Research Article

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12/10/2013
Microsatellite; Oplegnathus fasciatus; Polymorphism

The rock bream fish Oplegnathus fasciatus is one of the most popular aquaculture species in China. In the present study, 15 novel polymorphic microsatellite loci were isolated and characterized from a wild population of O. fasciatus from the Zhoushan coast of China. The number of alleles per polymorphic locus ranged from 4 to 9 in a sample of 30 individuals. Observed and ... more

X.X. Sun; D.D. Xu; B. Lou; S.L. Li; W. Zhan; Y.R. Zhang; J. Xin
12/10/2013
COMT; Endometrial cancer; Meta-analysis; Polymorphism

Catechol-O-methyltransferase (COMT) is a critical enzyme to detoxify the carcinogenic catechol estrogen and the Val158Met polymorphism of COMT could influence its enzymatic activity. Recent epidemiological studies have investigated the correlation of COMT Val158Met polymorphism with endometrial cancer risk; however, the results are inconsistent. To better evaluate the role of ... more

G. Lin; J. Zhao; J. Wu; A. O; W.H. Zhang; Y. Zhang; L. Yu
12/04/2013
Microsatellite; Nibea albiflora; Polymorphism

Eleven novel microsatellite loci were isolated from a (CA)10-enriched genomic DNA library of Nibea albiflora. The characteristics of these microsatellites were determined in a sample of 48 N. albiflora individuals. The number of alleles at the 11 microsatellite loci ranged from 5 to 25, with an average of 13.5 per locus. The observed and expected ... more

D.D. Xu; B. Lou; S.L. Li; Y.R. Zhang; J. Xin; W. Zhan
12/04/2013
Cluster; Fenugreek; Genetic diversity; Polymorphism; RFLP; SDS-PAGE

Protein and DNA polymorphismswere surveyed among seven accessions of wild fenugreek (Trigonellafoenum-graecum L.) to estimate their genetic diversity and relationships. Samples were obtained from diverse ecogeographical areas in Saudi Arabia and Yemen. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of seed storage protein showed genetic variations ... more

E.A. Haliem; A.A. Al-Huqail
11/22/2013
Microsatellite; Molecular markers; Polymorphism; Portunus trituberculatus

The Swimming crab Portunus trituberculatus (Portunidae) is an important economically food species. To provide molecular markers for P. trituberculatus, we isolated and characterized polymorphic microsatellite markers. We developed a 5'-anchored genomic library of P. trituberculatus DNA, and derived 45 positive clones. We designed 30 pairs of primers from the ... more

L.P. Ren; Y. Qin; X.C. Li; Y.N. Sun; R.X. Wang
11/22/2013
Fatty acid composition; Insertion/deletion mutation; Polymorphism; Simmental bulls; Snow Dragon black cattle; SREBP1 gene

The sterol regulatory element binding factor 1 gene (SREBP1) plays an important role in the biosynthesis of fatty acids and cholesterol, and in lipid metabolism. The objective of this study was to investigate the effect of genetic polymorphisms of SREBP1 on the fatty acid composition of muscle and carcass traits in Simmental bulls and Snow Dragon black cattle. The 84-bp ... more

L. Xu; L.P. Zhang; Z.R. Yuan; L.P. Guo; M. Zhu; X. Gao; H.J. Gao; J.Y. Li; S.Z. Xu
11/18/2013
Chemokine; Gene; Inflammation; OSCC; Polymorphism

We aimed to evaluate the effect of genetic variants of the chemokine C-C motif receptor (CCR5) in the pathogenesis of oral squamous cell carcinoma (OSCC). A total of 127 patients diagnosed with OSCC and 104 healthy individuals were included in the study. The polymorphisms CCR5 59029 and CCR5-delta32 were assessed with the polymerase chain reaction-restricted fragment length polymorphism ( ... more

C.R. Tanyel; Z.B. Cincin; B. Gokcen-Rohlig; K. Bektas-Kayhan; M. Unur; B. Cakmakoglu
11/18/2013
High-altitude adaptation; Hypoxia adaptation; Polymorphism; Vascular endothelial growth factor A (VEGF-A); Yak

Vascular endothelial growth factor-A gene (VEGF-A) is a key regulator of angiogenesis and an endothelial cell mitogen that plays an important role in high-altitude adaptation. In this study, we detected 2 novel single-nucleotide polymorphisms (SNPs) of VEGF-A by screening for genetic variation in 700 individuals of 3 domestic Chinese yak breeds - namely Gannan (GN), ... more

X.Y. Wu; C.N. Liang; X.Z. Ding; X. Guo; P.J. Bao; M. Chu; W.B. Liu; P. Yan
10/30/2013
Dactylopiidae; Genetic markers; Opuntia ficus indica; Polymorphism

The carmine cochineal Dactylopius opuntiae is a key pest in productive fields of forage cactus in Pernambuco, Brazil. Species identification by means of molecular markers assists in understanding the genetic profile, underpins morphological characterization, and supports the monitoring of populations in integrated management programs designed to control this pest. We evaluated ... more

D.M.P. Silva; A.Cdo E.S. Mergulhão; L.V. de Medeiros; M.V.B. Figueiredo; H.A. Burity
10/24/2013
Chronic obstructive pulmonary disease; Meta-analysis; Polymorphism; Tumor necrosis factor-alpha

Chronic obstructive pulmonary disease (COPD) is a chronic systemic inflammatory disease; increasing evidence indicates that the TNF-α polymorphism is associated with progression of this disease. Few studies have focused upon association between TNF-α -238G/A or -863C/A polymorphism and COPD risk. Reported associations have been controversial because of small sample size and varied study ... more

K. Cui; X.Y. Ge; H.L. Ma

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