Research Article

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05/11/2007
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Cassava roots have a low-protein content (0.7-2%). Amino acids such as lysine and methionine are also low, and some research reports have indicated the absence of methionine. The amino acid profiles of a common cassava cultivar and an interspecific hybrid, namely ICB 300, were determined using the computerized amino acid analyzer Hitachi L-8500. The interspecific hybrid has 10 times ... more

N.M.A. Nassar; M.V. Sousa
11/11/2006
Cassava; India; Plant breeding; Productivity

South America is responsible for about half of the cassava world production. In the 1970’s productivity of the crop on the continent was about 15 ton/ha, and dropped continuously until reaching 12 ton/ha in 2004. India’s productivity of cassava increased from 10 ton/ha in the 1970’s to 28 ton/ha in 2004. Brazil contributed significantly to improving cassava crops through the Instituto ... more

N.M.A. Nassar
03/24/2009
Cassava; In silico analysis; Inverse polymerase chain reaction; Pt2L4 glutamic acid-rich protein; Root promoter; Transient expression

Cassava is one of the most important tropical food crops for more than 600 million people worldwide. Transgenic technologies can be useful for increasing its nutritional value and its resistance to viral diseases and insect pests. However, tissue-specific promoters that guarantee correct expression of transgenes would be necessary. We used inverse polymerase chain reaction to isolate a ... more

C.R. de Souza; F.J. Aragão; E.C.O. Moreira; C.N.M. Costa; S.B. Nascimento; L.J. Carvalho
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Anatomy; Cassava; Diploid stems; Tetraploid stems

Information on anatomical structure is needed by breeders working on improvement for drought tolerance. For studying the effect of polyploidy on cassava anatomy and its significance to tolerance to drought, we induced a polyploidy type of a selected clone (UnB 530) by applying an aqueous solution of 0.2% colchicine on lateral buds for a period of 12 h. The stem identified as tetraploid was ... more

N.M.A. Nassar
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Cassava; Gene expression; Gene walking; promoters

Cassava can be cultivated on impoverished soils with minimum inputs, and its storage roots are a staple food for millions in Africa. However, these roots are low in bioavailable nutrients and in protein content, contain cyanogenic glycosides, and suffer from a very short post-harvest shelf-life, and the plant is susceptible to viral and bacterial diseases prevalent in Africa. The demand ... more

M.A. Gbadegesin; J.R. Beeching
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Transposable elements contribute to the size, structure, variation, and diversity of the genome and have major effects on gene function. Sequencing projects have revealed the diversity of transposable elements in many organisms and have shown that they constitute a high percentage of the genome. PCR-based techniques using degenerate primers designed from conserved enzyme domains of ... more

M.A. Gbadegesin; J.R. Beeching
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Cassava; Essential amino acids; Interspecific hybrids; Protein

Cassava (Manihot esculenta) is a principal food for large populations of poor people in the tropics and subtropics. Its edible roots are poor in protein and lack several essential amino acids. Interspecific hybrids may acquire high protein characteristics from wild species. We analyzed 19 hybrids of M. esculenta with its wild relative, M. oligantha, for crude ... more

P.T.C. Gomes; N.M.A. Nassar
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Cassava; M-AFLP; Manihot species; Microsatellites

We developed molecular markers for cassava based on the microsatellite-amplified fragment length polymorphism (M-AFLP) technique. Twenty primer pairs were developed and used for the analysis of 48 samples of Manihot species, consisting of M. esculenta (33), M. esculenta ssp flabellifolia (3), M. chlorosticta (3), M. carthaginensis (3 ... more

S. Whankaew; S. Sraphet; R. Thaikert; D.R. Smith; K. Triwitayakorn
07/28/2015
Cassava; Linkage group; Manihot esculenta; Molecular genetic map; Physical localization; Primed in situ labeling

Physical localization of molecular markers and assign­ment of the 15th linkage group to chromosome 11 of the karyotype in cassava (Manihot esculenta Crantz) were achieved using primed in situ labeling. Amplified signals for both the EST507-1 and SSRY13-5 markers were consistently observed in different stages of cell division. A comparison of the length, arm ratio, and ... more

Y. Wang; J.F. Wang; H. Yin; H.Q. Gao; N.S. Zhuang; J.P. Liu