Research Article

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12/21/2011
Alternative splicing; Dairy cattle; Lactoferrin gene; Mastitis

Bovine lactoferrin (bLF) is a member of the transferrin family; it plays an important role in the innate immune response. We identified novel splice variants of the bLF gene in mastitis-infected and healthy cows. Reverse transcription-polymerase chain reaction (RT-PCR) and clone sequencing analysis were used to screen the splice variants of the bLF gene in the mammary ... more

J.M. Huang; Z.Y. Wang; Z.H. Ju; C.F. Wang; Q.L. Li; T. Sun; Q.L. Hou; S.Q. Hang; M.H. Hou; J.F. Zhong
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Mastitis; PCR-PIRA; PCR-RFLP; Percentage of fat; PGLYRP-2 gene; Polymorphism

Peptidoglycan recognition protein 2 (PGLYRP-2), which belongs to the PGRP family, is the only member that has no direct bactericidal activity but has N-acetylmuramoyl-l-alanine amidase activity. This feature of PGLYRP-2 indicates that it may play an important role in eliminating the pathogen associated molecular pattern (PAMP), such as peptidoglycan (PGN), which can reduce ... more

H.L. Wang; Z.X. Li; L. Chen; J. Yang; L.J. Wang; H. He; F.B. Niu; Y. Liu; J.Z. Guo; X.L. Liu
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Dairy cattle; Lactoferrin; Mastitis; TLR4

Mastitis remains a major cattle disease with great global economic implications. Various approaches are currently employed in attempts to improve understanding of mastitis resistance and develop phenotypic markers for use in breeding programs (e.g., somatic cell score), including QTL discovery, wide-genome association studies, and identification of candidate genes related to immune ... more

A.M. Carvajal; P. Huircan; A. Lepori
08/29/2012
Alternative splicing; C4A gene; Dairy cattle; Mastitis; Transcription pattern

The complement system helps in the direct lysis of invading pathogens and modulates phagocytic, humoral and cellular immune responses. Complement 4 is a critical component in complement activity and protection against many bacterial pathogens because it is essential to classical and lectin activation pathways. We used reverse transcription and PCR to investigate alternative splicing and ... more

Y. Yang; J.M. Huang; Z.H. Ju; Q.L. Li; L. Zhou; R.L. Li; J.B. Li; F.X. Shi; J.F. Zhong; C.F. Wang
03/16/2012
ATP1A1; Dairy cow; K+-ATPase; Mastitis; Na+; SNP; Somatic cell score

Mastitis affects the concentrations of potassium and sodium in milk. Since sodium-potassium adenosine triphosphatase (Na+, K+-ATPase) is critical for maintaining the homeostasis of these two ions, and is involved in cell apoptosis and pathogenesis, we presumed that polymorphism of the ATP1A1 gene, which encodes the bovine Na+, K+-ATPase ... more

Y.X. Liu; C.H. Xu; T.Y. Gao; Y. Sun
01/16/2015
DNA; Extraction; Mastitis; Real-time polymerase chain reaction; Staphylococcus aureus

The aim of this study was to evaluate the repeatability and performance of 4 methods of extracting DNA from Staphylococcus aureus (SAU) and the gene encoding bovine mitochondrial cytochrome B (BMCB) in milk samples from cows with subclinical mastitis for use in amplification by real-time polymerase chain reaction. Two milk samples were obtained from cows naturally infected with ... more

A.G. Dibbern; B.G. Botaro; M.P. Viziack; L.F.P. Silva; M.V. Santos
2017 Mar 22
Actins; Animal Feed; Animals; Cattle; Chemokine CCL5; Diet; Female; Interleukin-8; Lactalbumin; Lactation; Mammary Glands, Animal; Milk; Milk proteins; Random Allocation; Rumen

In order to evaluate the milk yield, milk quality, and health of dairy cows fed a high-concentrate (HC) diet, eight lactating Holstein dairy cattle were randomly assigned to HC or low-concentrate (LC) diet groups and fed for 50 days, and the auto-control studying before and after treatment with the two diets was used. During the experiment, plasma and milk samples were collected and measured. ... more

Z.L. Xie; J. Zhang; D.M. Zhang; J.F. Li; Y.H. Lin
01/28/2018
Bulgarian Rhodopean cattle; Genetic polymorphism; Milk proteins; αS1 - casein

Milk protein genetic polymorphisms of the genus Bos provoke a significant scientific interest, mainly associated with their evolution, population structure, breeding and hybridization. The aim of present study is to investigate the influence of the genetic variants of αS1- casein gene with respect to milk production and quality of native for Bulgaria cattle breed ... more

P. Hristov; D. Teofanova; A. Georgieva; G. Radoslavov
12/19/2013
MALDI-TOF MS; Musa acuminate Colla; Proteomics; Two-dimensional gel electrophoresis

To establish a proteomic reference map of Musa acuminate Colla (banana) leaf, we separated and identified leaf proteins using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry (MS). Tryptic digests of 44 spots were subjected to peptide mass fingerprinting (PMF) by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS. Three ... more

Y. Lu; Y.X. Qi; H. Zhang; H.Q. Zhang; J.J. Pu; Y.X. Xie
07/04/2014
Cocoa; Plant protein extraction; Root; Two-dimensional gel electrophoresis

Theobroma cacao is a woody and recalcitrant plant with a very high level of interfering compounds. Standard protocols for protein extraction were proposed for various types of samples, but the presence of interfering compounds in many samples prevented the isolation of proteins suitable for two-dimensional gel electrophoresis (2-DE). An efficient method to extract root proteins ... more

F.Z. Bertolde; A.A.F. Almeida; F.A.C. Silva; T.M. Oliveira; C.P. Pirovani

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