Research Article

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01/24/2013
Cultivar identification; Ginkgo biloba; RAPD markers

Cultivar identification is a key step to avoid the formation of homonyms and synonyms of Ginkgo biloba. In this study, a new approach based on combinational utilization of polymorphic bands produced from 6 different random amplified polymorphic DNA (RAPD) primers was developed for identifying 42 Ginkgo cultivars, and a manual cultivar identification diagram that ... more

G.P. Li; C.Q. Zhang; F.L. Cao
01/07/2013
Cultivar identification; Molecular markers; Pennisetum glaucum; Seed piracy; SSR

The increasing number of protected and registered cultivars and problems involving seed commercialization make distinction and identification of cultivars imperative. Millet (Pennisetum glaucum), a crop species with protected cultivars in Brazil, has been the target of seed piracy. Thus, with the objective of identifying different lots with regard to origin, we characterized six ... more

R.P.Mendonça Neto; E.V.R. Von Pinho; B.L. Carvalho; G.S. Pereira
08/06/2012
Cultivar identification; Molecular markers; RAPD; Sweet orange

We optimized RAPD techniques by increasing the length of RAPD primers and performing a strict screening of PCR annealing temperature to distinguish 60 sweet orange cultivars from the Research Institute of Pomology at the Chinese Academy of Agricultural Sciences. A new approach called cultivar identification diagram (CID) was used to improve the efficiency of RAPD markers for cultivar ... more

X. Sun; Q. Mu; D. Jiang; C. Wang; X.C. Wang; J.G. Fang
05/07/2012
Cultivar identification; Molecular markers; Peach; RAPD

DNA markers have useful applications in cultivar identification. A novel analysis approach called cultivar identification diagram (CID) was developed using DNA markers in the separation of plant individuals. This new strategy is less time- and cost-consuming, has reliable results, and was constructed for fingerprinting. Ten 11-mer primers were used to amplify the genotypes; all 95 peach ... more

M.L. Yu; W.Y. Wang; R.J. Ma; Z.J. Shen; J.G. Fang
01/08/2014
Cultivar identification; Molecular markers; Ornamental peach; RAPD

One of the most important uses of DNA markers is cultivar identification. However, no DNA fingerprint analysis strategy is available for making DNA markers helpful in practical plant cultivar identification, especially for the identification of a large number of cultivars. We developed a manual cultivar identification diagram strategy for efficient identification of plant cultivars, from ... more

J. Han; W.Y. Wang; X.P. Leng; L. Guo; M.L. Yu; W.B. Jiang; R.J. Ma
06/01/2015
Cultivar identification; Genetic diversity; Genetic similarity; ISSR; Olea europaea L.; SSR

The olive tree is an iconic tree of the Mediterranean, and is used extensively to produce high-quality olive oil. Although the China olive industry has just begun to be valued, there were also existed mislabeling and synonyms in introduced cultivars. The aim of this study was to analyze genetic similarities among olive cultivars in China using SSR and ISSR techniques. Thirty-two samples ... more

M.M. Zhan; Z.Z. Cheng; G.C. Su; A.Y. Wang; H.P. Chen; Z.S. Yang; Z. Shan; Q.M. Huang
03/06/2015
Cultivar identification; Cultivar identification diagram (CID); Genetic diversity analysis; Random amplified polymorphic DNA; Tomato

Cultivar identification diagrams (CIDs) provide a rapid and efficient approach for identifying cultivars based on random amplified polymorphic DNA (RAPD) markers. In this paper, 64 tomato cultivars were identified using a CID. Using RAPD profiles, clustering analysis was performed to analyze genetic diversity. The results showed that 8 RAPD primers could completely separate the 64 ... more

X. Cao; Z. Wu; R. Zhou; F.L. Jiang; Z.E. Yang
12/04/2013
Genetic relationship; Korean Pacific abalone; Molecular marker; Random amplified polymorphic DNA (RAPD); URP primer

Random amplified polymorphic DNA (RAPD) with universal rice primers (URP) was used to identify species and to determine phylogenetic relationships for the 6 economically important Korean Pacific abalone species: Haliotis discus hannai, H. discus discus, H. madaka, H. gigantea, H. diversicolor supertexta, and ... more

H.S. An; J.Y. Park; J.I. Myeong; C.M. An
09/03/2013
Genetic relationship; Lolium; Polymorphism; RAPD markers; Taxonomy

The genus Lolium is one of the most important groupings of temperate forage grasses, including about eight recognized species that are native to some temperate and subtropical regions of the northern hemisphere. We examined genetic relationships among 18 accessions representing all Lolium species using RAPD markers. Among 50 random primers that we screened, 13 gave ... more

X. Ma; X.Y. Gu; T.T. Chen; S.Y. Chen; L.K. Huang; X.Q. Zhang
08/31/2012
Curcuma alismatifolia; Genetic relationship; ISSR-PCR; Molecular identification

The genus Curcuma is a member of the ginger family (Zingiberaceae) that has recently become popular for use as flowering pot plants, both indoors and as patio and landscape plants. We used PCR-based molecular markers (ISSRs) to assess genetic variation and relationships between five varieties of curcuma (Curcuma alismatifolia) cultivated in Malaysia. Sixteen ISSR primers ... more

S. Taheri; T.L. Abdullah; N.A.P. Abdullah; Z. Ahmad

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