Research Article

Related GMR Articles

09/27/2013
DNA barcoding; Fallopia multiflora (Thunb.) Harald.; ITS2; matK; psbA-trnH; rbcL

Fallopia multiflora, locally known as Heshouwu, is one of the most important and widely used Chinese medicinal herbs. However, there is still considerable confusion concerning its different provenances. DNA barcoding is a recent aid to taxonomic identification and uses a short standardized DNA region to discriminate plant species. We assessed the applicability of 4 candidate DNA ... more

X.Q. Sun; M.M. Bai; H. Yao; J.L. Guo; M.M. Li; Y.Y. Hang
04/02/2013
8S-ITS2; Brazil; DNA barcoding; Endophytic fungi; Family plants; ITS1-5; Phylogenetic analysis

Brazil has a great diversity of plants, and considering that all plant species studied to date have endophytic microorganisms (bacteria or fungi), the country is a resource in the search for bioactive compounds. Endophytes live within plants without causing damage and may be in dynamic equilibrium with the health of the plant. Endophytic fungi can be identified by sequencing the region ... more

S.A. Rhoden; A. Garcia; J.L. Azevedo; J.A. Pamphile
07/19/2012
DNA barcoding; Identification; Phylogenetics; rbcL; Wild plants

DNA barcoding is currently gaining popularity due to its simplicity and high accuracy as compared to the complexity and subjective biases associated with morphology-based identification of taxa. The standard chloroplast DNA barcode for land plants recommended by the Consortium for the Barcode of Life (CBOL) plant working group needs to be evaluated for a wide range of plant species. We ... more

S.O. Bafeel; I.A. Arif; M.A. Bakir; A.A.Al Homaidan; A.H.Al Farhan; H.A. Khan
09/12/2014
Cytochrome c oxidase I; DNA barcoding; Phasianidae; Phylogeny

A DNA barcode is a short sequence of standardized genomic region that is specific to a species. According to studies of bird species, the 694-bp sequence of the mitochondrial gene encoding cytochrome c oxidase 1 (COI) is extremely useful for species identification and phylogeny. In the present study, we analyzed the COI barcodes of 31 species from 18 genera belonging to the Phasianidae ... more

Z.H. Huang; D.H. Ke
04/16/2014
Cassia; DNA barcoding; ITS2; matK; rbcL; trnH-psbA

DNA barcoding is a desirable tool for medicinal product authentication. DNA barcoding is a method for species identification using short DNA sequences that are conserved within species, but variable between species. Unlike animals, there is no single universal DNA barcode locus for plants. Coding markers, matK and rbcL, and noncoding markers, trnH-psbA ( ... more

N. Purushothaman; S.G. Newmaster; S. Ragupathy; N. Stalin; D. Suresh; D.R. Arunraj; G. Gnanasekaran; S.L. Vassou; D. Narasimhan; M. Parani
02/19/2014
Bats; Biodiversity; DNA barcoding; Hipposideros; Malaysia; species identification

Bats are important flagship species for biodiversity research; however, diversity in Southeast Asia is considerably underestimated in the current checklists and field guides. Incorporation of DNA barcoding into surveys has revealed numerous species-level taxa overlooked by conventional methods. Inclusion of these taxa in inventories provides a more informative record of diversity, but is ... more

J.J. Wilson; K.W. Sing; M.R.A. Halim; R. Ramli; R. Hashim; M. Sofian-Azirun
06/01/2015
Cytochrome c oxidase I; DNA barcoding; Phylogenetic relationship; Polyphyly; Timaliidae

The Timaliidae, a diverse family of oscine passerine birds, has long been a subject of debate regarding its phylogeny. The mitochondrial cytochrome c oxidase subunit I (COI) gene has been used as a powerful marker for identification and phylogenetic studies of animal species. In the present study, we analyzed the COI barcodes of 71 species from 21 genera belonging to the family Timaliidae ... more

Z.H. Huang; D.H. Ke
04/27/2015
DNA barcoding; HRM analysis; rbcL; rpoC; Seagrass; species identification

Biological species are traditionally identified based on their morphological features and the correct identification of species is critical in biological studies. However, some plant types, such as seagrass, are taxonomically problematic and difficult to identify. Furthermore, closely related seagrass species, such as Halophila spp, form a taxonomically unresolved complex. ... more

M. Osathanunkul; P. Madesis; S. Ounjai; C. Suwannapoom; A. Jampeetong
03/13/2015
DNA barcoding; Ixiolirion; species identification; Taxonomy

Ixiolirion is a genus of unresolved taxonomy. DNA barcoding is a technique that allows species identification using standardized DNA sequences. In this study, a total of 23 individuals, representing 2 Chinese Ixiolirion species, were sampled to test the effectiveness of 3 DNA barcodes [internal transcribed spacer (ITS), chloroplast tRNA intron, and megakaryocyte- ... more

Y. Zhao; Y. Li; Y. Liu; Y.F. Yang

Pages