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Related GMR Articles

03/19/2012
Animal skins; DNA isolation; Leather

Sometimes, commercial products obtained from wild animals are sold as if they were from domestic animals and vice versa. At this point of the productive chain, legal control of possible wildlife products is difficult. Common in the commerce of northern Argentina, skins of two wild species, the carpincho and the collared peccary, look very similar to each other and to those of the domestic ... more

G.N. Ojeda; P.S. Amavet; E.C. Rueda; P.A. Siroski
02/13/2012
Artemisia monosperma; Citrullus colocynthis; DNA isolation; Restriction endonuclease digestion

Medicinal plant species has a valuable economic importance because of its usage as pharmaceuticals, nutritional, as well as its use in popular medication. For DNA-based techniques, nanogram quantities of the purified DNA are requisite to amplify and yield sufficient amounts of PCR products. SDS-based DNA isolation method was used to extract DNA from 11 species of different aromatic and ... more

A.A. Alatar; M.A. Mahmoud; S.A. Al-Sohaibani; K.A. Abd-Elsalam
09/30/2007
Conservation; Leguminosae; Molecular variation; Polymerase chain reaction

Dalbergia nigra (rosewood) is a long-lived leguminous species, which is endemic to the Brazilian Atlantic forest. Because of the high economic value of its wood, this species has been over-explored in recent years. Currently, rosewood is included in the IUCN Red List as vulnerable. We examined the genetic diversity of 87 specimens of D. nigra sampled from a continuous ... more

F.S. Juchum; J.B. Leal; L.M. Santos; M.P. Almeida; D. Ahnert; R.X. Corrêa
11/02/2008
Atlantic rainforest; Lysis; Microbial DNA; Polymerase chain reaction; Soil

The need for the prospecting for and identification of new biomolecules is a reality. Molecular techniques allow access to the metabolic potential of microorganisms via the isolation of DNA from environmental samples, i.e., without the application of microbial culture techniques. With its great biological diversity, the Atlantic Rainforest biome has a soil rich in organic matter, some ... more

J.H. Amorim; T.N.S. Macena; G.V. Lacerda-Junior; R.P. Rezende; J.C.T. Dias; M. Brendel; J.C.M. Cascardo
08/05/2011
DNA; Ladder; Polymerase chain reaction; λ DNA

Serving as a DNA molecular weight standard, the DNA ladder has been widely used in molecular biology applications. We developed a simple method for the preparation of a DNA marker, which involves designing primers to amplify 100- to 1000-bp DNA fragments using lambda DNA as a template for polymerase chain reaction, followed by extraction with phenol/chloroform, precipitation with ethanol ... more

T.Y. Wang; L. Wang; F. Wang
02/02/2010
Filamentous fungi; Fusarium oxysporum; Genomic DNA extraction; Polymerase chain reaction; Pyrenochaeta terrestris

DNA isolation from some fungal organisms of agronomic importance is difficult because they have cell walls or capsules that are relatively unsusceptible to lysis. We have developed a fast DNA isolation protocol for Fusarium oxysporum, which causes fusarium wilt disease in more than 100 plant species, and for Pyrenochaeta terrestris, which causes pink root in onions. This ... more

D. González-Mendoza; R. Argumedo-Delira; A. Morales-Trejo; A. Pulido-Herrera; L. Cervantes-Díaz; O. Grimaldo-Juarez; A. Alarcón
02/19/2013
Epidemiology; Papillomavirus infection; Polymerase chain reaction

Human papillomavirus (HPV) infection is a common viral sexually transmitted infection and the main cause of cervical cancer in women worldwide. Epidemiological data on the prevalence of HPV in a given population is essential for the establishment of effective prevention strategies. The aim of this study was to determine HPV prevalence in women who attended a public health service within ... more

J. Coser; daRocha Boeira; D. Simon; A.S.Kazantzi Fonseca; N. Ikuta; V.R. Lunge
09/27/2013
Genomic DNA extraction; Polymerase chain reaction; Prosopis spp

The extraction of high-quality genomic DNA from Prosopis spp for polymerase chain reaction (PCR) amplification is complicated, owing to the presence of a high percentage of secondary metabolites that bind to or co-precipitate with nucleic acids. In the present study, we report a modified sodium dodecyl sulfate/phenol protocol that eliminates the use of liquid nitrogen in the ... more

C.Y. Michel-López; D. González-Mendoza; O. Grimaldo-Juarez
12/17/2012
Amazon; Epstein-Barr; Gastric cancer; Polymerase chain reaction; Resection margin

Gastric cancer is one of most frequent causes of death in Brazil. The city of Manaus has one of the highest incidences of this disease in Brazil. The Epstein-Barr virus (EBV) is a ubiquitous herpesvirus that is classified as a group 1 carcinogen by the International Agency for Research on Cancer. We obtained biopsies from 6 control subjects and 10 patients with gastric carcinomas living ... more

P.F. de Aquino; P.C. Carvalho; J.Sda Gama Fischer; A.Q.L. de Souza; J.S. Viana; S.R.S. Chalub; A.D.L. de Souza; M.G.C. Carvalho
05/08/2012
Genetic identity; Polymerase chain reaction; Simple sequence repeat; Sugarcane

Red rod is an economically important disease of sugarcane caused by the fungus Colletotrichum falcatum. We used a simple sequence repeat (SSR)-based marker system to identify and analyze genetic relationships of red rot resistant and susceptible sugarcane cultivars grown in Pakistan. Twenty-one highly polymorphic SSR markers were used for DNA fingerprinting and genetic diversity ... more

U. Hameed; Y.B. Pan; K. Muhammad; S. Afghan; J. Iqbal

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