Research Article

Related GMR Articles

10/19/2011
Genotyping; Heterozygote deficiency; Microsatellite; Null alleles; Parentage; White-tailed deer

Microsatellites are useful tools for ecological studies because they can be used to discern population structure, dispersal patterns and genetic relationships among individuals. However, they can also yield inaccurate genotypes that, in turn, bias results, promote biological misinterpretations, and create repercussions for population management and conservation programs. We used empirical ... more

A.C. Kelly; N.E. Mateus-Pinilla; M. Douglas; M. Douglas; P. Shelton; J. Novakofski
05/17/2011
Enriched genomic library; Epinephelus fuscoguttatus; Grouper; Microsatellite

Epinephelus fuscoguttatus is a commercially important marine fish species in southeast Asia. Due to overfishing and water pollution, this species has been declared as near-threatened. Thus, to provide information to help maintain and preserve the species, microsatellites were developed, using an enriched genomic library method. Thirty individuals were collected from the hatchery ... more

M.A.A. Mokhtar; M.N. Normah; S.V. Kumar; S.N. Baharum
05/10/2011
Abalone; Aquaculture; Haliotis diversicolor; Microsatellite

The abalone, Haliotis diversicolor, is one of the most important mariculture species in southern China. We developed 60 new polymorphic microsatellite markers for H. diversicolor and characterized them in 30 individuals from a cultured population in Sanya, China. All 60 markers were found to be polymorphic. The number of alleles ranged from two to nine per locus, with an ... more

Y. Wang; F. Wang; Y.H. Shi; Z.F. Gu; A.M. Wang
09/08/2010
Cerrado; Loricariidae; Microsatellite; Shotgun library

We isolated and characterized 10 microsatellite loci in the armored catfish (Hypostomus gymnorhynchus, Loricariidae), using a genomic shotgun library to obtain the repetitive sequences. Twenty-four primers were designed and 14 individuals of H. gymnorhynchus from the Caiapó River, in central Brazil, were genotyped using these primers to analyze the polymorphism at each ... more

M.P.C. Telles; L.V. Resende; R.P.V. Brondani; R.G. Collevatti; M.C. Costa; N.J.Silva Júnior
08/03/2010
Cerrado; Erythrinidae; Microsatellite; Shotgun library

We identified 14 microsatellite loci for the wolf fish, Hoplias malabaricus (Erythrinidae), from a genomic shotgun library. Twenty-five primers were designed, and 48 individuals of H. malabaricus from four localities of northwest Goiás, in central Brazil, were genotyped to characterize the polymorphism at each locus. Fourteen primers amplified clearly interpretable ... more

S.G.C.A. Gondim; L.V. Resende; R.P.V. Brondani; R.G. Collevatti; N.J. Silva-Júnior; R.R. Pereira; M.P.C. Telles
05/11/2010
BMP4 gene; Body measurement traits; Cattle; Microsatellite

We examined the variation of the BMP4 gene in four Chinese indigenous cattle breeds and investigated the association of this polymorphism with body measurement traits. Using PCR-SSCP and DNA sequencing, a polymorphic microsatellite was detected in the third exon of the bovine BMP4 gene in 459 samples from four Chinese indigenous cattle breeds, Qinchuan, Luxi, Nanyang, ... more

X. Zhong; L.S. Zan; H.B. Wang; Y.F. Liu
03/16/2010
Chelonian; Irapuca; Mating system; Microsatellite; Paternity; Podocnemis erythrocephala

The genus Podocnemis comprises six living species, including P. erythrocephala (irapuca - red-headed river turtle). Data are available concerning the reproductive biology of the species of the genus Podocnemis, but little is known about their reproductive strategies. Considering the total lack of such data for P. erythrocephala, and with the goal of ... more

C. Fantin; I.P. Farias; L.A.S. Monjeló; T. Hrbek
02/18/2010
Brocket deer; Heterologous primers; Mazama; Microsatellite

Transferability of microsatellite loci between closely related species has been reported in several species. This helps reduce costs involved with the development of primers for newly investigated species. Fifteen microsatellite primers developed for Rangifer tarandus, Cervus elaphus, C. axis, and Moschus berezovskii were tested on five species of ... more

A.M.B. Mantellatto; E.A.G. Carnelossi; J.M.B. Duarte
12/04/2013
Cynoglossus semilaevis; Genetic variability; Microsatellite; Multiplex assays; Tongue sole

The tongue sole, Cynoglossus semilaevis (Cynoglossidae), is one of the most economically important fishery resources in Korea. This study presents a preliminary investigation of the future viability of the complete aquaculture of tongue sole in Korea. Specifically, possible differences in genetic variability between wild populations of tongue sole from Korea and hatchery-produced ... more

H.S. An; E.M. Kim; H.W. Kang; H.S. Han; J.W. Lee; J.Y. Park; J.I. Myeong; C.M. An
11/22/2013
Diversity; Expressed sequence tags; Hevea brasiliensis; Microsatellite

Cultivated clones of Hevea brasiliensis have a narrow genetic base. In order to broaden the genetic base, it is first necessary to investigate the genetic diversity of wild populations. Expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed to investigate the genetic diversity of Hevea populations. Four hundred and thirty microsatellites were ... more

Z.W. An; Y.C. Li; Q.L. Zhai; L.L. Xie; Y.H. Zhao; H.S. Huang

Pages