Research Article

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Apoptosis; Curcumin; Invasion; Melanoma; Migration; Proliferation

Malignant melanoma is a melanocytic tumor with a high potential of invasion and metastasis. Curcumin is extracted from Curcuma longa L.; curcumin has anti-tumor efficacy in multiple systemic malignancies. Here, we investigated the effect of curcumin on A375 human melanoma cells. A375 cells were cultivated, passaged, and treated with different concentrations of curcumin. We ... more

Y.P. Zhang; Y.Q. Li; Y.T. Lv; J.M. Wang
Apoptosis; Apoptotic pathway; Bombyx batryticatus; Cytotoxicity; Hela cells

Anticancer activity of Bombyx batryticatus ethanol extract (BBE) against HeLa cells was studied using cell viability, DNA fragmentation, real-time polymerase chain reaction, and Western blot analyses. The BBE inhibited the growth and induced apoptosis of HeLa cells. The MTT assay indicated that the BBE induced cytotoxicity in HeLa cells in a time- and concentration-dependent ... more

W.P. Wu; J. Cao; J.Y. Wu; H. Chen; D. Wang
Apoptosis; BPH-1 cells; Cell cycle; Croton membranaceus; Proliferation

Croton membranaceus aqueous root extract (CMARE) is among the widely used phytotherapeutics in Ghana for the management of benign prostatic hyperplasia (BPH) and prostate cancer. However, the mechanism of action of CMARE remains to be elucidated. This study aimed to establish whether apoptosis is involved in the antiproliferative effect of CMARE on human BPH-1 cells. We ... more

D.K. Afriyie; G.A. Asare; K. Bugyei; J. Lin; J. Peng; Z. Hong
Apoptosis; Drosophila melanogaster; Eye development; UAS/GAL4 ectopic expression system

The UAS/GAL4 ectopic expression system is widely used in Drosophila melanogaster for the overexpression of transgenes. This system operates under the assumption that the yeast transcription factor, GAL4, is inactive in D. melanogaster. Thus, GAL4 can be expressed under the control of D. melanogaster-specific promoters with little effect upon the organism. We ... more

J.M. Kramer; B.E. Staveley
Activated caspase-3; Apoptosis; Gastric cancer; Gastric dyspepsia; Helicobacter pylori; TUNEL

Apoptosis has an essential function in maintaining the integrity of the gastrointestinal mucosa. Its deregulation is associated with the occurrence of lesions such as in atrophic gastritis, peptic ulcers, intestinal metaplasia, and stomach tumorigenesis. Thus, the aim of the present study was to investigate the frequency of apoptotic cells (apoptotic index, AI) by using two different ... more

A.C. Targa; A.C.G. César; P.M. Cury; A.E. Silva
Apoptosis; Brachiaria hybrid; Chromatin fragmentation; Male sterility; Meiosis; Programmed cell death

Morphological changes have been investigated during plant programmed cell death (PCD) in the last few years due to the new interest in a possible apoptotic-like phenomenon existing in plants. Although PCD has been reported in several tissues and specialized cells in plants, there have been few reports of its occurrence during microsporogenesis. The present study reports a typical ... more

V.A. Fuzinatto; M.S. Pagliarini; C.B. Valle
Apoptosis; Cell cycle control; Childhood brain tumor; Single nucleotide polymorphism

We conducted a hospital-based case-control study in Korea to investigate whether apoptosis- and cell cycle control-related genes are associated with childhood brain tumor. Incident brain tumor cases (N = 70) and non-cancer controls (N = 140), frequency-matched by age and gender, were selected from 3 teaching hospitals in Seoul between 2003 and 2006. Tag single nucleotide polymorphisms ( ... more

S. Jeon; S. Han; K. Lee; J. Choi; S.K. Park; A.K. Park; H.S. Ahn; H.Y. Shin; H.J. Kang; H.H. Koo; J.J. Seo; J.E. Choi; H. Kim; Y. Ahn; D. Kang
Apoptosis; Gene and protein expression; MGC; shRNA; siRNA; VEGF

Vascular endothelial growth factor (VEGF) has been found responsible for the induction of proliferation and differentiation in granulosa cells. We constructed four short hairpin RNA (shRNA) expression plasmids targeting the mouse VEGFA gene, and examined their effect on VEGF expression in mouse granulosa cells (MGC) in vitro. Four different shRNA oligonucleotides targeting the ... more

N. Ally; X.L. Zou; B.C. Jiang; L. Qin; L. Zhai; P. Xiao; H.L. Liu
Apoptosis; BMPRIB; Porcine granulosa cells; siRNA; Steroidogenesis

Bone morphogenetic proteins (BMPs) are the key factors in maintaining cell growth and differentiation in ovaries. BMPs initiate signaling by assembling BMP receptors and activating Smads, which in turn alter the expression of target genes. However, little is known about the effect of the deletion of the Bone morphogenetic protein receptor type IB (BMPRIB) on porcine granulosa cell (GCs ... more

Y.Y. Zhao; X.X. Li; W. Wang; X. Chen; P. Yu; J.J. Wang; Y.X. Xu
Apoptosis; Apoptotic protein; Ischemia-reperfusion; NGAL; Tubular epithelial cells

This study aimed to determine the protective effect and mechanism of neutrophil gelatinase-associated lipocalin (NGAL) in rat kidney on ischemia/reperfusion injury (I/R). The rat I/R model was set up by cutting one kidney and clamping the contralateral renal pedicle for 45 min. Male SD rats were randomly divided into sham-operation, I/R and NGAL groups. Hematoxylin-eosin staining was ... more

X.J. Zang; S.X. An; Z. Feng; Y.P. Xia; Y. Song; Q. Yu