Research Article

Related GMR Articles

Apoptosis; DNA damage; genomic instability; Inflammation; Isocyanates; Lung cancer

Lungs comprise the primary organ exposed to environmental toxic chemicals, resulting in diverse respiratory ailments and other disorders, including carcinogenesis. Carcinogenesis is a multi-stage phenomenon, which involves a series of genetic alterations that begin with genomic instability provoked by certain factors such as inflammation and DNA damage and end with the development of cancer. ... more

P.K. Mishra; A. Bhargava; G.V. Raghuram; S. Gupta; S. Tiwari; R. Upadhyaya; S.K. Jain; K.K. Maudar
Apoptosis; Arg72Pro; Karyorrhexis; Micronuclei; rs56275308; TP53

Polymorphisms in the TP53 gene codon 72 (Arg72Pro) influence apoptosis induction and DNA damage repair. We evaluated how variants of protein p53 (p53Arg and p53Pro) affect cell death and DNA damage repair by analyzing the frequencies of karyorrhexis and micronuclei. There were significant differences in the frequency of karyorrhexis between the three p53 genotypes (Arg/Arg, Arg/ ... more

L. Pereira; M.R.S. Carvalho; C.G. Fonseca; S.S.S. Lima; E.M.M. Cerqueira; W. Jorge; M.C.L. Castro
Apoptosis; CDV; Fas receptor; RT-PCR; Vero cells

We evaluated the expression of the Fas receptor gene in Vero cells infected with the Lederle vaccine strain of canine distemper virus using RT-PCR. Vero cells were plated, and after being grown for 24 h in MEM with 5% FBS, 80-90% confluent monolayer cultures were infected with the virus. The cells were harvested at 3, 6, 9, and 15 h post-infection. Uninfected Vero cells were used as a ... more

H.L. Del Puerto; A.S. Martins; G.F. Braz; F. Alves; M.B. Heinemann; D.S. Rajão; F.C. Araújo; S.F. Martins; D.R. Nascimento; R.C. Leite; A.C. Vasconcelos
Angiotensin-II; Anti-aging; Apoptosis; IGF-1; klotho gene; PTH

Inactivation of the klotho gene in mice causes serious systemic disorders, resembling human aging. However, at the molecular level, its action mechanisms are not well understood. The stimulatory or inhibitory effects of cis- and trans-regulatory factors on the klotho gene expression are also still unclear. We studied the effects of intra- and extracellular factors on ... more

K. Turan; P. Ata
Apoptosis; HSIL; LSIL; Micronucleus

We evaluated micronucleus and apoptosis occurrence among women with normal smears and women with different kinds of cervical abnormalities, i.e., inflammatory processes and low- and high-grade squamous intraepithelial lesions (N = 12, N = 10 and N = 27, respectively). The sample included 59 women who were seen at a public medical service for cervical cancer prevention in Feira de Santana, ... more

G.M.A. Aires; J.R.C. Meireles; P.C. Oliveira; J.L. Oliveira; E.L. Araújo; B.C. Pires; E.S.A. Cruz; N.F. Jesus; C.A.B. Pereira; E.M.M. Cerqueira
A20 gene; Apoptosis; High glucose; HUVECs; Monocyte homing

Diabetes mellitus causes vascular lesions and may ultimately lead to atherosclerosis. One of the earliest steps in the development of atherosclerotic lesions is the adhesion of monocytes to endothelial cells of the vessel wall. It is currently unknown whether zinc finger protein A20 is able to protect endothelial cells from injury caused by high levels of glucose and monocyte homing. In ... more

C.L. Hou; W. Zhang; Y. Wei; J.H. Mi; L. Li; Z.H. Zhou; W. Zeng; D.J. Ying
Apoptosis; Heat stress; Hsp90α; Myocardial cells; Rat

To understand the mechanism underlying the sudden animal death caused by acute heart failure during heat stress, the relationships among the heat-induced pathological changes and apoptosis and the variations in the levels of protective Hsp90α and its mRNA in the heat-stressed primary myocardial cells of neonatal rats in vitro were studied by cytopathological observation, ... more

A. Islam; Y.J. Lv; A. Abdelnasir; B. Rehana; Z.J. Liu; M. Zhang; S. Tang; Y.F. Cheng; H.B. Chen; J. Hartung; E.D. Bao
Apoptosis; Hepatocellular carcinoma; Methylation regulation; MicroRNA-122

The regulation mechanism and significance of microRNA-122 (miRNA-122) expression are unclear. The aim of this study was to investigate the effects of DNA methylation on liver-specific miRNA-122 expression, cell proliferation, and apoptosis in hepatocellular carcinoma. Methylation of the miRNA-122 promoter region was detected through methylation sequencing. The level of miRNA-122 ... more

T.J. Xing; H.T. Xu; W.Q. Yu; D.F. Jiang
Apoptosis; B-16; Diterpene; HeLa; MTT

Cancer has become the leading cause of death in developing countries due to increased life expectancy of the population and changes in lifestyle. Studies on active principles of plant have motivated researchers to develop new antitumor agents that are specific and effective for treatment of neoplasms. Kaurane diterpenes are considered important compounds in the development of new and ... more

V.C. Fernandes; S.I.V. Pereira; J. Coppede; J.S. Martins; W.F. Rizo; R.O. Beleboni; M. Marins; P.S. Pereira; A.M.S. Pereira; A.L. Fachin
Apoptosis; Invasion; miR-181b; PC-3; Proliferation; Prostate cancer

We examined microRNA-181b (miRNA) expression in prostate cancer tissues and its effect on the prostate cancer cell line PC-3. Tissues from 27 cases of prostate cancer and 30 samples of normal human prostate were collected by surgical removal. Total miRNA was extracted, and the relative expression of miR-181b was quantified using RT-PCR. miR-181b ASO was transfected into prostate cancer PC ... more

L. He; H. Yao; L.H. Fan; L. Liu; S. Qiu; X. Li; J.P. Gao; C.Q. Hao