Research Article

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03/12/2014
Body measurement; LYRM1; Meat quality traits; Qinchuan cattle; Single nucleotide polymorphism

Body measurement and meat quality traits play important roles in the evaluation of productivity in cattle; they are influenced by genetic and environmental factors. Recent studies have shown that LYRM1 is a novel gene related to obesity and may influence fat deposition. We screened for new polymorphisms in the bovine LYRM1 gene and analyzed their association with body measurement and meat ... more

Y.K. Li; Y.F. Huangpu; J.B. Gao; N. Yang; C.Z. Fu; H.B. Wang; G. Cheng; L.S. Zan
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Body measurement traits; Meat quality traits; PRKAG2; Qinchuan cattle; Single nucleotide polymorphism

Body measurement and meat quality traits play important roles in the evaluation of productivity and economy in cattle, which are influenced by genes and environmental factors. PRKAG2, which encodes the γ2 regulatory subunit of AMPK, is associated with key metabolic pathways in muscle. We detected bovine PRKAG2 gene polymorphisms and analyzed their associations with body ... more

C.Z. Fu; W.Q. Tian; Y.K. Li; S.J. Wei; G. Cheng; H.B. Wang; L.S. Zan
03/31/2014
BMP-2; COL6A1; Genetic association; Ossification of the posterior longitudinal ligament; Single nucleotide polymorphism

COL6A1 and BMP-2 genes have been implicated in ossification of the posterior longitudinal ligament (OPLL) susceptibility in Japanese and Chinese Han populations. However, no study has yet investigated the DNA of unaffected family members of patients with OPLL. This study investigated differences in genetic polymorphisms of BMP-2 and COL6A1 between Korean patients with ... more

K.H. Kim; S.U. Kuh; J.Y. Park; S.J. Lee; H.S. Park; D.K. Chin; K.S. Kim; Y.E. Cho
09/16/2011
Bovine C/EBPa gene; Genetic polymorphism; Meat quality traits; PCR-SSCP

CCAAT/enhancer-binding protein alpha (C/EBPa) is an essential transcriptional factor regulating the differentiation of adipocytes. We report a novel single nucleotide polymorphism (C271A) of the C/EBPa gene in six indigenous Chinese cattle breeds using PCR-SSCP and DNA sequencing methods. Allele frequencies were investigated and evaluated by the χ2 test in 817 individuals; all ... more

H. Wang; L.S. Zan; H.B. Wang; F.B. Song
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Cattle; Meat quality traits; MYPN gene; PCR-RFLP; SNP

Myopalladin (MYPN) is a multifunctional protein that maintains sarcomeric integrity and regulates Z-line structure. It is an important candidate gene for meat quality selection through marker-assisted selection. Using PCR-RFLP technology, we discovered a single-nucleotide polymorphism (SNP) (A1795G in exon 9) of the MYPN gene. Allele frequencies of this SNP were investigated and evaluated ... more

Y. Jiao; L.S. Zan; Y.F. Liu; H.B. Wang; B.L. Guo
10/07/2014
Body measurement traits; Meat quality traits; Polymorphisms; Qinchuan cattle; Smoothened

Beef cattle breeding programs focus on improving important economic traits, including growth rates, and meat quantity and quality. Molecular marker-assisted selection based on genetic variation represents a potential method for breeding genetically improved livestock with better economic traits. Smoothened (SMO) protein is a signal transducer that contributes to the regulation of both ... more

Y.R. Zhang; Y.K. Li; C.Z. Fu; J.L. Wang; H.B. Wang; L.S. Zan
05/11/2015
Meat quality traits; Polymorphisms; Qinchuan cattle

Here, we detected 2 SNPs, A85C and T335C, that were located on the 3rd exon and the 3 untranslated regions of the bovine Osteocrin gene, respectively, using 413 Qinchuan cattle DNA samples. PCR-SSCP and DNA sequencing methods were specifically used. Three genotypes (AA, AC, and CC) were found at A85C; yet, only 2 genotypes (TC and CC) were found at T335C. Association analysis showed that ... more

Y.F. Huangfu; L.S. Zan; C. Adoligbe; H.B. Wang; H. Wang; J.B. Gao
03/30/2015
Fatty acid synthase gene; Meat quality traits; Polymorphism; Yak

Fatty acid synthase (FASN) is a key enzyme in fatty acid anabolism that plays an important role in the fat deposit of eukaryotic cells. Therefore, in this study, we detected 2 novel single-nucleotide polymorphisms (SNPs) in the FASN gene in 313 adult individuals of Datong yak using polymerase chain reaction-single strand conformation polymorphism and DNA sequencing techniques. SNP ... more

M. Chu; X.Y. Wu; X. Guo; J. Pei; F. Jiao; H.T. Fang; C.N. Liang; X.Z. Ding; P.J. Bao; P. Yan
2017 Jan 23
alpha-Defensins; Animals; Gene expression; Gene Transfer Techniques; Lactuca; Plants, Genetically Modified; Rabbits

Lettuce (Lactuca sativa L.) is an annual plant of the daisy family, Asteraceae, with high food and medicinal value. However, the crop is susceptible to several viruses that are transmitted by aphids and is highly vulnerable to post-harvest diseases, as well as insect and mammal pests and fungal and bacterial diseases. Here, the rabbit defensin gene NP-1 was transferred into lettuce by ... more

D. Song; X. Xiong; W.F. Tu; W. Yao; H.W. Liang; F.J. Chen; Z.Q. He
2/16/2017
Adipose tissue; Animals; Bone morphogenetic protein 2; Cell differentiation; Cells, Cultured; Collagen type I; Osteoblasts; Osteocalcin; Osteogenesis; Polyethyleneimine; Rabbits; Stem cells; Tissue engineering; Transfection

The aim of this study was to examine the efficiency of polyethylenimine-mediated transfection of the human bone morphogenetic protein-2 (BMP-2) gene into rabbit adipose-derived stem cells (ADSCs), and its effect on osteoblast differentiation. Adipose tissue was isolated from the necks of adult Japanese white rabbits and cultured in vitro to obtain ADSCs. Gene delivery of BMP-2 was mediated by ... more

Q.F. Wang; Y. Huang; G.C. He; H.S. Wang; Z.H. Chen; X.H. Cai; Y.H. Xie; Q. Liu

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