-3; Caspase-9; Low-frequency ultrasound

Ultrasound, a non-invasive therapy method, is a potential tool for medical applications, but its biological effects on vascular smooth muscle cells (VSMCs) have not been characterized. The aim of this study was to explore the effect and possible apoptotic mechanism of VSMCs that were induced by low-frequency ultrasound (LFU). Cell viability and apoptosis of A7r5 cells were evaluated after ... more

B. Zhang; H.S. Zhou; Q. Cheng; L. Lei; B. Hu
01/14/2014
Apoptosis; Granulosa cells; INHBB; miR-34a

During ovarian follicular growth and development, only a few follicles actually ovulate. Recently, it was found that follicular atresia is triggered by granulosa cell apoptosis, but the molecular mechanism of follicular atresia was not understood. Using flow cytometry, we found that miR-34a promotes granulosa cell apoptosis in pig ovarian follicles. In addition, inhibin beta B was found ... more

F. Tu; Z.X. Pan; Y. Yao; H.L. Liu; S.R. Liu; Z. Xie; Q.F. Li

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