Research Article

Related GMR Articles

Canavalia brasiliensis lectin; Heterologous; Pichia pastoris; Protein splicing; Recombinant

During its biosynthesis in developing Canavalia brasiliensis seeds, the lectin ConBr undergoes a form of protein splicing in which the order of the N- and C-domains of the protein is reversed. To investigate whether these events can occur in other eukaryotic organisms, an expression system based on Pichia pastoris cells was established. A DNA fragment encoding prepro-ConBr was ... more

W.Melgaço Bezerra; C.Paiva da S. Carvalho; Rde Azevedo Moreira; T.Barbosa Grangeiro
Cysteine peptidase; Pichia pastoris; Recombinant expression; Xanthomonas citri subsp citri

Xanthomonas citri subsp citri (Xac) is the bacterium responsible for citrus canker disease in citrus plants. The aim of this study was to describe the recombinant expression, purification, and characterization of a cysteine peptidase from Xac strain 306, which is a candidate for involvement in the pathogenicity of this bacterium. The gene was cloned and ... more

A. Soares-Costa; R.S. Silveira; M.T.M. Novo; M.F.M. Alves; A.K. Carmona; J. Belasque; F. Henrique-Silva
Bovine papillomavirus; L1 gene; Pichia pastoris

Papillomaviruses are known to cause benign or malignant lesions in various animals. In cattle, bovine papillomavirus (BPV) is the etiologic agent of papillomatosis and neoplasia of the upper gastrointestinal tract and urinary bladder. Currently, there are no standard diagnostic tests or prophylactic vaccines. Protection against papillomavirus infection is conferred by neutralizing ... more

A.L.S. Jesus; F.C. Mariz; H.M. Souza; M.N. Cordeiro; E.C. Coimbra; M.C.G. Leitão; L.M. Nascimento; R.C. Stocco; W. Beçak; A.C. Freitas
Aralia elata; Pichia pastoris; PNY1; Real-time RT-PCR; Triterpenoid saponins; β-amyrin synthase

Aralia elata is an important medicinal plant in China; it produces large amounts of oleanane type triterpene saponins. A full-length cDNA encoding β-amyrin synthase (designated as AeAS) was isolated from young leaves of A. elata by reverse transcription-PCR. The full-length cDNA of AeAS was found to have a 2292-bp open reading frame, encoding a ... more

Y. Wu; H.D. Zou; H. Cheng; C.Y. Zhao; L.F. Sun; S.Z. Su; S.P. Li; Y.P. Yuan
Pichia pastoris; Second-generation ethanol; Trichoderma harzianum

Filamentous fungi from the genus Trichoderma have been widely investigated due to their considerable production of important biotechnological enzymes. Previous studies have demonstrated that the T. harzianum strain IOC-3844 has a high degree of cellulolytic activity. After excluding the native signal peptide, the open reading frame of the T. harzianum ... more

W.C. Generoso; W. Malagó-Jr.; N. Pereira; F. Henrique-Silva
Collichthys lucidus; Cystatin C; Expression; Gene cloning; Quantitative real-time PCR

Cystatins are natural tight-binding reversible inhibitors of cysteine proteases. In this study, a cDNA library was constructed from Collichthys lucidus using the SMART technique. A complete cDNA sequence with high identity to the conserved sequence of the cystatin C gene was cloned from the library using EST analysis and rapid amplification of cDNA ends (RACE), then subjected to ... more

W. Song; K.J. Jiang; F.Y. Zhang; M. Zhao; L.B. Ma; W. Song; K.J. Jiang; F.Y. Zhang; M. Zhao; L.B. Ma; W. Song; K.J. Jiang; F.Y. Zhang; M. Zhao; L.B. Ma
Antimicrobial activity analysis; Codon optimization; Heat resistance; Hemolytic activity; Porcine β-defensin-2; Recombinant expression

Porcine β-defensin-2 (pBD2) is a cationic antimicrobial peptide that has therapeutic potential. The amount of pBD2 in nature is limited, and the expression of pBD2 in Escherichia coli is low, probably because a different gene codon is used by prokaryotic organisms to that used by eukaryotes. Codon preference optimization is one of the ways to increase heterologous expression of ... more

C.Y. Gao; T.T. Xu; Q.J. Zhao; C.L. Li