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Related GMR Articles

02/27/2014
Armeniaca vulgaris; DNA extraction; Malus sieversii; Polymerase chain reaction; Vitis vinifera

Leaves of Malus sieversii, Vitis vinifera, and Armeniaca vulgaris contain substantial amounts of secondary metabolites, which limit the high-quality DNA extraction performance. In this study, five extraction protocols were compared for their ability to produce good quality DNA from fresh and dried (with silica gel) leaves of these species. The modified protocol ... more

K. Aubakirova; M. Omasheva; N. Ryabushkina; T. Tazhibaev; G. Kampitova; N. Galiakparov
06/11/2015
DNA extraction; Half-seed; Oryza sativa L.; Polymerase chain reaction; Pre-germination genotyping

Genotyping is a critical step for molecular marker-assisted selection in rice. Rice genomic DNA samples for genotyping are typically isolated from living tissues such as seedlings. This requires the germination of all candidate seeds and extraction of DNA from the seedlings. Currently, an ideal individual is selected from a very large number of plants, which is time- and labor-consuming ... more

Y.B. Duan; F.L. Zhao; H.D. Chen; H. Li; D.H. Ni; P.C. Wei; W. Sheng; J.T. Teng; A.M. Zhang; J.P. Xue
05/25/2015
Cut-and-paste-based cloning strategy; p150Glued; Polymerase chain reaction; Site-directed mutagenesis

Site-directed mutagenesis is an essential technique for investigating the mechanisms of gene regulation on a molecular level, as well as for exploring post-translational modifications and functional structure at the protein level. Polymerase chain reaction combining in vitro synthesis of oligonucleotide primers allows for site-directed mutation to be performed with ease. However, site-directed ... more

C. Wang; T.Y. Wang; L.Y. Zhang; X.J. Gao; X.W. Wang; C.J. Jin
01/30/2015
Extraction efficiency; Freshwater; Microbial diversity; Polymerase chain reaction; Total DNA

The demand for molecular analysis of aquatic microbial communities in freshwater has highlighted the need for efficient methods of DNA extraction. The centrifugation method and filtration-membrane method are 2 widely used methods for extracting DNA. The objective of this study was to compare the extraction efficiency of 3 methods, including the centrifugation method, filtration-membrane ... more

P. Li; S.F. Yang; B.B. Lv; K. Zhao; M.F. Lin; S. Zhou; X. Song; X.M. Tang
01/30/2015
Arachis hypogaea; Black aspergilla; Loop-mediated isothermal amplification; Ochratoxins; Polymerase chain reaction

Over the last decade, ochratoxin A (OTA) has been widely described and is ubiquitous in several agricultural products. Ochratoxins represent the second-most important mycotoxin group after aflatoxins. A total of 34 samples were surveyed from 3 locations, including Mecca, Madina, and Riyadh, Saudi Arabia, during 2012. Fungal contamination frequency was determined for surface-sterilized ... more

H.M. Al-Sheikh
03/29/2011
DNA extraction; PCR; Protocol

Conventional genomic DNA extraction protocols need expensive and hazardous reagents for decontamination of phenolic compounds from the extracts and are only suited for certain types of tissue. We developed a simple, time-saving and cost-efficient method for genomic DNA extraction from various types of organisms, using relatively innocuous reagents. The protocol employs a single ... more

T.Y. Wang; L. Wang; J.H. Zhang; W.H. Dong
08/17/2010
DNA; Equine; Lung; PBMC; Protocol

We compared three different protocols for DNA extraction from horse peripheral blood mononuclear cells (PBMC) and lung fragments, determining average final DNA concentration, purity, percentage of PCR amplification using β-actin, and cost. Thirty-four samples from PBMC, and 33 samples from lung fragments were submitted to DNA extraction by three different protocols. Protocol A consisted ... more

E.M. Santos; J.F.R. Paula; P.M.C. Motta; M.B. Heinemann; R.C. Leite; J.P.A. Haddad; H.L. Del Puerto; J.K.P. Reis

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