Research Article

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Here, we analyzed the distribution of H-FABP/(HinfI, MspI, and HaeIII) and ACSL4/RsaI polymorphisms, and the associations of these 4 polymorphic loci with intramuscular fat (IMF) content and backfat thickness (BFT) in Yanan, Jinhua, Duroc, Landrace, Yorkshire, and Duroc x (Landrace x Yorkshire) (DLY) pigs. H-FABP/Hin ... more

J.N. Chen; Y.Z. Jiang; W.M. Cen; S.H. Xing; L. Zhu; G.Q. Tang; M.Z. Li; A.A. Jiang; P.E. Lou; A.X. Wen; Q. Wang; T. He; G.X. Zhu; M. Xie; X.W. Li
05/09/2014
Escherichia coli F18; Pig; Real-time polymerase chain reaction; TAP1 gene

The transporter associated with antigen processing (TAP) transports peptides from the cytosol into the endoplasmic reticulum for subsequent loading onto the major histocompatibility complex (MHC) class I molecules. This study showed the dynamic changes in the TAP1 expression level in newborn to weaning piglets. Tissue expression profiles revealed that the TAP1 gene was ... more

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01/14/2014
Association analysis; Pig; Reproductive trait; RFRP gene; SNP

RFamide-related peptide (RFRP) gene is a crucial gene of the hypothalamo-pituitary-gonadal axis and plays an important role in reproduction regulation. This study aimed to clone and characterize the pig RFRP gene. We obtained a 645-bp cDNA of pig RFRP gene comprising a 546-bp open reading frame, which encoded a peptide of 188 amino acids. The pig RFRP coding ... more

M.X. Fang; Y.S. Huang; J. Ye; W. Zhang; Y. Li; Q.H. Nie
02/28/2014
Expression patterns; Pig; Promoter activity; UGP2

The function of the UDP-glucose pyrophosphorylase 2 gene (UGP2) in pig is not clear. In the present study, we used RNA isolated from Large White pigs and Chinese indigenous MeiShan pigs to examine the temporal coordination of changes in gene expression within muscle tissues. We cloned both the complete genomic DNA sequence and 2077-bp 5ꞌ-flanking sequence of porcine ... more

J.Y. Yu; S.M. Shao; K. Chen; M.G. Lei; Y.Z. Xiong
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Adipose tissue; Conjugated linoleic acid; Gene expression; MicroRNA; Pig

In this study, we evaluated the effect and possible mech­anism of action of dietary conjugated linoleic acid (CLA) on pig body fat deposition. Landrace piglets (N = 48) were randomly divided into three groups, which were fed diets containing 0% (control), 1%, or 2% CLA. Dorsal and abdominal subcutaneous adipose tissues were col­lected, and real-time polymerase chain reaction (PCR) was ... more

R.L. Qi; Y. Chen; J.X. Huang; F.Y. Yang
06/09/2015
BP1 gene; Combined genotype; Immune indices; Pig; Single nucleotide polymorphisms

The bactericidal/permeability-increasing protein (BPI) gene has been identified as a candidate gene for disease-resistance breeding. We evaluated whether polymorphisms in exons 4 and 10 of the BPI gene are associated with immune indices [interleukin-2 (IL-2), IL-4, IL-6, interferon-b (IFN-b), IL-10, and IL-12]. In this study, we identified one mutation (C522T) in the ... more

Z.C. Wu; Y. Liu; Q.H. Zhao; S.P. Zhu; Y.J. Huo; G.Q. Zhu; S.L. Wu; W.B. Bao
03/31/2015
Gene function; PGM1; Pig; Promoter region; Skeletal muscle

The phosphoglucomutase 1 (PGM1) gene was differentially expressed in tissues of Chinese Meishan and Large White pigs. In this study, the promoter region, expression profile, and genetic mutations of the gene were determined. Expression of a 5'-deletion in both C2C12 and PK-15 cells showed that a negative regulatory element was at -1871 to +185 bp and a positive regulatory ... more

J.Y. Yu; S.M. Shao; Y.Z. Xiong
03/06/2015
Gene expression; Growth stages; Mitochondrial DNA copy number; Pig

Mitochondrial DNA (mtDNA) content is dependent on the energy requirements of tissues. To date, no comprehensive study has been conducted to examine mtDNA copy number variations in pigs. In the current study, quantitative polymerase chain reaction was performed to quantify the mtDNA copy number in 15 pig tissue types at 5 growth stages from embryo to adult. Observable differences in mtDNA ... more

Y.M. Xie; L. Jin; X.J. Chen; M.N. He; Y. Wang; R. Liu; M.Z. Li; X.W. Li
02/13/2015
Exclusion power; Microsatellites; Parentage analysis; Pig; Single nucleotide polymorphism

Parentage analysis and individual identification are recent, promising methods that have been applied to evolutionary and ecological studies, as well as conservation management. Parental exclusion relying on polymorphic microsatellites has been used worldwide in parentage determination, while the low mutation rate and genotyping error rate of single nucleotide polymorphisms (SNPs) make ... more

G.C. Yu; Q.Z. Tang; K.R. Long; T.D. Che; M.Z. Li; S.R. Shuai
02/13/2015
Developmental expression; Glucose transporter; Longissimus dorsi muscle; Pig; Quantitative real-time PCR

Glucose transporter proteins 2 and 4 (GLUT2 and GLUT4) play important roles in glucose transport and energy metabolism. Changes in the levels of GLUT2 and GLUT4 mRNA were measured in longissimus dorsi muscle from the lean Yorkshire and fat Tibetan pig breeds at six different time points (1, 2, 3, 4, 5, and 6 months) with quantitative real-time polymerase chain reactions. ... more

Y. Liang; X.M. Yang; Y.R. Gu; X. Tao; Z.Z. Zhong; J.J. Gong; X.H. Chen; X.B. Lv

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