Research Article

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09/05/2014
DNA sequencing; GH gene; Growth traits; Polymerase chain reaction single-strand conformation polymorphism; Polymorphism; Sheep

The growth hormone gene plays an important role in the physiological function of an organism. The current study aimed to investigate the correlation between polymorphisms in the 5ꞌ regulatory region, exon 4, and 3ꞌ untranslated region (UTR) of the sheep GH gene and sheep growth traits. The DNA from 510 adult sheep was analyzed by DNA sequencing and polymerase chain reaction single ... more

J.L. Jia; L.P. Zhang; J.P. Wu; Z.J. Ha; W.W. Li
12/21/2005
Breast cancer; Polymorphisms; RAD51-G135C; XPD-Lys751Gln; XRCC1-Arg399Gln; XRCC3-Thr241Met

Several studies have reported that the genes involved in DNA repair and in the maintenance of genome integrity play a crucial role in protecting against mutations that lead to cancer. Epidemiologic evidence has shown that the inheritance of genetic variants at one or more loci results in a reduced DNA repair capacity and in an increased risk of cancer. Polymorphisms have been ... more

R.Mucha Dufloth; S. Costa; F. Schmitt; L.Carlos Zeferino
04/13/2005
DNA; Minisequencing; Multiplex; Polymorphisms; Single nucleotide polymorphisms

Several technologically sophisticated high-throughput techniques have been recently developed for the study of human single nucleotide polymorphisms and the diagnosis of point mutations in human diseases. However, there is also a need for simple and inexpensive techniques suitable for clinical services and small research laboratories. Minisequencing meets the latter requirements. It is ... more

C.M.B. Carvalho; S.D.J. Pena
12/01/2009
Childhood acute lymphoblastic leukemia; Haplotypes; Mexicans; Polymorphisms; XRCC1

We examined the influence of the Arg194Trp, Arg280His, and Arg399Gln polymorphisms of XRCC1 (X-ray repair cross-complementing group 1) on the development of childhood acute lymphoblastic leukemia (ALL) in 120 ALL patients and 120 controls in Mexico. All of them were genotyped for these polymorphisms, using polymerase chain reaction. No significant differences in allele and ... more

J.P. Meza-Espinoza; V. Peralta-Leal; M. Gutierrez-Angulo; N. Macias-Gomez; M.L. Ayala-Madrigal; P. Barros-Nuñez; J. Duran-Gonzalez; E. Leal-Ugarte
07/01/2008
Breast cancer; estrogen; Hereditary disease; Polymorphisms

The association of tumor differentiation and estrogen receptor expression with the prognosis of breast cancer has been well established. Nevertheless, little is yet reported about the association of morphological characteristics of the tumor, estrogen receptor status and polymorphisms in low penetrance genes. The aim of the present study was to investigate a possible association between DNA ... more

R.M. Dufloth; A. Arruda; J.K.R. Heinrich; F. Schmitt; L.C. Zeferino
01/08/2008
Allele specific polymerase chain reaction; Mexican populations; Orosomucoid; Polymorphisms; Sequencing; Teenek Amerindians

The human orosomucoid 1 gene (ORM1) codes an alpha-1-acid glycoprotein that has been classified as an acute-phase reactive protein, and a major drug-binding serum component, as well as an immunomodulatory protein with genetic polymorphisms. Evaluation of ORM variation through isoelectric focusing and immunobloting has revealed a world-wide distribution of the ORM1 F and ORM1 S alleles. We ... more

L. García-Ortiz; G. Vargas-Alarcón; J.M. Fragoso; J. Granados; M. Noriega; N. Pérez; H. Reyes; J.C. Zenteno-Ruiz; E. Martínez-Cordero
12/19/2011
hOGG1; Oxidative stress; Polymorphisms; XPD

Knee replacement surgery is an ischemia/reperfusion model, as it uses tourniquet applied to the knee area to stop the blood flow during the operation. Fifty patients that were undergoing elective arthroscopic knee surgery were included in our study. Human 8-oxoguanine glycosylase 1 (hOGG1) is an enzyme to repair specific DNA lesions and a good marker of hydroxyl radical damage to DNA. XPD ... more

O. İsbir; U. Gormus; H.A. Ergen; B. Cakmakoglu; Ö.T. Kahraman; N. Baykan
11/04/2011
Body measurement traits; Cattle; MyoG; Polymorphisms

Using PCR-SSCP and DNA sequencing technology, we examined the association of single nucleotide polymorphisms (SNPs) in the bovine MyoG gene with body measurement traits in 779 individuals of six native Chinese cattle breeds, namely Luxi, Luxi × Simmental crossbred, Nanyang, Xia’nan, Jiaxian red, and Qinchuan. A novel SNP, T314C, was detected. Allelic frequencies of MyoG- ... more

M. Xue; L.S. Zan; L. Gao; H.B. Wang
11/04/2011
Albuminuria; Essential hypertension; Lectin-like oxidized low-density lipoprotein receptor 1; Polymorphisms

Albuminuria is an independent predictor of renal and cardiovascular complications in hypertensive subjects. We previously showed that lectin-like oxidized low-density lipoprotein receptor 1 (OLR-1) polymorphisms at G501C are associated with susceptibility to essential hypertension and serum C-reactive protein levels. We have now investigated a possible association between OLR-1 polymorphisms ... more

X.W. Hou; Z.H. Hu; Y. Cui; W. Gao; S.P. Sun; N.F. Wang
10/25/2011
Congenital heart defect; Folic acid; Homocysteine; Methionine synthase reductase; Polymorphisms

Congenital heart defects (CHDs) are the most common birth defects; genes involved in homocysteine/folate metabolism may play important roles in CHDs. Methionine synthase reductase (MTRR) is one of the key regulatory enzymes involved in the metabolic pathway of homocysteine. We investigated whether two polymorphisms (A66G and C524T) of the MTRR gene are associated with ... more

W. Zeng; L. Liu; Y. Tong; H.M. Liu; L. Dai; M. Mao

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