Research Article

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The testis-specific protein Y-encoded gene (TSPY) is a Y-specific gene present in variable copy number in many mammalian species, including cattle. We tested the applicability of the TSPY gene as a Y-specific marker to predict preimplantation embryo sex in Nelore (Bos indicus) cattle. Two blastomeres were removed from each embryo. A total of 36 single ... more

M.C.A. Carneiro; P.L. Takeuchi; A. Araújo; R.B. Lôbo; F.P. Elias; R.A. Vila; C.L. Miranda-Furtado; E.S. Ramos
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Bovine; Candidate genes; Fat deposition; SNP identification

Canchim is a composite cattle breed developed in Brazil for beef production. One of the breeding objectives is to increase fat deposition. QTLs for fat thickness and/or marbling have been reported on BTA4 and BTA14. The IGFBP3 and DDEF1 genes, mapped to BTA4 and BTA14, respectively, affect adipogenesis. We looked for SNPs in the IGFBP3 and DDEF1 genes that could be associated with backfat ... more

G.B. Veneroni; S.L. Meirelles; D.A. Grossi; G. Gasparin; A.M.G. Ibelli; P.C. Tizioto; H.N. Oliveira; M.M. Alencar; L.C.A. Regitano
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Candidate genes; Cattle; DGAT1; PCR-RFLP; Zebu

Recent reports identified DGAT1 (EC 2.3.1.20) harboring a lysine to alanine substitution (K232A) as a candidate gene with a strong effect on milk production traits. Our objective was to estimate the frequency of the DGAT1 K232A polymorphism in the main Zebu and Taurine breeds in Brazil as well as in Zebu x Taurine crossbreds as a potential QTL for marker-assisted selection ... more

G.A. Lacorte; M.A. Machado; M.L. Martinez; A.L. Campos; R.P. Maciel; R.S. Verneque; R.L. Teodoro; M.G.C.D. Peixoto; M.R.S. Carvalho; C.G. Fonseca
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Beef; Bovine; Genome; Livestock; Molecular markers; SNP

We analyzed single nucleotide polymorphisms in calpain, leptin, leptin receptor, and growth hormone receptor genes and their association with color, drip and cooking losses of longissimus muscle at 7, 14 and 21 days postmortem in 638 purebred Nellore bulls slaughtered between 22 and 26 months of age. Meat samples were vacuum-packed and aged at 4°C. The single nucleotide polymorphisms ... more

L.F.B. Pinto; J.B.S. Ferraz; V.B. Pedrosa; J.P. Eler; F.V. Meirelles; M.N. Bonin; F.M. Rezende; M.E. Carvalho; D.C. Cucco; R.C.G. Silva
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Candidate genes; casein; Molecular markers; Zebu cattle; κ-casein

Frequencies of κ-casein gene alleles were determined in 1316 animals from the Brazilian Bos indicus genetic groups (Sindhi cows, Gyr sires, Gyr cows, Guzerat sires, Guzerat cows, Nellore sires, and Gyr x Holstein crossbreds) by means of polymerase chain reaction-restriction fragment length polymorphism analysis using two independent restriction nucleases (Hinf I and HaeIII). ... more

A.L.S. Azevedo; C.S. Nascimento; R.S. Steinberg; M.R.S. Carvalho; M.G.C.D. Peixoto; R.L. Teodoro; R.S. Verneque; S.E.F. Guimarães; M.A. Machado
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Bovine; Comparative map; FISH; Gene assignment

Seven genes were assigned by molecular cytogenetic methods to bovine chromosome 5. To accomplish this, specific primers were either publicly available or were designed from highly conserved regions of the publicly available mammalian gene sequences. The identity of the amplified segments was verified by sequencing and alignment with the published sequences. The optimized primers that amplified ... more

M. De Donato; D.S. Gallagher; C. Lehn; C. Gill; J.F. Taylor
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Acridine orange test; Bovine; Conservation; DNA; Freeze-drying sperm; TUNEL

The ability to detect nuclear damage is an important tool for the development of sperm preservation methods. We used the acridine orange test (AOT) and the terminal deoxynucleotidyl transferase-mediated dUDP nick-end labeling (TUNEL) assay to assess the DNA status of sperm cells preserved with different lyophilization media. The AOT did not detect any differences between different ... more

C.F. Martins; M.N. Dode; S.N. Báo; R. Rumpf
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Bovine; EtBr; Fibroblast; mtDNA; OXPHOS

Bovine fetal fibroblast cells were treated with ethidium bromide at a low concentration for 15 passages in culture to determine its effect on mitochondrial DNA copy number and on cell metabolism. Mitochondrial membrane potential and lactate production were estimated in order to characterize cell metabolism. In addition, mitochondrial DNA ND5 in proportion to a nuclear gene (luteinizing hormone ... more

M.Roberto Chiaratti; F.Vieira Meirelles
03/03/2005
Assisted reproduction technology; Bovine; Fibroblasts; Transfection; Transgenic animals

An association of two techniques, nuclear transfer (NT), and transfection of somatic animal cells, has numerous potential applications and considerable impact, mainly in agriculture, medicine, pharmacy, and fundamental biology. In addition, somatic cell nuclear transfer is the most efficient alternative to produce large transgenic animals. We compared in vitro and in vivo ... more

L.T. Iguma; L.T. Iguma; E.O. Melo; M.M. Franco; I. Pivato; G.R. Vianna; R.V. Sousa; M.A.N. Dode; F.J.L. Aragão; E.L. Rech; E.L. Rech; R. Rumpf; S.F.C. Lisauskas
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Bovine; Contig; Cluster; Expressed sequence tag; Gene atlas; Transcriptional profiling

Genes whose products function in a common biological process are often co-regulated. When regulation occurs at the transcriptional level, co-expressed genes can be detected globally by expression arrays or by sequencing non-normalized cDNA libraries. We examined bovine gene expression in 27 tissues using non-normalized cDNA library sequencing. Contigs were generated from expressed ... more

O.M. Keane; N. Maqbool; A.F. McCulloch; J.C. McEwan; K.G. Dodds

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