Research Article

Related GMR Articles

Bodhesin; Capra hircus; cDNA; Prokaryotic expression; Recombinant protein; Spermadhesin

Low purification efficiency and incomplete characteriza­tion of male goat (buck) spermadhesins (Bdhs) prompted us to develop an effective system to produce recombinant Bdhs (rBdhs). Bdh-2 cDNA was inserted into a prokaryotic expression plasmid, pTrcHis TOPO. The pTrcHis-Bdh-2 system was constructed to produce a His6 fusion protein in Escherichia coli Top10 cells. Recombinant ... more

J.B. Cajazeiras; L.M. Melo; E.S. Albuquerque; G. Rádis-Baptista; B.S. Cavada; V.J.F. Freitas
FHL2; Fusion protein; GST; Prokaryotic expression; Protein purification

Escherichia coli is the most widely used host for the production of recombinant proteins. However, most eukaryotic proteins are typically obtained as insoluble, misfolded inclusion bodies that need solubilization and refolding. The interactions between human FHL2 protein and many types of proteins, including structural proteins, kinases, and several classes of transcription ... more

H. Yu; Q. Ma; J. Lin; Y.F. Sun; F. Zheng
Gene cloning; MID1IP1; Porcine; Prokaryotic expression

We amplified S14R protein gene cDNA of porcine, cloned it into a prokaryotic expression plasmid, and expressed it in Escherichia coli. A pair of primers was designed based on the cDNA sequence of the porcine S14R gene in GenBank. The target gene fragment from porcine liver tissue was amplified by RT-PCR. Confirmed by auto-sequencing, the target gene fragment was ... more

Y.J. Guo; G.Z. Liu; C.M. Wang; Y.Y. Wang; H.J. Li; K. Zhong; W.F. Lu; Y.L. Wang; G.Y. Yang
Fusion protein; Giant panda; Interleukin-6/interleukin-2; Prokaryotic expression

To construct a fusion cytokine protein with more and stronger bioactivities to enhance the immunity of the cytokine alone, we expressed interleukin (IL)-6/(IL)-2 from giant panda (Ailuropoda melanoleuca) in Escherichia coli as a 59.4-kDa fusion protein. Subsequently, the inclusion bodies were solubilized with 8 M urea and applied onto a Ni-nitrilotriacetic acid column. ... more

Y. Yi; Y.Y. Nian; H.W. Ji; H. Zhang; L. Zhu; Z.W. Xu
Electronic clone; Expression change; Homologue of Serpina3n; Liver regeneration; Prokaryotic expression

A strikingly upregulated expressed sequence tag was screened from regenerating rat liver at 8 h in a 0-4-8-12 h short-interval successive partial hepatectomy model from a previous study. In the present study, a full-length open reading frame (ORF) corresponding to this expressed sequence tag was predicted through electronic cloning and was subsequently cloned from an 8-h rat regenerating ... more

G.P. Wang; X.S. Zhang; Y.H. Li; J.L. Zheng; C.Z. Tang; W.X. Zhang
PpSUT2; Prokaryotic expression; Real-time fluorescent quantitative polymerase chain reaction; Subcellular localization; Sucrose transporter

A 1794-bp cDNA fragment was amplified from mRNA isolated from pear (Pyrus pyrifolia NaKai. Cuiguan) leaves by using primers based on the sequences generated during the analysis of the pear transcriptome. The 597-amino acid sequence encoded by the cDNA was compared with the sequences in GenBank, and it was found to be similar to that of members of the sucrose-proton co-transporter ... more

J. Tang; J. Lin; B.L. Zhang; Z.H. Wang; X.G. Li; Y.H. Chang
Porcine lipoic acid synthase (LIAS); Prokaryotic expression

Lipoic acid synthase, which exists primarily in mitochondria, participates in the biosynthesis of intrinsic lipoic acid. The lipoic acid synthase gene in pig is known as LIAS. To further investigate the biological functions of the protein that is encoded by LIAS, we cloned the open read frame of porcine LIAS (GenBank No. JN797612.1) into the expression vector pET-28α ... more

W.F. Lu; J.J. Cao; Y.J. Guo; K. Zhong; G.M. Zha; L.F. Wang; G.Y. Yang
Hgp44; Human umbilical vein endothelial cell (HUVEC); Interleukins 6 and 8; Porphyromonas gingivalis; Prokaryotic expression; Protein purification

The aim of this study was to clone, express the gene of Hgp44 in adhesin domains of gingipains from Porphyromonas gingivalis and purify the protein. Furthermore, the effect of Hgp44 from P. gingivalis on inducing HUVECs to secrete IL-6 and IL-8 was evaluated. The Hgp44 gene fragment was amplified by polymerase chain reaction, and then inserted into the cloning vector ... more

J. Zhang; H.H. Cui; Y. Wang; Q.X. Zhang; S.L. Deng; Y.D. Chen; H. Chen
Bioinformatic analysis; Cloning; GnRH; Jinghai yellow chicken; Prokaryotic expression

The gonadotropin-releasing hormone (GnRH) plays an important role in the control of reproductive functions. Recent studies have reported the occurrence of GnRH molecular variants in numerous species. In this study, the GnRH1 gene from Jinghai yellow chicken was cloned by reverse transcriptase-polymerase chain reaction and transformed into BL21 (DE3) competent cells. The ... more

T. Zhang; G.X. Zhang; K.P. Han; Y. Tang; J.Y. Wang; Q.C. Fan; X.S. Chen; Y. Wei; Y.J. Wang
CART gene; Goats; Microsatellites; Reproduction traits; SNPs

Polymorphisms of the CART gene were investigated by PCR-single-strand conformation polymorphism analysis in 540 samples from 10 goat breeds. Ten novel single-nucleotide polymorphisms as well as three microsatellites were detected; a mutation, 77T→C, led to an amino acid change (Leu→Ser). Associations between polymorphic loci and reproductive traits were analyzed in Chuandong White, ... more

P.Q. Wang; L.M. Deng; B.Y. Zhang; M.X. Chu; J.Z. Hou