Research Article

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08/16/2012
Barcoding; ITS2; Legumes; Mediterranean; rpoC1; trnL

The ability to discriminate all species is the ultimate target in barcoding. The Mediterranean basin is a center of origin for legumes and thus they have played a key role in feeding the Mediterranean population. It is also a region with important protected designation of origin and protected geographical indication legumes that provide income in rural areas. We evaluated the use of two ... more

P. Madesis; I. Ganopoulos; P. Ralli; A. Tsaftaris
09/27/2013
DNA barcoding; Fallopia multiflora (Thunb.) Harald.; ITS2; matK; psbA-trnH; rbcL

Fallopia multiflora, locally known as Heshouwu, is one of the most important and widely used Chinese medicinal herbs. However, there is still considerable confusion concerning its different provenances. DNA barcoding is a recent aid to taxonomic identification and uses a short standardized DNA region to discriminate plant species. We assessed the applicability of 4 candidate DNA ... more

X.Q. Sun; M.M. Bai; H. Yao; J.L. Guo; M.M. Li; Y.Y. Hang
04/16/2014
Cassia; DNA barcoding; ITS2; matK; rbcL; trnH-psbA

DNA barcoding is a desirable tool for medicinal product authentication. DNA barcoding is a method for species identification using short DNA sequences that are conserved within species, but variable between species. Unlike animals, there is no single universal DNA barcode locus for plants. Coding markers, matK and rbcL, and noncoding markers, trnH-psbA ( ... more

N. Purushothaman; S.G. Newmaster; S. Ragupathy; N. Stalin; D. Suresh; D.R. Arunraj; G. Gnanasekaran; S.L. Vassou; D. Narasimhan; M. Parani
12/08/2011
Amplification success; Barcoding; Birds; Cytochrome oxidase 1; Mammals; Mini-barcode; PCR; Reptiles

In recent years, DNA barcoding has emerged as a powerful tool for species identification. We report an extended validation of a universal DNA mini-barcode for amplification of 130-bp COI segments from 23 specimens collected from a desert environment, including 11 reptiles, five mammals and seven birds. Besides the standard double-annealing protocol, we also tested a more stringent single ... more

I.A. Arif; H.A. Khan; A. Sadoon; M. Shobrak
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Barcoding; Chloroplast genes; High-resolution melting; species identification; Uvaria sp

DNA barcoding, which was developed about a decade ago, relies on short, standardized regions of the genome to identify plant and animal species. This method can be used to not only identify known species but also to discover novel ones. Numerous sequences are stored in online databases worldwide. One of the ways to save cost and time (by omitting the sequencing step) in species ... more

M. Osathanunkul; P. Madesis; S. Ounjai; K. Pumiputavon; R. Somboonchai; P. Lithanatudom; T. Chaowasku; J. Wipasa; C. Suwannapoom; M. Osathanunkul; P. Madesis; S. Ounjai; K. Pumiputavon; R. Somboonchai; P. Lithanatudom; T. Chaowasku; J. Wipasa; C. Suwannapoom; M. Osathanunkul; P. Madesis; S. Ounjai; K. Pumiputavon; R. Somboonchai; P. Lithanatudom; T. Chaowasku; J. Wipasa; C. Suwannapoom
11/25/2015
Brassicaceae; ITS; ITS2; matK; rbcL; trnH-psbA

Brassicaceae is a large plant family of special interest; it includes many economically important crops, herbs, and ornamentals, as well as model organisms. The taxonomy of the Brassicaceae has long been controversial because of the poorly delimited generic boundaries and artificially circumscribed tribes. Despite great effort to delimitate species and reconstruct the phylogeny of Brassicaceae ... more

X.Q. Sun; Y.Q. Qu; H. Yao; Y.M. Zhang; Q.Q. Yan; Y.Y. Hang
11/26/2012
AMOVA; Arecaceae; Genetic resources; RAPD

Oenocarpus mapora is an Amazonian palm species commonly used by native populations for food and in folk medicine. We measured genetic variability, using RAPD markers, of material kept in a germplasm bank composed of accessions sampled from the Brazilian Amazon. These included 74 individuals from 23 accessions sampled from 9 localities in three States of the Brazilian Amazon. ... more

E.F. Moura; M.S.P. de Oliveira
12/19/2016
Arecaceae; Genetics, Population; Polymorphism, Genetic; Population Density; Seedlings; Waxes

The objective of this study was to assess the spatial genetic structure (SGS) at different life stages (cohorts) in a remnant population (N = 101) of Copernicia prunifera in the semiarid region of northeastern Brazil. Using seven inter-simple sequence repeat molecular markers, we were able to analyze 93 loci with 100% polymorphism. Seedlings had the highest level of genetic diversity (H = 0. ... more

F.A. Vieira; R.F. Sousa; C.G. Fajardo; M.M. Brandão
06/29/2017
Arecaceae; DNA Primers; Endangered species; Genetic markers; Heterozygote; Microsatellite Repeats; Polymerase chain reaction; Polymorphism, Genetic

The licuri palm Syagrus coronata plays a key role in the ecology and economy of Brazilian semiarid region. Nonetheless, genetic data about populations of this species are absent even though the intensive and uncontrolled exploitation since colonial periods has threatened the sustainability and viability of licuri populations. Therefore, we attempted to test the efficacy of transferability of ... more

R.R. Simplicio; D.G. Pereira; A.M. Waldschmidt
08/31/2017
Arecaceae; Evolution, Molecular; Gene flow; Gene frequency; Heterozygote; Linkage disequilibrium; Microsatellite Repeats; Polymorphism, Genetic

We assessed the level and distribution of genetic diversity in three species of the economically important palm genus Astrocaryum located in Pará State, in northern Brazil. Samples were collected in three municipalities for Astrocaryum aculeatum: Belterra, Santarém, and Terra Santa; and in two municipalities for both A. murumuru: Belém and Santo Antônio do Tauá and A. paramaca: Belém and ... more

N.P. Oliveira; M.S.P. Oliveira; L.C. Davide; S. Kalisz

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