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Related GMR Articles

DNA isolation; Fungi; Lyticase; Proteinase K

DNA isolation is a crucial step of conducting genetic studies in any organism. However, this process is quite difficult when studying fungi because of the need to damage the fungal cell walls of specific structures. In this study, we developed a method for the rapid and efficient isolation of fungal DNA based on simultaneous mechanical and enzymatic cell wall degradation. There are ... more

T. Lech; J. Syguła-Cholewinska; J. Szostak-Kot
Genetic diversity; Polymerase chain reaction; Random amplified polymorphic DNA; Sugarcane

Genetic diversity of 20 sugarcane accessions in Pakistan was studied using 21 random amplified polymorphic DNA markers. The mean genetic distance between the cultivars was 39.03%, demonstrating that a large part of the genome is similar among the accessions. This probably arises from a lack of parental diversity, with few clones, which are themselves related, contributing to the parentage ... more

F.A. Khan; A. Khan; F.M. Azhar; S. Rauf
Leptin; obese gene; Polymerase chain reaction; Quantitative trait loci; Restriction fragment length polymorphism

The aim of the present study was to identify polymorphisms in the leptin gene of 112 pigs and compare the maternal and paternal lineage of Pietrain and Large White breeds that underwent a divergent selection for over 30 years. DNA samples extracted from the blood of these animals were amplified by polymerase chain reaction and genotyped by restriction fragment length polymorphism ... more

A.C.P. Silveira; R.C. Antunes; J.F. Almeida; T.F. Braga; P.F.A. Freitas; A.S.M. César; A.S.M. César; E.C. Guimarães
Cathechol-o-methyltransferase; Dopamine D3 receptor; Polymerase chain reaction; Restriction fragment length polymorphism; Schizophrenia; Single nucleotide polymorphism

Molecular components of the dopamine D3 receptor (DRD3) may play an important role in the pathophysiology of schizophrenia. Previous studies have demonstrated an association between DRD3 Ser9Gly and cathechol-o-methyltransferase (COMT, SNP = rs165656) polymorphisms and schizophrenia but the results were inconclusive. We investigated this apparent association between Ser9Gly (A/G) ... more

S.F. Tee; P.Y. Tang; H.C. Loh
Chickpea; Genetic diversity; Polymerase chain reaction; Random amplified polymorphic DNA

Genetic diversity analysis of chickpea germplasm can provide practical information for the selection of parental material and thus assist in planning breeding strategies. Chickpea seed is a good source of carbohydrates and proteins, constituting 80% of the total dry seed weight. Released cultivars and advanced lines of 30 chickpea genotypes were subjected to RAPD analysis for assessment ... more

F. Ahmad; A.I. Khan; F.S. Awan; B. Sadia; H.A. Sadaqat; S. Bahadur
Human papillomavirus; Polymerase chain reaction; Risk factors; Uterine cervical neoplasms

We examined the prevalence of human papillomavirus (HPV) infection in Brazilian women with cervical intraepithelial neoplasia. Our goal was to identify the types of HPV and their association with risk factors. This prospective cross-sectional study included 97 samples collected from women aged 14-79 years at the public health units of gynecological care in São Luís, MA, Brazil. HPV ... more

J.D.C. Nunes; F.C.B. Vidal; C.T.L. Ferraro; M.B.C. Chein; L.M.O. Brito; S.C.M. Monteiro
25S rDNA-PCR; Candida albicans; Polymerase chain reaction; Random amplified polymorphic DNA

We analyzed the distribution of Candida albicans in the oral cavity of 3-5-year-old children of Uygur and Han nationalities as well as their genotypes in caries-active groups in the Urumqi municipality. CHROMagar Candida was separately cultivated, and we identified 359 Uygur and Han children aged 3-5 years. We randomly selected 20 Han children and 20 Uygur children for this ... more

N. Wu; J. Lin; L. Wu; J. Zhao
CENP-B gene; CENP-B protein; Centromere formation; DNA isolation; Vegetal species

To explore the CENP-B centromere protein in beans, carrots, onions and potatoes, total RNA was isolated and reverse transcribed by PCR, and the cDNA encoding the CENP-B amino terminus domain amplified using CENP-B oligonucleotides. Blots containing PCR products were hybridized with a nick-translated pG/CNPB probe containing a complete human CENP-B gene. In all the plant species, anti- ... more

O. Barbosa-Cisneros; R. Herrera-Esparza
Bark; Cambium; DNA isolation; Leguminosae; Trees; Trunk

Traditionally, molecular studies of plant species have used leaves as the source of DNA. However, sampling leaves from tall tree species can be quite difficult and expensive. We developed a sequence of procedures for using stem bark as a source of DNA from Leguminosae trees of the Atlantic Forest and the Cerrado. Leguminosae is an important species-rich family in these two highly diverse and ... more

R.M.L. Novaes; J.G. Rodrigues; M.B. Lovato
DNA isolation; Isoamylase; Starch; Wheat (Triticum aestivum L.)

In higher plants, isoamylase-type starch debranching enzyme catalyzes the α-1,6-glucosidic linkages of glycogen and phytoglycogen. We cloned an isoamylase-type starch debranching enzyme ISA3 cDNA sequence (2883 bp), designated as TaISA3, from common wheat (Triticum aestivum), using the rapid amplification of cDNA ends method. The open reading frame of TaISA3 was ... more

G.Z. Kang; G.Q. Liu; W. Xu; Y.J. Zhu; C.Y. Wang; H.Q. Ling; T.C. Guo