Research Article

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To understand the mechanism underlying the sudden animal death caused by acute heart failure during heat stress, the relationships among the heat-induced pathological changes and apoptosis and the variations in the levels of protective Hsp90α and its mRNA in the heat-stressed primary myocardial cells of neonatal rats in vitro were studied by cytopathological observation, ... more

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Heat shock factor-1; Heat shock protein 60; Heat shock protein messenger RNA; Heat stress; Myocardial cells

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Heat stress; Hsp60; In vivo and in vitro cell culture; Myocardial cell; Rat

To investigate the protective role of Hsp60 against stress damage and its role in the sudden death of stressed animals, changes in the levels of Hsp60 protein and hsp60 mRNA of myocardial cells in vivo and in vitro were studied. In addition, the relationship between Hsp60 expression and heat-induced damage was also studied. Rats were exposed to a temperature of ... more

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A.P. Del Vesco; E. Gasparino; V. Zancanela; D.O. Grieser; S.E.F. Guimarães; C.S. Nascimento; D.M. Voltolini; J. Constantin; F.S. Gasparin
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We evaluated the adenine nucleotide translocator (ANT) and cytochrome oxidase subunit III (COX III) mRNA expressions in the muscle and liver of Japanese quails presenting high and low feed efficiency (FE), and subjected them to three different environmental temperatures: comfort, heat stress (38°C), and cold stress (10°C). ANT mRNA expression was lower in the liver of heat-stressed ... more

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Creatine kinase; Heat shock protein 90 alpha (Hsp90α); Heat stress; Primary myocardial cells

The objective of this study was to investigate the mechanism of heat shock protein 90 alpha (Hsp90α) protection against heart damage resulting from heat stress by detecting Hsp90α mRNA, Hsp90α protein, protein localization, and cell damage in primary myocardial cells of neonatal rats in response to heat stress in vitro. The cells were heat-stressed at 42°C in an incubator with ... more

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