Research Article

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12/21/2010
Biodiversity conservation; Coefficient of similarity; genetic breeding; Genetic variability; Grouping analyses; Molecular marker

The genetic diversity and characteristics of commercial interest of Passiflora species make it useful to characterize wild germplasm, because of their potential use for fruit, ornamental and medicinal purposes. We evaluated genetic diversity, using RAPD markers, of 32 genotypes of Passiflora cincinnata collected from the wild in the region of Vitória da Conquista, Bahia ... more

C.B.M. Cerqueira-Silva; L.D.H.C.S. Conceição; E.S.L. Santos; C.B. Cardoso-Silva; A.S. Pereira; A.C. Oliveira; R.X. Corrêa
10/26/2010
Coefficient of similarity; Conservation; genetic breeding; Genetic variability; Grouping analyses; Molecular marker

In spite of the importance of and the considerable variability observed in Passiflora (Passifloraceae), little is known about the genetic diversity of most of the species of this genus. We evaluated the genetic diversity by RAPD markers in 18 genotypes of Passiflora trintae. The 15 primers generated 112 markers, 84% of which were polymorphic. The genetic distance ... more

C.B.M. Cerqueira-Silva; C.B. Cardoso-Silva; E.S.L. Santos; L.D.H.C.S. Conceição; A.S. Pereira; A.C. Oliveira; R.X. Corrêa
03/22/2012
Conservation; genetic breeding; Genetic variability; Grouping analyses; Molecular marker; Similarity coefficient

Little is known about the molecular genetic diversity of most Passiflora species. We used RAPD markers to evaluate the genetic diversity of 24 genotypes of the ‘sleep’ passion fruit (Passiflora setacea). Twelve primers generated 95 markers, 88% of which were polymorphic. The genetic distance estimated by the complement of the Dice index ranged from 0.29 (among accessions ... more

C.B.M. Cerqueira-Silva; E.S.L. Santos; L.D.H.C.S. Conceição; C.B. Cardoso-Silva; A.S. Pereira; A.C. Oliveira; R.X. Corrêa
12/08/2011
DNA sequence; Gene-rich region; Molecular marker; Resistance; Yellow rust

Analysis of DNA sequence variation among genotypes is useful for differentiation of wheat accessions, selection strategies and genetic development of crop plants. We screened molecular markers for yellow rust resistance genes (Yr7, Yr9, Yr15, Yr18, Yr26, and YrH52), which are in the gene-rich regions of wheat chromosomes 1B, 2B, and 7D ... more

E. Cabuk; Y. Aydin; A.A. Uncuoglu
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Genetic diversity; Molecular marker; Piper; RAPD; SRAP

Random amplified polymorphic DNA (RAPD) and sequence-related amplified polymorphism (SRAP) analysis were applied to 74 individual plants of Piper spp in Hainan Island. The results showed that the SRAP technique may be more informative and more efficient and effective for studying genetic diversity of Piper spp than the RAPD technique. The overall level of genetic ... more

Y. Jiang; J.P. Liu
10/06/2011
ISSR; Molecular marker; RAPD

Dalbergia oliveri is a leguminous tree of the Fabaceae family. This species is popular and valuable in Vietnam and is currently listed on the Vietnam Red List and on the IUCN Red List as endangered. Two PCR techniques using RAPD and inter-simple sequence repeat (ISSR) markers were used to make a comparative analysis of genetic diversity in this species. Fifty-six polymorphic primers (29 ... more

D.T. Phong; V.T.T. Hien; T.T.V. Thanh; D.V. Tang
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C. equisetifolia subsp incana; Casuarina equisetifolia subsp equisetifolia; Genetic diversity; Molecular marker; Northern coast of Senegal; RAPD

The genetic variation of 70 individual samples of Ca­suarina equisetifolia (L. Johnson) subsp equisetifolia and C. equiseti­folia subsp incana growing along the northern coast of Senegal was analyzed with RAPD markers. Of the 160 primers tested, five were chosen; they generated 1396 reproducible bands and 61 polymorphic bands that were scored. This ... more

A.L. Ndoye; O. Sadio; D. Diouf
07/13/2010
Genetic variability; Heliconia; Molecular marker; Phylogenetic relationships; RAPD; Zingiberales

The family Heliconiaceae contains a single genus, Heliconia, with approximately 180 species of Neotropical origin. This genus was formerly allocated to the family Musaceae, but today forms its own family, in the order Zingiberales. The combination of inverted flowers, a single staminode and drupe fruits is an exclusive characteristic of Heliconia. Heliconias are ... more

L.P. Marouelli; P.W. Inglis; M.A. Ferreira; G.S.C. Buso
05/04/2010
ISSR; Laboratory optimization; Method evaluation; Molecular marker; PCR

We evaluated the efficiency of the touchdown method to determine the ideal PCR conditions for distinct inter-simple sequence repeat primers for processing DNA from common corn, popcorn, sweet corn, and a Tripsacum-maize hybrid. Genomic DNA was extracted from eight accessions of corn: two of the dent type, one Tripsacum-maize hybrid, one sweet corn, one flint-type corn, ... more

E.C. Oliveira; A.T.Amaral Júnior; L.S.A. Gonçalves; G.F. Pena; S.P.Freitas Júnior; R.M. Ribeiro; M.G. Pereira
06/01/2010
Molecular marker; RAPD; Spittlebugs

Spittlebugs are the leading cause of damage to tall grasses. Annual losses are estimated to reach 2.1 billion dollars in sugarcane crops and grazing land throughout the world. Correct identification of these species is difficult due to similarities in color, body size and male genitalia. Molecular markers have been useful in the identification and assessment of genetic diversity of many ... more

A.M. Auad; R. Domingues; M.A. Machado; L.S. Souza; G.S. Carvalho; S.V. Paula-Moraes

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