Research Article

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02/06/2015
Apoptosis; Curcumin; Invasion; Melanoma; Migration; Proliferation

Malignant melanoma is a melanocytic tumor with a high potential of invasion and metastasis. Curcumin is extracted from Curcuma longa L.; curcumin has anti-tumor efficacy in multiple systemic malignancies. Here, we investigated the effect of curcumin on A375 human melanoma cells. A375 cells were cultivated, passaged, and treated with different concentrations of curcumin. We ... more

Y.P. Zhang; Y.Q. Li; Y.T. Lv; J.M. Wang
01/15/2015
Apoptosis; BPH-1 cells; Cell cycle; Croton membranaceus; Proliferation

Croton membranaceus aqueous root extract (CMARE) is among the widely used phytotherapeutics in Ghana for the management of benign prostatic hyperplasia (BPH) and prostate cancer. However, the mechanism of action of CMARE remains to be elucidated. This study aimed to establish whether apoptosis is involved in the antiproliferative effect of CMARE on human BPH-1 cells. We ... more

D.K. Afriyie; G.A. Asare; K. Bugyei; J. Lin; J. Peng; Z. Hong
04/02/2013
Apoptosis; Invasion; miR-181b; PC-3; Proliferation; Prostate cancer

We examined microRNA-181b (miRNA) expression in prostate cancer tissues and its effect on the prostate cancer cell line PC-3. Tissues from 27 cases of prostate cancer and 30 samples of normal human prostate were collected by surgical removal. Total miRNA was extracted, and the relative expression of miR-181b was quantified using RT-PCR. miR-181b ASO was transfected into prostate cancer PC ... more

L. He; H. Yao; L.H. Fan; L. Liu; S. Qiu; X. Li; J.P. Gao; C.Q. Hao
07/07/2014
Apoptosis; Aspirin; Proliferation; Thy-1; Wnt/β-catenin

Cancer stem cells have been found to play important roles in carcinoma. Although thy-1 has been identified as a potential stem cell marker of liver cancer, whether the Wnt/β-catenin signaling pathway plays an important role in regulating hepatocarcinoma proliferation and apoptosis mediated by thy-1 remains unknown. Our results showed that high thy-1 expression caused hepG2 cells ... more

B.Q. Cheng; Y. Jiang; Q. Zhu; W.G. Lin
02/10/2009
Apoptosis; DNA damage; genomic instability; Inflammation; Isocyanates; Lung cancer

Lungs comprise the primary organ exposed to environmental toxic chemicals, resulting in diverse respiratory ailments and other disorders, including carcinogenesis. Carcinogenesis is a multi-stage phenomenon, which involves a series of genetic alterations that begin with genomic instability provoked by certain factors such as inflammation and DNA damage and end with the development of cancer. ... more

P.K. Mishra; A. Bhargava; G.V. Raghuram; S. Gupta; S. Tiwari; R. Upadhyaya; S.K. Jain; K.K. Maudar
12/08/2011
Apoptosis; Arg72Pro; Karyorrhexis; Micronuclei; rs56275308; TP53

Polymorphisms in the TP53 gene codon 72 (Arg72Pro) influence apoptosis induction and DNA damage repair. We evaluated how variants of protein p53 (p53Arg and p53Pro) affect cell death and DNA damage repair by analyzing the frequencies of karyorrhexis and micronuclei. There were significant differences in the frequency of karyorrhexis between the three p53 genotypes (Arg/Arg, Arg/ ... more

L. Pereira; M.R.S. Carvalho; C.G. Fonseca; S.S.S. Lima; E.M.M. Cerqueira; W. Jorge; M.C.L. Castro
10/18/2011
Apoptosis; CDV; Fas receptor; RT-PCR; Vero cells

We evaluated the expression of the Fas receptor gene in Vero cells infected with the Lederle vaccine strain of canine distemper virus using RT-PCR. Vero cells were plated, and after being grown for 24 h in MEM with 5% FBS, 80-90% confluent monolayer cultures were infected with the virus. The cells were harvested at 3, 6, 9, and 15 h post-infection. Uninfected Vero cells were used as a ... more

H.L. Del Puerto; A.S. Martins; G.F. Braz; F. Alves; M.B. Heinemann; D.S. Rajão; F.C. Araújo; S.F. Martins; D.R. Nascimento; R.C. Leite; A.C. Vasconcelos
09/09/2011
Angiotensin-II; Anti-aging; Apoptosis; IGF-1; klotho gene; PTH

Inactivation of the klotho gene in mice causes serious systemic disorders, resembling human aging. However, at the molecular level, its action mechanisms are not well understood. The stimulatory or inhibitory effects of cis- and trans-regulatory factors on the klotho gene expression are also still unclear. We studied the effects of intra- and extracellular factors on ... more

K. Turan; P. Ata
08/05/2011
Apoptosis; HSIL; LSIL; Micronucleus

We evaluated micronucleus and apoptosis occurrence among women with normal smears and women with different kinds of cervical abnormalities, i.e., inflammatory processes and low- and high-grade squamous intraepithelial lesions (N = 12, N = 10 and N = 27, respectively). The sample included 59 women who were seen at a public medical service for cervical cancer prevention in Feira de Santana, ... more

G.M.A. Aires; J.R.C. Meireles; P.C. Oliveira; J.L. Oliveira; E.L. Araújo; B.C. Pires; E.S.A. Cruz; N.F. Jesus; C.A.B. Pereira; E.M.M. Cerqueira
06/07/2011
A20 gene; Apoptosis; High glucose; HUVECs; Monocyte homing

Diabetes mellitus causes vascular lesions and may ultimately lead to atherosclerosis. One of the earliest steps in the development of atherosclerotic lesions is the adhesion of monocytes to endothelial cells of the vessel wall. It is currently unknown whether zinc finger protein A20 is able to protect endothelial cells from injury caused by high levels of glucose and monocyte homing. In ... more

C.L. Hou; W. Zhang; Y. Wei; J.H. Mi; L. Li; Z.H. Zhou; W. Zeng; D.J. Ying

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