Research Article

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12/02/2013
Apoptosis; Heat stress; Hsp90α; Myocardial cells; Rat

To understand the mechanism underlying the sudden animal death caused by acute heart failure during heat stress, the relationships among the heat-induced pathological changes and apoptosis and the variations in the levels of protective Hsp90α and its mRNA in the heat-stressed primary myocardial cells of neonatal rats in vitro were studied by cytopathological observation, ... more

A. Islam; Y.J. Lv; A. Abdelnasir; B. Rehana; Z.J. Liu; M. Zhang; S. Tang; Y.F. Cheng; H.B. Chen; J. Hartung; E.D. Bao
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Apoptosis; Hepatocellular carcinoma; Methylation regulation; MicroRNA-122

The regulation mechanism and significance of microRNA-122 (miRNA-122) expression are unclear. The aim of this study was to investigate the effects of DNA methylation on liver-specific miRNA-122 expression, cell proliferation, and apoptosis in hepatocellular carcinoma. Methylation of the miRNA-122 promoter region was detected through methylation sequencing. The level of miRNA-122 ... more

T.J. Xing; H.T. Xu; W.Q. Yu; D.F. Jiang
04/02/2013
Apoptosis; B-16; Diterpene; HeLa; MTT

Cancer has become the leading cause of death in developing countries due to increased life expectancy of the population and changes in lifestyle. Studies on active principles of plant have motivated researchers to develop new antitumor agents that are specific and effective for treatment of neoplasms. Kaurane diterpenes are considered important compounds in the development of new and ... more

V.C. Fernandes; S.I.V. Pereira; J. Coppede; J.S. Martins; W.F. Rizo; R.O. Beleboni; M. Marins; P.S. Pereira; A.M.S. Pereira; A.L. Fachin
04/02/2013
Apoptosis; Bladder cancer; Gene expression; RT-PCR

We examined the expression of anti-apoptotic genes (XIAP and Bcl-2) and apoptotic genes (cytochrome c, caspase-9, Apaf-1) in tissue samples of patients with superficial bladder cancer. Thirty-two bladder cancer tissue samples (8 papillary urothelial neoplasm of low malignant potential, 10 low-grade, and 14 high-grade) and 8 normal bladder ... more

D.F. Barione; F.S.N. Lizarte; P.C. Novais; C.A.M. de Carvalho; F.C.B. Valeri; F.M. Peria; H.F. de Oliveira; D.L. Zanette; W.A. Silva; A.J. Cologna; R.B. Reis; S. Tucci; A.C.P. Martins; D.P.C. Tirapelli; L.F. Tirapelli
08/24/2012
14-3-3 Proteins; Antioxidant; Apoptosis; Glioma; Heat shock protein 27 (Hsp27); Viscum album

Extracts of mistletoe (Viscum album) are intensively used in complementary medicine, but their mechanisms are not fully understood in most cases, and the effects on metabolism have not been investigated in detail. However, some biologically active natural products are well known to provoke unexpected cellular responses. They reduce overexpression of heat shock proteins (Hsps) in ... more

E.Ö. Uçar; N. Arda; A. Aitken
07/19/2012
Apoptosis; Fusion protein; hA20; High glucose; HUVECs; Protein transduction domain

We constructed a plasmid containing a protein transduction domain (PTD) and a human A20 (hA20) gene fragment; the fusion protein was obtained by highly expressing this plasmid in the yeast Pichia pastoris GS115. The plasmid was obtained by adding 9xArg and EcoRІ recognition sites to the end of the primer, and 6xHis-Tag and NotІ recognition sites to its end. ... more

C.L. Hou; Q. Huang; Y. Wei; W. Zhang; J.H. Mi; D.J. Ying; Z.H. Zhou
12/18/2014
Apoptosis; Atorvastatin; Cerebral ischemia and reperfusion; Cytochrome C; Mitochondrial permeability transition pore

The aim of this study was to investigate the influence of atorvastatin on the opening of the mitochondrial permeability transition pore (MPTP) and the expression of cytochrome C (Cyt C) in Sprague-Dawley rats with cerebral ischemia-reperfusion (I/R). The rat model of cerebral artery ischemia was established by the suture-occluded method with ischemia for 2 h and reperfusion for 72 h. ... more

T. Song; J. Liu; X. Tao; J.G. Deng
12/19/2014
Apoptosis; Cytochalasin B; Glioma; mitosis

Cytochalasin B (CB) is known to inhibit a number of cancer types, but its effects on gliomas are unknown. We examined the in vitro effects of CB on the proliferation of human glioma U251 cells, as well as determined its mechanism of action. Cell proliferation was determined using CCK-8. The effect of CB on U251 cell morphology was observed under a transmission electron microscope ... more

Z.G. Tong; N. Liu; H.S. Song; J.Q. Li; J. Jiang; J.Y. Zhu; J.P. Qi
11/27/2014
Apoptosis; Biological behavior; Bladder cancer; Enhancer of zeste homolog 2; siRNA

We investigated the effect of siRNA targeting enhancer of EZH2 on cell proliferation, invasion, migration, and apoptosis of human bladder cancer T24 cells. An siRNA-expressing plasmid targeting the EZH2 gene was transfected into T24 cells. Quantitative polymerase chain reaction and Western blot analysis were used to detect EZH2 expression at the mRNA and protein levels, ... more

H.F. Wang; H. Yang; L.B. Hu; Y.H. Lei; Y. Qin; J. Li; C.W. Bi; J.S. Wang; Q. Huo
10/31/2014
Antigenotoxicity; Apoptosis; Necrosis; Tephrosia cinerea

Tephrosia cinerea L. (Pers.) is a tropical species that exhibits antileishmanial activity in Leishmania amazonensis promastigote cultures and is commonly used to treat infections, inflammations, ulcers, nervous conditions, and diarrhea. However, no studies have investigated its effects on genetic material. Therefore, we evaluated the genotoxic potential, antigenotoxic ... more

A.C.S. Dias; V.R. Moreira; L.P. Almeida; M.I.S. Lima; J.L. Bezerra; M.N.S. Ribeiro; F.R.F. Nascimento; S.R.F. Pereira

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