Research Article

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Breeding; Core subset; Genetic diversity; Germplasm; Manihot esculenta Crantz; Molecular markers

Single nucleotide polymorphism (SNP) markers were used in the largest cassava (Manihot esculenta Crantz) germplasm collection from Brazil to develop core collections based on the maximization strategy. Subsets with 61, 64, 84, 128, 256, and 384 cassava accessions were selected and named PoHEU, MST64, PoRAN, MST128, MST256, and MST384, respectively. All the 798 alleles identified ... more

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Breeding; Genetic diversity; Genetic resources; Manihot esculenta Crantz; Ward-MLM

We evaluated the genetic variation of cassava accessions based on qualitative (binomial and multicategorical) and quantitative traits (continuous). We characterized 95 accessions obtained from the Cassava Germplasm Bank of Embrapa Mandioca e Fruticultura; we evaluated these accessions for 13 continuous, 10 binary, and 25 multicategorical traits. First, we analyzed the accessions based ... more

E.J. Oliveira; O.S.Oliveira Filho; V.S. Santos
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Araceae; Breeding; DNA markers; Genetic diversity; Germplasm; Ornamentals

We characterized single primer amplification reaction (SPAR) molecular markers from 20 genotypes of Anthurium andraeanum Lind., including 3 from commercial varieties and 17 from 2 communities in the State of Espírito Santo, Brazil. Twenty-four SPAR, consisting of 7 random amplified polymorphic DNA and 17 inter-simple sequence repeat markers were used to estimate the genetic ... more

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08/08/2011
Germplasm; RAPD analysis; ULCV; Urdbean

Urdbean (Vigna mungo) is an important pulse crop grown worldwide. Urdbean leaf crinkle virus (ULCV) is a pathogen of urdbean found in Pakistan that causes huge losses in yield. Forty urdbean varieties/lines were screened against the virus under field conditions during spring season 2009. None of the lines appeared to be highly resistant or resistant. On the basis of a 0-5 disease ... more

R. Binyamin; A. Khan; A.I. Khan; A. Khan; F.S. Awan; N.A. Khan
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Genotyping; Germplasm; Molecular markers; Simple sequence repeats; Tvv1; Vitis spp

Identification and knowledge concerning genetic diversity are fundamental for efficient management and use of grapevine germplasm. Recently, new types of molecular markers have been developed, such as retrotransposon-based markers. Because of their multilocus pattern, retrotransposon-based markers might be able to differentiate grapevine accessions with just one pair of primers. In order ... more

G.C. Sant’Ana; J.L. Ferreira; H.S. Rocha; A. Borém; M. Pasqual; G.M.A. Cançado
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Breeding; Corn silage; Male sterility; Meiotic mutation; ms17

Cytological analysis under light microscopy of the single hybrid P30R50 of silage corn revealed an abnormal pattern of microsporogenesis that affected the meiotic products. Meiosis progressed normally until diakinesis, but before migration to the metaphase plate, bivalents underwent total desynapsis and 20 univalent chromosomes were scattered in the cytoplasm. At this stage, meiocytes ... more

M.S. Pagliarini; V.F. Souza; N. Silva; C.A. Scapim; M. Rodovalho; M.V. Faria
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Brachiaria; Breeding; Forage grass; Hybridization; Meiosis

Brachiaria humidicola is a grass adapted to seasonally swampy grasslands in Africa; two cultivars, ‘common’ and Llanero, are widely used in Brazilian pastures. New cultivars are in great demand in order to diversify current production systems to achieve improved quality and yield. Cytological analyses of 55 accessions of this species available from the Embrapa Beef Cattle ... more

K.R. Boldrini; E.V. Adamowski; N. Silva; M.S. Pagliarini; C.B. Valle
10/19/2010
Breeding; Hybrids; Meiosis; Silage corn

In breeding programs, commercial hybrids are frequently used as a source of inbred lines to obtain new hybrids. Considering that maize production is dependent on viable gametes, the selection of populations to obtain inbred lines with high meiotic stability could contribute to the formation of new silage corn hybrids adapted to specific region. We evaluated the meiotic stability of five ... more

V.F. Souza; M.S. Pagliarini; C.A. Scapim; M. Rodovalho; M.V. Faria
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Breeding; Carica papaya L.; Genetic resources; Genetic variability; Gower algorithm

We examined the genetic diversity of papaya (Carica papaya) based on morpho-agronomic and molecular data. Twenty-seven genotypes grown in Brazil were analyzed with 11 AFLP primer combinations, 23 ISSR markers, 22 qualitative, and 30 quantitative descriptors. For the joint analyses, we used the Gower algorithm (Joint Gower) and the average value of the individual dissimilarity ... more

O.N. de Jesus; J.P.X. de Freitas; J.L.L. Dantas; E.J. de Oliveira
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Breeding; Genetic diversity; Phaseolus vulgaris; SSR markers

The identification of germplasm genetic variability in breeding programs of the common bean (Phaseolus vulgaris) is essential for determining the potential of each combination of parent plants to obtain superior genotypes. The present study aimed to estimate the extent of genetic diversity in 172 lines and cultivars of the common bean by integrating five tests of value for ... more

P.C.B. Cardoso; M.M. Veiga; I.P.P. de Menezes; P.A.M.R. Valdisser; T.C.O. Borba; L.C. Melo; M.J. Del Peloso; C. Brondani; R.P. Vianello

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