Research Article

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Clone; Differential expression; Invariant chain; Muscovy duck; RACE; RT-PCR

The invariant chain (Ii) plays an important role as a chaperone for MHC II maturation and facilitates antigen presentation in vertebrates. We cloned, characterized and made a homology analysis of healthy adult muscovy duck Ii (MDIi), from a poultry farm in the suburban district of Hefei city in China, by rapid amplification of cDNA ends (RACE)-PCR and by measuring expression of the ... more

S.J. Liu; F.F. Chen; Y. Dai; C. Wu; Q.S. Ni; W.Y. Yu
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Clone; Duck; PCR-RFLP; PLIN gene; SNP

The perilipin (PLIN) gene is a candidate gene of carcass and fat traits in ducks. In order to study the molecular character of the PLIN gene and its function in different breeds of Chinese ducks, samples were obtained from the Chinese Academy of Agricultural Sciences Research Center for Birds, including 95 Peking ducks of the Z2 series, 91 Peking ducks of the Z4 series, ... more

H.L. Zhang; H.J. Fan; X.L. Liu; Y. Wu; S.S. Hou
10/10/2013
Differential expression; Duchenne muscular dystrophy; Protein-protein interaction network

Duchenne muscular dystrophy (DMD), which is caused by mutations in the X-linked dystrophin gene, is a severe and progressive neuromuscular disease with no available cure. By integrating 2 microarray datasets from the Gene Expression Omnibus, we identified differentially expressed genes in 2 stages of DMD and systematically explored their potential disease-related mechanisms using a ... more

D. Wang; Z.J. Wang; X.X. Song; L.H. Pu; X. Li; Y. Wang
12/18/2014
Differential expression; KNOX transcription factor; Manihot esculenta; Storage root

In plants, homeodomain proteins play a critical role in regulating various aspects of plant growth and development. KNOX proteins are members of the homeodomain protein family. The KNOX transcription factors have been reported from Arabidopsis, rice, and other higher plants. The recent publication of the draft genome sequence of cassava (Manihot esculenta Krantz) has ... more

D. Guo; H.L. Li; X. Tang; S.Q. Peng
10/27/2014
Differential expression; Gastric cancer; MicroRNAs; Network analysis

To identify potential targets for the early treatment and prevention of gastric cancer, microRNA (miRNA) expression profiles of precancerous lesions of gastric cancer were investigated. The miRNA microarray dataset GSE24839 was downloaded from Gene Expression Omnibus (GEO) and included 10 Helicobacter pylori-related gastritis samples and 10 gastric intestinal metaplasia samples. ... more

X.W. Wang; Y. Wu; D. Wang; Z.F. Qin
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ANGPTL4; Association analysis; Differential expression; Intramuscular fat; Pigs

In a previous study, we investigated differences in gene expression in backfat between Meishan and Large White pigs and their F1 hybrids, Large White x Meishan, and Meishan x Large White pigs. One potential differentially expressed sequence tag from the mRNA differential display was a homolog of the human angiopoietin-like 4 (ANGPTL4) gene, which encodes a protein that ... more

Z.Q. Ren; W.J. Wu; W.H. Liu; R. Zheng; J.L. Li; B. Zuo; D.Q. Xu; F.E. Li; M.G. Lei; D.B. Ni; Y.Z. Xiong
10/09/2015
Adhesion factor gene expression; Lactic acid bacteria; Muscovy duck; Probiotic

The purpose of this study was to assess the suitability of lactic acid bacteria (LABs) isolated from Muscovy duck as a potential probiotic. Isolates were identified by targeted polymerase chain reaction and assessed in vitro for probiotic characteristics such as autoaggregation; surface-charge; hydrophobicity; tolerance to acidic pH, bile salts and protease; and expression of ... more

Z.L. Xie; D.P. Bai; L.N. Xie; W.N. Zhang; X.H. Huang; Y.F. Huang
06/13/2013
Goat; MicroRNA characteristics; Real-time quantitative PCR; Solexa deep-sequencing technology

MicroRNAs (miRNAs) are an important class of small noncoding RNAs that are highly conserved in plants and animals. Many miRNAs are known to mediate a myriad of cell processes, including proliferation and differentiation, via the regulation of some transcription and signaling factors, which are closely related to muscle development and disease. In this study, small RNA cDNA libraries of ... more

Y.H. Ling; J.P. Ding; X.D. Zhang; L.J. Wang; Y.H. Zhang; Y.S. Li; Z.J. Zhang; X.R. Zhang
03/27/2015
Oryza sativa L.; Real-time quantitative PCR; Salinity; Ubiquitin

To obtain accurate and reliable results for the expression of genes of interest using quantitative real-time polymerase chain reaction (RT-qPCR) techniques, it is necessary to normalize the data by comparing them to constitutive genes that exhibit uniform expression levels under experimental conditions. In this study, the stability of expression was evaluated for the following ten ... more

G.P. Moraes; L.C. Benitez; M.Ndo Amaral; I.L. Vighi; P.A. Auler; L.C. da Maia; V.J. Bianchi; E.J.B. Braga