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Related GMR Articles

06/20/2007
Canidae; Chrysocyon brachyurus; Cross-amplification; Domestic dog; Microsatellite; Population genetics

The maned wolf (Chrysocyon brachyurus) is the largest South American canid. Habitat loss and fragmentation, due to agricultural expansion and predatory hunting, are the main threats to this species. It is included in the official list of threatened wildlife species in Brazil, and is also protected by IUCN and CITES. Highly variable genetic markers such as microsatellites have ... more

D.C. Salim; A.A. Akimoto; C.B. Carvalho; S.F. Oliveira; C.K. Grisolia; J.R. Moreira; M.N. Klautau-Guimarães
12/19/2011
Cross-amplification; Lycoris radiata; Microsatellite; Molecular marker

Lycoris radiata is a perennial herb that has been used in traditional Chinese medicine for a long time and has two main medicinal components in its bulb, lycorine and galanthamine. However, the original microsatellite loci have not been developed for any species of Lycoris. Total genomic DNA was extracted from fresh bulbs using a modified CTAB protocol. We isolated 10 ... more

S.Q. Xuan; J.Y. Zheng; H. Wang; P. Gao; S.B. Zhou; K. Liu; G.P. Zhu
07/12/2011
Cross-amplification; Genetic variability; SSR; Transferability

Leporinus friderici, native to the Amazon Basin and popularly known as “piau-três-pintas”, has great ecological and economic importance; it is widely fished and consumed throughout much of tropical South America. Knowledge of the genetic diversity of this native species is important to support management and conservation programs. We evaluated microsatellite loci amplification, ... more

A.M. Olivatti; T.A. Boni; N.J. Silva-Júnior; L.V. Resende; F.O. Gouveia; M.P.C. Telles
12/06/2013
Cross-amplification; Microsatellite markers; Nyctereutes procyonoides procyonoides

Chinese raccoon dog (Nyctereutes procyonoides procyonoides) is one of the most important fur-bearing animal species. Information about the genetic background of farmed Chinese raccoon dogs is limited. In this study, 17 polymorphic microsatellite markers were isolated and identified from an (AC)n-microsatellite-enriched library of Chinese raccoon dogs. The number of ... more

S.Q. Yan; Y.M. Li; C.Y. Bai; X.M. Ding; W.J. Li; J.N. Hou; Z.H. Zhao; J.H. Sun
02/08/2013
Codominant markers; Cross-amplification; Microsatellite isolation; Tree genetics

Chrysophyllum gonocarpum is a tropical tree species that is very important in the recovery of heterogeneous forests and of degraded areas of permanent preservation. We identified microsatellite loci for C. gonocarpum to assess the genetic variability and the patterns of the population structure of the species. We isolated 8 microsatellite primers by using CT- and GT- ... more

L.A. Rodrigues; E.A. Ruas; N.C. Signori; B.F. Costa; D.C.L. Farias-Missão; B.G. Vieira; A.R.O. Conson; C.L. Chaves; J. Delfini; C.F. Ruas; P.M. Ruas
12/06/2012
Anopheles albitarsis; Cross-amplification; Malaria vectors; Microsatellite-enriched library; Species complex; SSR

The Anopheles albitasis complex includes 6 species, and 3 are considered as malaria vectors in Brazil. Twenty-five polymorphic microsatellite DNA loci were isolated and characterized in 24-36 individuals from the neighborhood of Puraquequara, Manaus, Amazonas State, Brazil. The number of estimated alleles ranged from 2 to 10, the observed heterozygosity ranged from 0.182 to 0.897 ... more

G.M. Guimarães-Marques; J.S. Batista; H.M. Guimarães; M.V. Naice-Daou; M.P. Lima; K.M. Formiga; J.M.M. Santos; C.A.P. Lima; M.S. Rafael
08/13/2012
Cross-amplification; Fabaceae; Genetic diversity; Microsatellite libraries; SSR; Tree

Parapiptadenia rigida, locally known as angico, is a tropical tree common in the semideciduous Brazilian forest. Its wood is naturally resistant to insect attack and is useful for construction. Extracts from the tree have medicinal properties. We characterized nine microsatellite loci for P. rigida. Thirty-five alleles were detected in a sample of 45 individuals from 3 ... more

B.F. Costa; L.A. Rodrigues; E.A. Ruas; L.B. Souza; C.F. Ruas; B.G. Vieira; A.R.O. Conson; P.M. Ruas
12/04/2014
Cross-amplification; Fluorescence in situ hybridization; Forage; Microsatellite

Aeschynomene falcata is an important forage species; however, because of low seed production, it is underutilized as forage species. Aeschynomene is a polyphyletic genus with a challenging taxonomic position. Two subgenera have been proposed, and it is suggested that Aeschynomene can be split in 2 genera. Thus, new markers, such as microsatellite sequences, are ... more

C.A. Polido; C.C. Mantello; A.P. Moraes; A.P. Souza; E.R. Forni-Martins
07/07/2014
Atlantic rainforest; Cross-amplification; Ocotea catharinensis; Ocotea odorifera; Ocotea porosa; Single sequence repeat

The Atlantic rainforest species Ocotea catharinensis, Ocotea odorifera, and Ocotea porosa have been extensively harvested in the past for timber and oil extraction and are currently listed as threatened due to overexploitation. To investigate the genetic diversity and population structure of these species, we developed 8 polymorphic microsatellite ... more

E.M. Martins; G. Martinelli; M.P. Arbetman; R.W. Lamont; J.L. Simões-Araújo; D. Powell; M. Ciampi-Guillardi; C. Baldauf; A. Quinet; P. Galisa; A. Shapcott
01/22/2014
Cross-amplification; Microsatellite markers; Population genetics; Siniperca chuatsi

The mandarin fish is a popular fresh water food fish in China. Fifty-three polymorphic microsatellite markers were isolated through construction of an enriched library of genomic DNA of Siniperca chuatsi (Percichthyidae). We found 2 to 7 alleles per locus. The observed and expected heterozygosity values varied from 0.059 to 1.000 and from 0.305 to 0.818, respectively. The ... more

C.X. Tian; X.F. Liang; M. Yang; Y.Q. Dou; H.Z. Zheng; L. Cao; Y.C. Yuan; C. Zhao

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